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71.
Typing of mitochondrial DNA coding region SNPs of forensic and anthropological interest using SNaPshot minisequencing 总被引:10,自引:0,他引:10
Quintáns B Alvarez-Iglesias V Salas A Phillips C Lareu MV Carracedo A 《Forensic science international》2004,140(2-3):251-257
The development of new methodologies for high-throughput SNP analysis is one of the most stimulating areas in genetic research. Here, we describe a rapid and robust assay to simultaneously genotype 17 mitochondrial DNA (mtDNA) coding region SNPs by minisequencing using SNaPshot. SNaPshot is a methodology based on a single base extension of an unlabeled oligonucleotide with labeled dideoxy terminators. The set of SNPs implemented in this multiplexed SNaPshot reaction allow us to allocate common mitochondrial West Eurasian haplotypes into their corresponding branch in the mtDNA skeleton, with special focus on those haplogroups lacking unambiguous diagnostic positions in the first and second hypervariable regions (HVS-I/II; by far, the most common segments analyzed by sequencing). Particularly interesting is the set of SNPs that subdivide haplogroup H; the most frequent haplogroup in Europe (40-50%) and one of the most poorly characterized phylogenetically in the HVS-I/II region. In addition, the polymorphic positions selected for this multiplex reaction increase considerably the discrimination power of current mitochondrial analysis in the forensic field and can also be used as a rapid screening tool prior to full sequencing analysis. The method has been validated in a sample of 266 individuals and shows high accuracy and robustness avoiding both the use of alternative time-consuming classical strategies (i.e. RFLP typing) and the need for high quantities of DNA template. 相似文献
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Gusmão L Sánchez-Diz P Alves C Quintáns B García-Poveda E Geada H Raimondi E Silva de la Fuente SM Vide MC Whittle MR Zarrabeitia MT Carvalho M Negreiros V Prieto Solla L Riancho JA Campos-Sánchez R Vieira-Silva C Toscanini U Amorim A Carracedo A;GEP-ISFG 《Forensic science international》2003,135(2):150-157
The Spanish and Portuguese ISFG Working Group (GEP-ISFG) carried out a collaborative exercise in order to asses the performance of two Y chromosome STR tetraplexes, which include the loci DYS461, GATA C4, DYS437 and DYS438 (GEPY I), and DYS460, GATA A10, GATA H4 and DYS439 (GEPY II). The groups that reported correct results in all the systems were also asked to analyse a population sample in order to evaluate the informative content of these STRs in different populations. A total of 1020 males out of 13 population samples from Argentina, Brazil, Costa Rica, Macao, Mozambique, Portugal and Spain were analysed for all the loci included in the present study. Haplotype and allele frequencies of these eight Y-STRs were estimated in all samples. The lowest haplotype diversity was found in the Lara (Argentina) population (95.44%) and the highest (99.90%) in Macao (China). Pairwise haplotype analysis showed the relative homogeneity of the Iberian origin samples, in accordance with what was previously found in the European populations for other Y-STR haplotypes (http://www.ystr.org). As expected, the four non-Caucasian samples, Macao (Chinese), Mozambique (Africans), Costa Rica (Africans) and Argentina (Lara, Amerindians), show highly significant Phist values in the pairwise comparisons with all the Caucasian samples. 相似文献
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The special issue on the emergence and maintenance of depression and depressive symptoms is introduced. The special issue considers two typically separate lines of research, one focusing on severe clinical depression and another on depressive symptoms. The biological, social, and cognitive factors contributing to the emergence of depression in adolescence are highlighted in this special issue.Received Ph.D. from the University of Pennsylvania. Research interests include biological and social development of adolescents and changes in development over a series of life cycle transitions.Received Ph.D. from the University of Chicago. Research interests include biopsychosocial aspects of adoleslcent development. 相似文献
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Billie Jeanne Brownlee 《Third world quarterly》2017,38(10):2276-2294
This article intends to provide responses to some of the many unanswered questions about the making and the transformation of the uprising in Syria by exploring a new avenue of research: media development aid. Most academic interest has been oriented towards the role that the new media played at the time of the uprising; insufficient interest, by contrast, has been directed to the development of the sector in the years predating it. What emerges from this article is that the Syrian media landscape was strongly supported by international development aid during the years prior to the outbreak of the uprising of 2011. By looking at the complex structure of media aid architecture and investigating the practices and programmes implemented by some representative organisations, this article reflects on the field of media development as a new modus operandi of the West (the EU and US especially), to promote democracy through alternative and non-collateral, bottom-up support. 相似文献
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This commentary uses the contributions of the 4-H Study of Positive Youth Development as a chance to reflect on the progress of the past two decades and suggest important tasks for the future. Meaningful advances in theory now set the stage for needed work on measurement, evaluation, and understanding of contextual processes. The commentary ends with a plea for an integration of the research and programmatic approaches of promotion and prevention in order to study youth development and implement youth programs. 相似文献
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Few studies on the correlates of school violence include school and neighborhood influences. We use ecological systems theory and social disorganization theory to simultaneously incorporate neighborhood (e.g., concentrated poverty, residential instability, and immigrant concentration), school, family, and individual predictors of physical school victimization longitudinally among a large socio-economically and ethnically diverse (49 % Hispanic; 34 % African American) sample of 6 and 9 year olds (49 % female) from the Project on Human Development in Chicago Neighborhoods. These children were followed up at Wave II at ages 8 and 11 (n = 1,425). Results of Hierarchical Generalized Linear Models reveal neighborhood residential instability increases school victimization net of family and individual correlates. Furthermore, cross-level interactions were also supported where residential family mobility has a stronger risk influence in areas of high residential instability. Also, the influence of residential family mobility is decreased in areas with higher levels of immigrant concentration. We also found cross-context connections where parent-to-child aggression in the home is connected to a higher risk of victimization at school. The role of neighborhood and family residential instability on victimization warrants further research. 相似文献