Is immunohistochemistry a useful tool in the postmortem recognition of myocardial hypoxia in human tissue with no morphological evidence of necrosis?

Myocytes in the border zone of myocardial infarction are under severe hypoxia without characteristic morphology of necrosis, and show ultrastructural features similar to those seen within the first hours after coronary occlusion. This study was carried out to evaluate the possibility that immunohistochemical methods could be used for the early diagnosis of myocardial infarction by detecting areas of hypoxia. Nineteen human sections of formalin-fixed paraffin-embedded myocardial samples showing a necrotic area and its border were submitted to immunohistochemical staining with the markers antimuscle actin, antimyoglobin, antitroponin T, antifibronectin, and anticomplement component C9. Sections were also subjected to azan trichrome and hematoxylin-basic fuchsin-picric (HBFP) staining techniques. Immunohistochemistry and azan trichrome showed that in the border zone there was a pattern of reaction intermediate between the infarcted area and the normal myocardium. The HBFP failed to distinguish these two areas. In conclusion, immunohistochemistry and azan trichrome can recognize myocardial hypoxia. Because hypoxia is an invariable condition in infarction, these techniques can be used to confirm suspected cases of myocardial infarction in which necrosis is not yet evident. However, considering that agonal states may be associated with generalized hypoxia, further studies are needed to confirm the reliability of this procedure in the earlier phases of myocardial infarction.     

The ASEAN free trade area and the construction of a Southeast Asian economic community in East Asia

In October 2003, ASEAN leaders decided to establish an ASEAN Economic Community (AEC) by 2020. An AEC is presented by advocates as a logical step following the completion of the ASEAN Free Trade Area (AFTA) by ASEAN6 in 2003. Adopting a critical political economy approach inspired by the work of Mitchell Bernard and Robert W. Cox, this article argues that the decision to launch AFTA and an AEC are motivated primarily by the desire to transform Southeast Asia into an investment site and a production base for the world market within East Asia, in competition with China. AFTA and a future AEC are decisions taken within the structural context of an East Asian region characterised, among others, by the organisation of Japanese production and the developmental state.     

Y-chromosome STRs in an Antioquian (Colombia) population sample

Haplotype data were obtained from a sample of 777 unrelated male individuals from Antioquia Department (Colombia), for eight Y-chromosome STRs (DYS19, DYS385, DYS389 I, DYS389 II, DYS390, DYS391, DYS392 and DYS393). A total of 442 different haplotypes were identified of which 334 were represented only once in the database and the most frequent haplotype was found in 32 individuals. A high haplotype diversity was found (99.45%). Genetic distances were calculated using previously published haplotype data and the lowest values were found for the comparisons with samples of lberian origin.     

Corruption and local politics in Chile

This paper argues that the greater openness of Chilean politics to public scrutiny and the penetration of the locality by the market have made corruption a more visible phenomenon. Presenting empirical evidence collected for nearly fifty cases of allegations of municipal corruption, the paper explores the content, actors, directionality, levels of corruption and the links existing with the local political environment: partisan composition of councils, political affiliation of mayors and sources denouncing corruption. The conclusions suggest that although many accusations of corruption may correspond to particularistic claims comprehended in Chile's traditional system of political clientelism, a significant difference with the past is the inclusion of private business as a new component of an old network of political clientelism.This is a revised version of an exploratory paper delivered at the International Seminar El Estado y la prevención de la corrupción organized by the Institute of Political Science. The Catholic University of Chile with the support of the Presidential Commission on Public Ethics, held in Santiago de Chile, July 4–5, 1994. The opinions, findings and conclusions herewith included are the author's own responsibility and do not represent the official standing of any of the above mentioned institutions.     

EU fta Negotiations with sadc and Mercosur: integration into the world economy or market access for EU firms?

The EU claims that its free trade agreements with regional organisations of developing countries can promote the respective regions' integration into the world economy. Taking as case studies EU negotiations with the Southern African Development Community and Mercosur, the paper argues that the EU and its partners have different conceptions of integration into the world economy. For the EU the latter simply means multilateral trade liberalisation under the wto, while, for its partners, it involves increasing industrial production and exports of manufactured products. If the latter notion is accepted, an fta with the EU should increase European foreign direct investment into the region or at least increase their trade surpluses, thus increasing the resources available for support of local firms. The paper argues that an fta with the EU will not be likely to produce these results; thus the fta will simply be an instrument to promote market access for EU firms.     

Administrative Innovation and Culture： Pillars of the Competitive Strategies in Horticultural Companies of Sinaloa

In contemporary times, a fundamental aspect for organization has been to achieve innovation through culture and approach to encourage the search and development of new ideas, new products, and new services; this is a key point to fulfill the competitive advantages which a company needs to be successful in the global market. The Sinaloa horticultural company has more than 100 years＇ history in the United States export market, and is presenting new challenges and demands from the market in order for it to achieve its permanence. The objective of this research is to identify and propose different strategies to implement management innovation, which in its essence is the one that coordinates, directs, and controls the human resource considering culture as an integral part, with a capacity to generate the spark and function as a motivating source which drives companies to consolidate and integrate supply chains.     

Discriminant function analysis for sex assessment in pelvic girdle bones: sample from the contemporary Mexican population

Sex assessment of skeletal remains plays an important role in forensic anthropology. The pelvic bones are the most studied part of the postcranial skeleton for the assessment of sex. It is evident that a population-specific approach improves rates of accuracy within the group. The present study proposes a discriminant function method for the sex assessment of skeletal remains from a contemporary Mexican population. A total of 146 adult human pelvic bones (61 females and 85 males) from the skeletal series pertaining to the National Autonomous University of Mexico were evaluated. Twenty-four direct metrical parameters of coxal and sacral bones were measured and subsequently, sides and sex differences were evaluated, applying a stepwise discriminant function analysis. Coxal and sacra functions achieved accuracies of 99% and 87%, respectively. These analyses follow a population-specific approach; nevertheless, we consider that our results are applicable to any other Hispanic samples for purposes of forensic human identification.     

The Need for an Interdisciplinary Approach in Forensic Sciences: Perspectives from a Peculiar Case of Mummification

The finding of a mummified body raises many problems, also because of the limits of the medico‐legal investigations in case of mummification. Psychological autopsy and behavioral analysis have demonstrated a significant impact in case of equivocal death. The mummified corpse of a woman was found sealed in a wardrobe during the death investigation of a 36‐year‐old man, later discovered to be the woman's son. The woman's corpse was well preserved and no external injuries were found. Autopsy could not ascertain the cause of death. The state of the premises and the writings on the walls offered an opportunity to investigate the man's psychological profile and to better understand how the events might have taken place. The role of an accurate investigative analysis of the crime scene is a cornerstone of forensic pathology and the case presented underlies the importance of an interdisciplinary approach in forensic sciences.