Modified Midi‐ and Mini‐Multiplex PCR Systems for Mitochondrial DNA Control Region Sequence Analysis in Degraded Samples

Two multiplex polymerase chain reaction (PCR) systems (Midiplex and Miniplex) were developed for the amplification of the mitochondrial DNA (mtDNA) control region, and the efficiencies of the multiplexes for amplifying degraded DNA were validated using old skeletal remains. The Midiplex system consisted of two multiplex PCRs to amplify six overlapping amplicons ranging in length from 227 to 267 bp. The Miniplex system consisted of three multiplex PCRs to amplify 10 overlapping short amplicons ranging in length from 142 to 185 bp. Most mtDNA control region sequences of several 60‐year‐old and 400–500‐year‐old skeletal remains were successfully obtained using both PCR systems and consistent with those previously obtained by monoplex amplification. The multiplex system consisting of smaller amplicons is effective for mtDNA sequence analyses of ancient and forensic degraded samples, saving time, cost, and the amount of DNA sample consumed during analysis.     

Differentials in overall and cause-specific mortality among infants born in and out of wedlock,Stockholm 1878–1925

This study investigates differentials in the decline of cause-specific infant mortality by marital status of the mother in Stockholm (1878–1925) and factors contributing to the explanation of these differentials using computerized records of individual entries from the Roteman Archives. Included in the analysis were 120,094 children less than 1 year of age who lived in Södermalm during this period. Cause-specific mortality rates were calculated for three time periods. Cox's regression analysis was used to study the relationship between overall and cause-specific risk of infant death and of being born in and out of wedlock in relation to a set of variables. Infant mortality rates and mortality risks were higher among children born out of rather than in wedlock. The most pronounced differentials in cause-specific mortality rates between these groups of children were seen in cases of diarrhea. The socioeconomic status of the household head and number of children in the household were statistically significant with infant mortality, but explain only part of the excess mortality risk of children born out of wedlock. In Stockholm at the turn of the 19th century being born out of wedlock was strongly associated with poor health outcomes, particularly in diarrheal diseases, pneumonia/bronchitis, and immaturity/congenital causes.     

Predicting adult approval of corporal punishment from childhood parenting experiences

This study explores the relationship between mothers' approval of corporal punishment and the degree to which they themselves were subjected to violence as children. Considered as additional contributing factors are: (1) whether the mothers as children were punished by their own parents, (2) whether they perceived such punishment as unfair, and (3) the degree of parental nurture they experienced as children. The sample consisted of 330 new mothers whose mother and father both lived in the home when they were 14 years of age. Respondents were interviewed at home one to two months following their infants' discharge from the hospital. After controlling for race and income, no relationship was found between approval of corporal punishment and the violence to which mothers were subjected as children. However, significant associations were found between such approval and: (1) whether mothers were punished by their parents, and (2) maternal (but not paternal) nurture. Perceptions that parental punishment was unfair failed to contribute to such approval. Altogether, parental factors in mothers' childhoods, excluding race and income, accounted for 8.9% of the variance in approval of corporal punishment.     

Do lesser pleas pay?: Accommodations in the sentencing and parole processes

One of the important questions in the discussion of plea negotiation is whether a person who has received a labeling break by exchanging a plea of guilty for a reduced charge, really benefits in terms of the sentence he receives or the time he serves. The present study analyzes differences in sentence lengths or in actual periods of incarceration according to such variables as original charge, type of plea, conviction charge, and magnitude of charge reductions.The study shows that charge reduction may have pay-offs to the extent of directly reducing the maximum sentence possible and indirectly reducing the actual amount of time served. There are indications, however, that the parole process tends to neutralize the sentence differentials associated with charge reduction. The ratio between the time served and the sentence imposed tends to get higher as the magnitude of charge reduction increases.     

Allele frequencies and haplotypes of six new Y-specific STR loci in Koreans

Six Y-specific STR loci, DYS434, DYS435, DYS436, DYS437, DYS438, and DYS439 were analyzed in 300 unrelated Korean males using single multiplex PCR system.     

Implementation of the Routine Use Clause of the Privacy Act

Information privacy, the privacy of personally identifiable data held by organizations, gained access to the federal policy agenda in the sixties. The passage of several legislative acts resulted in protection for information privacy in specific environments and circumstances. In 1974, the Privacy Act was passed, providing citizens limited protection for data held by agencies of the federal government. While the Privacy Act is the cornerstone of protection for citizens with regard to government-held data, the law has been less effective than its designers hoped it would be. One major deject of the law is the routine use clause, which has been subjected to broad interpretation and distortion, to the benefit of executive branch agencies which handle personally identifiable data. This article examines the issue of information privacy, with focus on the misuse of the routine use clause and subsequent implications for citizen privacy.