Sample pre-treatment for CO-oximetric determination of carboxyhaemoglobin in putrefied blood and cavity fluid

Blood or blood-containing cavity fluid samples recovered from decomposed bodies in suspected carbon monoxide (CO) poisoning cases often have low total haemoglobin (tHb) levels or contain significant amounts of oily droplets, methaemoglobin (MetHb) and sulphaemoglobin (SHb), all conditions which render the validity of the measurements of carboxyhaemoglobin (HbCO) by commercially available CO-oximeters doubtful. Two sample pre-treatment methods, namely, centrifugal evaporation and addition of a commercially available standard haemoglobin solution containing a known level of HbCO, were developed to render such putrefied samples amenable to analysis by CO-oximeters. The methods developed were validated with samples of various degrees of putrefaction and various HbCO levels. The relative uncertainty of the determined HbCO levels as compared to their corresponding reference levels for the centrifugal evaporation method was approximately 5% and that for the standard addition method was <20%.     

Application of Volume‐of‐Fluid Method to Analyze the Viscosity Effect on the Spine Formation of Bloodstains

In bloodstain pattern analysis, the blood droplet volume and surface impact velocity play an important role, and many related experimental studies have been carried out. If an appropriate computational fluid dynamics (CFD) model that could solve bloodstain patterns, especially spine formation bloodstain patterns, can be obtained, the blood droplet volume and impact speeds at various crime scenes can be predicted more accurately. For this purpose, Flow‐3D software using the volume‐of‐fluid method was applied to analyze the behavior of human blood droplets during an impact event, especially focusing on the viscous effect on splashing, which forms the spine which can be used to predict the impact velocity. To obtain a non‐Newtonian viscosity model of blood for a computational fluid dynamic analysis, the venous blood samples of 163 people were tested using a hemorheology instrument. Among the venous blood samples of 163 people, 37 samples for which all blood test results were in a normal range were selected for the non‐Newtonian viscosity modeling. From the CFD analysis, it could be concluded that a non‐Newtonian viscosity model is more appropriate than a constant viscosity model for predicting splashing that forms the spine. The gradient of the non‐Newtonian model at a high shear rate has more of an effect on spine formation than that at a low shear rate. The lowest viscosity with a high velocity at the outer front of the radiating flow plays an important role in forming the splashing pattern.     

Introduction

The seemingly disparate aspects of long-term care (LTC) exist in a broader context of concern for the ability of disabled and chronically ill individuals of all ages to function in everyday life. A focus in LTC, therefore, is on the types of services and products needed for an individual to perform basic activities of daily living.

A primary emphasis in LTC is on the individual who is in need of assistance. Related questions include: What impact will the receipt of LTC have on the autonomy of the individual? How will the care, which may extend over a period of years or decades, be funded? How will the various payment mechanisms affect an individual's access to care? What are potential solutions to the problems encountered as the individual and his or her family deal with the fragmentation and other problems of the LTC system? Such questions are addressed in the articles in this symposium, “Issues in Long-Term Care.” This paper provides a context for the symposium.     

Between hearts and pockets: locating the outcomes of transnational homemaking practices among Mexican women in Canada's temporary migration programmes

Temporary migration programmes (TMPs) contain features such as reduced costs and the social legitimation of regularized entry that allow women, including the very poor, to access transnational livelihoods. For mothers, taking up opportunities for employment abroad inevitably involves ‘transnational homemaking’, the set practices involved in caring for family relationships and maintaining household economies across borders. In this article, we examine the transnational homemaking practices undertaken by rural Mexican migrant women employed in highly masculinized TMPs in Canada, tracing how they construct and maintain household economies across borders through a delicate (re)negotiation of reproductive roles and responsibilities with non-migrating kin in Mexico. We find that migration yields material and subjective benefits that enable the expansion of their citizenship across multiple dimensions ranging from the economic to the sexual. At the same time, as racialized, gendered, migrants from the global South, their labour and status in Canada are highly precarious. The advantages derived from transnational migration are thus tenuous, limited, and contradictory.     

The US–China Relationship and Asia-Pacific Security: Negotiating Change

Abstract

The crucial determinant of Asia-Pacific security is whether the U.S. and China can negotiate their relationship and their relative positions and roles in such a way as to produce sustainable regional stability. This paper examines three alternative scenarios of negotiating Sino-American coexistence: (I) The maintenance of the status quo of U.S. strategic dominance over the region, which China does not challenge; (II) negotiated change, by which the two powers coordinate to manage a more fundamental structural transformation; and (III) power transition, in which there is a significant structural shift in the regional system as a rising China challenges U.S. dominance, with a range of possible outcomes.     

Allegories of Venice: Singapore’s Vague Concept of a Global City

This paper argues that although the state elites of Singapore use “Venice” as an image to legitimate the People’s Action Party’s continuous rule and unpopular immigration policies, the image has both empowered and constrained the state. To the state, Venice serves as a keyword that conjures up dynamism, progress, and continuity; to its critics, however, Venice signals the state’s willingness to focus on the intangible elements of nationhood, namely culture and the arts. These critics use the ambiguities of the Venice rhetoric to legitimate their own appeals for change, especially after discovering that the “shared vision” of Venice is mainly in economic terms. By so doing, detractors of the state contest the centrality of economics in the making of modern—and future—Singapore, rendering the use of “Venice” as an image to promote the concept of a Global City problematic.     

ANALYZING EXPLOITATION

The codes of conduct of Western brand-name corporations normally require supplier factories in the Global South to comply with the local country’s minimum legal wage; the codes also often stipulate a maximum sixty-hour work week. But the problems of illegally low wages and overtime violations in supplier factories remain unresolved. This article uses survey data collected in a city in South China on workers’ wages and work hours to show how legal minimum wage rates, which normally are expressed in developing countries as a monthly wage, obfuscate the level of wages paid to workers. This will be demonstrated by comparing two different payment systems: time rates (which predominate in the toy industry) and piece rates (which predominate in garment manufacturing). The differences in the compensation rates and work hours resulting from the two systems lead the authors to contend that countries in the Global South and the implementers of corporate codes should calculate minimum wages in terms of hourly earnings in order to make wage payments more transparent and help reduce exploitative practices.     

Towards an integrated model of corruption: opportunities and control in China

This article uses a transaction costs approach to synthesize micro- and macro-theories of corruption in an analysis of corruption in post-Mao China. Macro-theories of corruption include functional, political-institutional, revised modernization, and cultural approaches. These approaches are integrated through a micro-model developed from the principal-agent model and a rational choice model of crime. The micro-model focuses on: the frequency of corrupt opportunities and the stakes involved, external control such as legal sanctions, and internal control or moral constraints. Within this context, this study reveals that corruption during the post-Mao China period was caused by the surge of such opportunities and the failure of internal and external control.     

鸡毒支原体醛缩酶的原核表达及膜定位鉴定

为了探明醛缩酶在支原体中的定位,根据已发表的鸡毒支原体醛缩酶(fba)基因序列设计特异性的引物,以鸡毒支原体Rlow株基因组为模板,通过Overlap PCR点突变扩增鸡毒支原体醛缩酶fba基因,将fba克隆至pET-28a(+)载体后进行序列测定和分析。结果表明,fba基因全长873bp,编码290个氨基酸。将构建的重组表达质粒pET28a-fba转化至大肠杆菌BL21(DE3),在IPTG诱导下成功获得表达融合蛋白rMGfba,大小约为33ku。纯化蛋白并对蛋白进行酶活性检测,结果显示该酶在体外具有与阳性对照醛缩酶相同的催化功能。用该蛋白免疫小鼠制备多克隆抗体,提取鸡毒支原体膜蛋白,Western-blot分析结果显示FBA多抗可与疏水性膜蛋白特异性结合,说明FBA位于鸡毒支原体膜表面。