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141.
142.
Delamoye M Duverneuil C Riva K Leterreux M Taieb S De Mazancourt P 《Forensic science international》2004,143(1):47-52
Routine control of 2055 consecutive genotypes revealed discrepancies between the profiles established with the SGM plus and/or Profiler plus kits on one hand, and the profiles established with the Powerplex16 kit on the other hand. Furthermore, five discrepancies for vWA, three for D8S1179, two for FGA and three for D18S51 loci were found. In 10 cases (loci vWA, FGA, D18S51, D8S1179), the SGM plus and/or Profiler plus profiles showed homozygosity and the Powerplex16 genotype revealed heterozygosities which were confirmed to be true, both by typing with individual primer pairs and DNA sequencing. In four cases (two discrepancies at locus FGA, one at D18S51 and an abnormal paternity pattern for D5S818), the Powerplex16 kit showed apparent homozygosity and the SGM plus and/or Profiler plus kits showed heterozygosity. Mutation analysis could be performed for some of these individuals and evidenced variants, presumably leading to an annealing failure of one primer; the identified mutations are reported. It is suggested that databases should include information about the kits used to determine the profiles while ensuring that the primer sequences are made available. 相似文献
143.
Delamoye M Duverneuil C Paraire F de Mazancourt P Alvarez JC 《Forensic science international》2004,141(1):23-31
A new rapid and sensitive high-performance liquid chromatography (HPLC) method has been developed for the simultaneous identification and quantification in human plasma of the 13 most commonly prescribed beta-blockers and one active metabolite-atenolol, sotalol, diacetolol, carteolol, nadolol, pindolol, acebutolol, metoprolol, celiprolol, oxprenolol, labetalol, propranolol, tertatolol and betaxolol. It involves liquid-liquid extraction procedures followed by liquid chromatography coupled to photodiode-array UV detection with a fixed wavelength at 220 nm for quantification. Compounds were separated on a 5 microm Hypurity C(18) (ThermoHypersil) analytical column (250 mm x 4.6 mm, i.d.) using a gradient of acetonitrile-phosphate buffer pH 3.8 at a flow rate of 1.0 ml/min. The total analysis time was 26 min per sample. Extraction recoveries were between 74 and 113% for the polar compounds and between 20 and 56% for the most apolar compounds. Calibration lines were linear in the range from 25 to 1000 ng/ml for all compounds excepted carteolol and nadolol (50-1000 ng/ml), all of them with coefficients of determination (r2 values) >/=0.994. Limits of detection (LODs) ranged from 5 to 10 ng/ml. Intra-assay and inter-assay precision and accuracy were studied at two concentration levels (100 and 500 ng/ml). The intra-assay coefficients of variation (CVs) for all compounds were = 8.3% and all inter-assays CVs were below 12.6%. The intra-assay and inter-assay accuracies for all compounds were found to be within 91.4 and 105.6% at 100 ng/ml and within 94.1 and 107.4% at 500 ng/ml. Thus, the performance of the method described allows its use in toxicological screening and in quantification of the most prescribed beta-blockers drugs. 相似文献
144.
How does the expressed political ideology of voters influence their evaluation of presidential candidates? The classic answer to this question is provided by the spatial theory of electoral choice in which utility for a candidate is a function of the proximity between the voter and candidate positions on the liberal-conservative continuum. We have argued elsewhere that spatial theory, while intellectually appealing, is inadequate as an empirical model of mass behavior. We have developed a directional theory of issue voting that we believe provides a more realistic accounting of how specific policy issues influence utility for a candidate. Directional theory is based on the view that for most voters issues are understood as a dichotomous choice between two alternative positions. While ideology is widely understood as a continuum of positions, the directional model can be applied to the relationship between ideology and candidate evaluation. In this paper we compare the two theories using National Election Study data from 1972 to 1988. The results tend to favor the directional model over the traditional proximity model. We conclude by briefly tracing out the implications of this finding. 相似文献
145.
Charlotte Liddell 《Feminist Review(on-Line)》2005,79(1):69-82
This article looks at the way the Brazilian writer and educator Nísia Floresta addresses issues of race and class within her construction of nationhood. This is achieved through a consideration of the specific subject of maternal breast-feeding as discussed by Floresta in two texts, written in Brazil and France, respectively. A comparison of these works reveals a very different engagement with race and class factors in determining women's claim to citizenship. Floresta, in common with early 19th-century European feminism, believed this claim to lie within the domestic sphere, primarily in the role of mother and educator. The article considers how, as a vital aspect of that role, she portrays maternal breast-feeding as both natural and patriotic. It also highlights the extent to which Floresta was influenced by the prevailing concerns of her contemporaries regarding the risks of wet nursing and the threat posed by household slaves, before going on to consider how this discourse leads Floresta to exclude black slave women from her vision of patriotic motherhood, while acknowledging the maternity of women of all classes in a European context. Patriotic concerns, shaped by racial prejudices, overshadow Floresta's feminism, prohibiting the kind of unifying discourse of motherhood, which she claimed to profess. 相似文献
146.
Charlotte Dargie 《政治学》1998,18(1):65-71
Qualitative research in politics is predominantly interview-based. Observation is neglected as a research tool by political scientists. This article presents the case for using observation techniques in political research. Drawing from the authors experience of using structured observation to research Chief Executives, the article shows how observation can be included as a valuable part of a qualitative research strategy. It addresses the how, where, when and why of applying observation to political research. The crucial advantage of observation methods is identified as understanding the context and environment in which actions occur and decisions are taken. 相似文献
147.
This paper presents richer contemporaneous evidence of Congress's role in the passage of the Radio Act of 1927, the act which established the basic statutory framework that still governs federal regulation of broadcasting in the United States. Recent analysis finding the court's decision in Tribune Co. v. Oak Leaves Broadcasting Station to have been the cause of Congress's action on the radio bill is shown to rest on an inaccurate chronology of congressional decisionmaking. More closely examining the actions of legislators upon whose votes passage of the radio act depended, this paper contributes new evidence of strategic orchestration surrounding the perceived “chaos of the airwaves” that stimulated broadcasting regulation. Original congressional documents show that, in a political context characterized by costly information, intra-congressional manipulation of information costs was an important factor in the adoption of the Radio Act of 1927. Personal ties between executive branch officials are shown to have spawned a key legal opinion that prompted passage of the radio bill. 相似文献
148.
Ola Listhaug Stuart Elaine Macdonald George Rabinowitz 《Scandinavian political studies》1994,17(4):273-287
We examine how clearly citizens in Norway and the United States perceive the ideology and issue positions of parties and candidates. Following in the tradition of Campbell & Valen (1966) and Granberg & Holmberg (1988). we ask: Does the political system matter? We also consider the role of individual sophistication and whether sophistication has different consequences in the two cultural setting. Across a broad range of issues, we find that citizens in Norway have a sharper view of party positions than those in the United States. Sophistication influences the clarity of issue perception in both countries. However, the hypothesis that sophistication matters more in the US is not well supported. 相似文献
149.
Stuart Elaine Macdonald James W. Prothro George Rabinowitz Keith J. Brown 《Political Behavior》1988,10(2):117-135
Recent work in cognitive processing and individual decision making has emphasized that individuals vary in the criteria they bring to bear to evaluate political objects. In this paper we argue that individual differences are but one piece of the decision-making puzzle, and that environmental factors are also important. Of particular interest to us is the idea that different candidates evoke different evaluative criteria. Using data from a 1984 sample of undergraduates and the 1980 National Election Study, we find evidence that political evocation effects do exist. This result has implications for understanding both the decision calculus of individuals and the collective rationality of the electorate. 相似文献
150.
Ola Listhaug Stuart Elaine Macdonald George Rabinowitz 《Scandinavian political studies》1990,13(3):227-254
This article examines mass perceptions of political parties in Norway, Sweden, Denmark, West Germany, the Netherlands and France. For each country we construct a map in which the parties, voters, and demographic groups are located to provide a visual and spatial overview of the structure of electoral competition. Two dimensions are adequate for displaying the main ideological cleavages in each of the countries. In each case there is a strong left-right dimension combined with a more culturally defined and usually weaker second competitive dimension. In general, we find that no parties occupy the center areas of the space, which are usually dense with voters. This leads us to question the adequacy of the traditional spatial model of elections for describing competition in multiparty systems. 相似文献