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Schneider PM Bender K Mayr WR Parson W Hoste B Decorte R Cordonnier J Vanek D Morling N Karjalainen M Marie-Paule Carlotti C Sabatier M Hohoff C Schmitter H Pflug W Wenzel R Patzelt D Lessig R Dobrowolski P O'Donnell G Garafano L Dobosz M De Knijff P Mevag B Pawlowski R Gusmão L Conceicao Vide M Alonso Alonso A García Fernández O Sanz Nicolás P Kihlgreen A Bär W Meier V Teyssier A Coquoz R Brandt C Germann U Gill P Hallett J Greenhalgh M 《Forensic science international》2004,139(2-3):123-134
Degradation of human DNA extracted from forensic stains is, in most cases, the result of a natural process due to the exposure of the stain samples to the environment. Experiences with degraded DNA from casework samples show that every sample may exhibit different properties in this respect, and that it is difficult to systematically assess the performance of routinely used typing systems for the analysis of degraded DNA samples. Using a batch of artificially degraded DNA with an average fragment size of approx. 200 bp a collaborative exercise was carried out among 38 forensic laboratories from 17 European countries. The results were assessed according to correct allele detection, peak height and balance as well as the occurrence of artefacts. A number of common problems were identified based on these results such as strong peak imbalance in heterozygous genotypes for the larger short tandem repeat (STR) fragments after increased PCR cycle numbers, artefact signals and allelic drop-out. Based on the observations, strategies are discussed to overcome these problems. The strategies include careful balancing of the amount of template DNA and the PCR cycle numbers, the reaction volume and the amount of Taq polymerase. Furthermore, a careful evaluation of the results of the fragment analysis and of automated allele calling is necessary to identify the correct alleles and avoid artefacts. 相似文献
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Nicholas Faulkner Kim Borg Peter Bragge Jim Curtis Eraj Ghafoori Denise Goodwin Bradley S. Jorgensen Lena Jungbluth Sarah Kneebone Liam Smith Breanna Wright Paula Wright 《Public administration review》2019,79(1):125-135
Public administrators rely on written communications to send information to citizens and stakeholders, and they are among the heaviest users of the postal service. Behavioral science research has identified several techniques that public administrators can use to increase compliance with written requests and, in turn, increase effectiveness. Currently, however, many written communications from government bodies are not written in a manner that utilizes these techniques. It remains an ongoing challenge for public administrators to identify, understand, and use these techniques in the written communications sent by their organizations. This article presents a framework capturing seven prominent techniques in a simple mnemonic—INSPIRE—that is already being used by several government bodies in Australia. It also provides practical examples of how to use each technique and demonstrates that using these techniques could result in large aggregate improvements in effectiveness and socially desirable outcomes of public administrators' written communications. 相似文献
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This article outlines an innovative project to encourage knowledge sharing and engagement between academics and a policy team within the Department of the Prime Minister and Cabinet (PM&C). The project was designed to enhance policy advice by drawing on a group of academics outside the policy ranks to act as a sounding board, to question, and provide differing perspectives within the policy process of crafting advice to government. External expertise and particularly academic research has the potential to improve evidence‐based policy but often fails to be specific or timely for those at the “pointy end” of policy decision making. An informal knowledge sharing framework has the potential to improve the exchange of information through confidential and targeted conversations between researchers and public servants as one way of overcoming these barriers. 相似文献
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Only the best manufacturers will remain competitive in today's challenging environment. Any manager involved with developing new products knows the excitement, complexity, and risk of this unpredictable undertaking. Based upon findings from four world class manufacturers of medical imaging technology in Japan and the United States, this article presents a new organizing framework for integrating external information into the NPD process. The findings are particularly timely in an era when manufacturers are vulnerable to intense global competition. The article concludes with several implications for managers involved in the new product development process. 相似文献
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