Typing the 1.1 kb control region of human mitochondrial DNA in Japanese individuals

This study presents a reliable method that uses high-fidelity long-range PCR and optimized primers to assess polymorphism and to genotype human mitochondrial DNA (mtDNA). This method was used to analyze polymorphic sites in the human mtDNA control region, including hypervariable regions I, II, and III (HVI, HVII, and HVIII), from 124 unrelated Japanese individuals. In HVI, HVII, and HVIII, 80, 37, and 14 polymorphic sites were identified, respectively, excluding those in the homopolymeric cytosine stretch (C-stretch) regions. The region between HVI and HVII also contained 15 polymorphic sites. On the other hand, C-stretch length heteroplasmy in HVI or HVII was observed in 66 of 124 Japanese individuals (53%), which is much higher than in Caucasian populations. The variants in the C-stretch regions were characterized by counting the number of heteroplasmic peaks split from the single peak in homoplasmic sequences (i.e., 16244G and 16255G in HVI and 285G in HVII). Including the C-stretch length heteroplasmy, the 124 Japanese mtDNA samples were classified into 116 distinct haplotypes. The random match probability and the genetic diversity were estimated to be 0.95% and 0.998581, respectively, indicating that the method presented here has higher discrimination than the conventional method for mtDNA typing using HVI and HVII. [Correction added after publication 30 January 2007: in the preceding sentence random match probability and genetic diversity estimates were corrected from 0.95 and 0.998581%, respectively, to 0.95% and 0.998581, respectively.] The haplogroups and their frequencies observed in this study (i.e., D4; 13.7%, M7a1; 11.3%, D4a; 9.7% and M7b2; 8.9%) were similar to those observed in other studies of Japanese mtDNA polymorphism. The method described here is suitable for forensic applications, as shown by successful analysis of tissues from highly putrefied remains of an infant, which allowed maternal relationship to be determined via mtDNA haplotyping.     

Detecting Domestic Violence: Spanish External Validation of the Index of Spouse Abuse

The aims of the study were to assess the psychometric properties of the Spanish version of the Index of Spouse Abuse (ISA), and to validate it against external criteria of intimate partner violence. The Spanish version of the ISA was administered to 223 non-abused women and 182 victims of intimate partner violence. Internal consistency coefficients oscillated between 0.88 and 0.98. The Confirmatory Factor Analysis failed to replicate the original two-factor structure. Using Exploratory Factor Analysis, a two-factor solution was found: physical (ISA-P) and non-physical (ISA-NP), but the items included in each factor were slightly different from the original two subscales. Receiver operating characteristic curve analysis revealed an AUC value for the ISA global score of 0.99 (95% CI: 0.98–0.99), with the optimal cut-off of 12 for detecting intimate partner violence. The Spanish version of the ISA is a valid instrument for detecting intimate partner violence in a female population.     

The making of Narco bosses: hard drug dealing crimes among Mexican students

This study aimed at investigating the predictors of hard drug dealing crimes among Mexican students. Tests of difference and ordinal regression were employed to explore group differences and predictors of hard drug dealing crimes. The sample included 14,306 last-year high school students nationwide. Results show that 3.5% of the students surveyed admittedly reported to have been involved in a drug dealing crime of an illegal substance different from Marijuana. Several factors were found to decrease the odds of a student to get involved in a hard drug crime. Federal policy implications and solutions are provided.     

Contested Discourses on National Identity: Representing Nicaraguan Immigration to Costa Rica

This article explores the ways in which the immigration of Nicaraguans to Costa Rica is represented by Costa Rican institutions and individuals through public discourses and everyday life. Three discourses are considered. First, intellectual claims that immigration poses a threat to Costa Rican national identity, whereby intellectuals portray 'Costa Rica in crisis' due to immigration. Second, fictional works seek to represent issues regarding the Nicaraguan community in innovative ways, being open‐ended and including diverse voices. Third, stories written by Nicaraguan children are discussed in view of exploring how hostility towards immigration interpellates them, who face the challenge of negotiating their identities with their peers in everyday life. The article ends by asking for a politics able to defend public institutions and public investment, able to forge networks of solidarity with immigrant communities.     

Job Satisfaction among Primary School Personnel in Madagascar

This article analyses the job satisfaction of primary school teachers in Madagascar. Based on the estimation of multilevel models, low wages and problems getting paid, job insecurity, lack of in-service training, high pupil-teacher ratios, and lack of basic infrastructure and teaching materials are identified as the main reasons for dissatisfaction. Principals’ control of teachers’ activities also adversely affects satisfaction, suggesting that, in Malagasy schools, neither school directors nor teachers have succeeded in adopting organisational cultures based on cooperation among their members. These results are likely to stimulate debates on educational policy, both in Madagascar and in many other developing countries.     

The Impact of Juvenile Court Judge Ethical Orientation on Decision Making

This exploratory study examines the ethical ideologies of a sample of 76 juvenile court judges attending a conference using an Ethical Position Questionnaire. Potential effects of ethical orientation on judicial decision making are identified and discussed. The findings indicate a relatively even distribution of judges across ethical ideologies. The connection between ethical ideology and the prevailing juvenile justice philosophy across jurisdictions, and the importance and complexity of judicial education and training in juvenile justice, are discussed.