Extraction and analysis of human nuclear and mitochondrial DNA from electron beam irradiated envelopes

The United States Postal Service is considering methods such as electron beam irradiation to neutralize biological agents sent through the mail. While this is proven to reduce/eliminate pathogenic organisms, it may also degrade human genomic DNA and therefore hinder the ability to garner forensically informative genetic profiles. To determine the effects of electron beam irradiation on DNA typing, 16 white, standard letter-sized envelopes were licked. Half of the envelopes served as nonirradiated controls while the other half underwent irradiation at dosages sufficient to kill anthrax spores (29.3 and 51.6 kGy). Total cellular DNA was extracted from all envelopes; nuclear short tandem repeat loci, as well as the hypervariable region I from mitochondrial DNA, were amplified by means of the polymerase chain reaction. Short tandem repeat profiles and mitochondrial DNA sequence haplotypes were acquired on an ABI Prism 310 Genetic Analyzer platform. Analysis of data from irradiated samples revealed evidence of DNA degradation; however, the ability to construct full genetic profiles from both nuclear and mitochondrial DNA remained largely unaffected. The use of the polymerase chain reaction, coupled with florescent fragment analysis and mitochondrial DNA sequencing, should be considered to profile biological material from evidence enduring irradiation to inactivate infectious agents.     

Genetic profile of the Ecuadorian Mestizo population (Ecuador-South America) by using the Power Plex 16 System Kit

Allele frequency data for the 15 STR systems and Amelogenine were determined in a population sample of healthy Mestizo unrelated individuals. All loci met Hardy-Weinberg expectations and the high discrimination power of combined system showed the forensic efficiency of these genetic markers. There is a lack of information on Ecuadorian population from a genetic point of view and therefore no previous publications on the distribution of STRs on Mestizos is available. Mestizos are descendents of Spanish and Amerindian people, however, significant differences were found between the former and the populations from Spanish peninsula, that have been analyzed for these genetic markers.     

An unusual case of railway suicide

A rare case of suicide in which the victim had been lying along the railway track in a supine position and with extended extremities is described. The wheels of a train caused longitudinal hemisection with complete evisceration. Epidemiological data on train suicides are given and the relationship between this method of suicide and mental illnesses and consumption of alcohol are discussed.     

Layers of decision: Linking social definitions of corruption and willingness to take action

The literature available about social definitions of corruption is surprisingly discrete from that which addresses the issue of whistleblowing or the reporting of crime and corruption. The current study, however, empirically links the two. A survey of more than 1300 public sector employees was undertaken to explore: i) how and why public sector employees define some behaviours as corrupt; ii) factors which may hinder public sector employees taking action about corruption which they may observe at work; and iii) the link between i) and ii). The study found that within the New South Wales (NSW) public sector, views about what constitutes corruption are diverse and that this has a significant, though not exclusive, impact upon the action respondents said they would take in response to workplace corruption. The study discusses other factors which also impact upon the stated decision not to take action about corruption and suggests some possible courses of action for addressing these factors.The research upon which this paper is based formed part of the research program of the Independent Commission Against Corruption.     

Migration policies and population distribution in Mexico: regional implementation and impact

The author begins by analyzing the legal framework of migration and population redistribution policies in Mexico since 1973, when the General Law on Population was passed. In that framework, she points out several institutional deficiencies and contradictions. In the second section, she discusses demographic planning in this field, and conducts a follow-up on implementation based on reports from the institutions involved and official documents. To conclude, she examines practical applications of policy instruments, comparing the behavior of governmental variables that had a bearing on the phenomenon before and after the implementation of said policy (1970 to 1990). One of her findings is that the basic elements making population policies on migration operational did not move in the desired direction, i.e., to modify migratory and population distribution so as to achieve expected goals. Despite the continuity of population policies on migration, the implementation of such policies still poses diverse obstacles.     

Vertical Competition in Unitary States: The Case of Italy

In unitary states – states in whichconstitutional powers are owned by thecentral government – vertical competitioncan generate stable outcomes, that isoutcomes that do not unravel througharbitrary repossessions by the center.Stability is a product of institutionalcommitment devices. Through these ademocratic government, though it cannotbind successor governments, can effectivelyoblige them to respect some of thedecisions it has made. A number of suchcommitment devices exist in Italy; theymake possible stable vertical competitionbetween central and regional governments.