Results of the 2018 Rapid DNA Maturity Assessment

Three commercially available integrated rapid DNA instruments were tested as a part of a rapid DNA maturity assessment in July of 2018. The assessment was conducted with sets of blinded single-source reference samples provided to participants for testing on the individual rapid platforms within their laboratories. The data were returned to the National Institute of Standards and Technology (NIST) for review and analysis. Both FBI-defined automated review (Rapid DNA Analysis) and manual review (Modified Rapid DNA Analysis) of the datasets were conducted to assess the success of genotyping the 20 Combined DNA Index System (CODIS) core STR loci and full profiles generated by the instruments. Genotype results from the multiple platforms, participating laboratories, and STR typing chemistries were combined into a single analysis. The Rapid DNA Analysis resulted in a success rate of 80% for full profiles (85% for the 20 CODIS core loci) with automated analysis. Modified Rapid DNA Analysis resulted in a success rate of 90% for both the CODIS 20 core loci and full profiles (all attempted loci per chemistry). An analysis of the peak height ratios demonstrated that 95% of all heterozygous alleles were above 59% heterozygote balance. For base-pair sizing precision, the precision was below the standard 0.5 bp deviation for both the ANDE 6C System and the RapidHIT 200.     

Measuring Neoliberalism: Development and Initial Validation of a Scale of Anti-Neoliberal Attitudes

Critics of neoliberalism argue that so-called meritocratic and identity-neutral social policies and political positions actually reinforce and exacerbate intersecting inequalities, namely racism, sexism, heterosexism, classism, and ethnocentrism/xenophobia. The purpose of these studies was to develop and initially validate a scale of neoliberal attitudes from a wide range of existing instruments that reflect anti-neoliberal theory. A series of three studies resulted in a 25-item instrument—the Anti-Neoliberal Attitudes Scale (ANAS)—that exhibits initial evidence of construct validity, internal consistency, and test–retest reliability. Exploratory factor analysis with students from two universities revealed a four-factor structure of Racism and Sexism Awareness, Communitarian Values, Multicultural Ideology, and Inequality Consciousness. However, a confirmatory factor analysis with an independent sample of undergraduate students suggests a bifactor model in which the general factor explains most of the variance and that the instrument should be treated as a single scale, rather than independent subscales. Significant correlations with measures of right-wing authoritarianism and social dominance orientation suggest convergent validity. Temporal stability was established via a test–retest analysis in an independent sample of undergraduate students. Finally, responses from a sample of MTurk workers provided evidence of the ANAS’s incremental validity when compared to an existing measure of neoliberal beliefs. Implications for future empirical work on the psychological dimensions of neoliberalism are discussed.

     

Dimensions of Conscience in Mid-adolescence: Links with Social Behavior, Parenting, and Temperament

The purpose of this study was to determine whether aspects of conscience cohere into broader dimensions and to examine how these broader dimensions of conscience relate to parenting, temperament, and social behavior. One hundred and thirteen adolescents (M age = 15.88 years, 51% female) completed measures of sympathy, guilt, empathic anger, shame, moral reasoning, and internalization. Factor analyses revealed two dimensions of conscience: moral affect (which included guilt, shame, sympathy, and empathic anger) and moral cognition (which included internalization and prosocial moral reasoning). Parental discipline related to both dimensions of conscience, albeit in different ways. High levels of parental inductive discipline and low levels of parental power assertion were associated with high levels of moral affect, whereas high levels of persistent discipline were associated with increased moral cognition. High negative reactivity, however, was only associated with high levels of moral affect. In general, higher levels of moral affect were associated with prosocial behavior and moral conduct during bullying. Higher levels of moral cognition were associated with less participation in bullying, more altruistic behavior, and more frequent helping of the victims of bullying. The discussion focuses on the dimensions of moral conscience in adolescence.

An innovative approach to building stronger coalitions: the Net-Map Toolbox

A common challenge faces development organisations, from the highest policy-making circles to local, grassroots organisations: how to work with other groups to build stronger partnerships and achieve consensus on goals? This article describes the Net-Map Toolbox, a new tool which builds and expands upon existing social-networking approaches. The article highlights the experience of using the Toolbox with the White Volta Basin Board in Ghana, a multi-stakeholder organisation responsible for overseeing local water resources. The authors discuss how the Net-Map Toolbox can assist members of development-oriented organisations to better understand and interact with each other in situations where many different actors can influence the outcome.     

Analysis of forensic SNPs in the canine mtDNA HV1 mutational hotspot region

A 60 bp sequence variation hotspot in the canine mitochondrial DNA hypervariable region 1 was evaluated for its use in forensic investigations. Nineteen haplotypes containing 18 single nucleotide polymorphisms were observed among laboratory-generated and GenBank-derived domestic dog sequences representing five regional localities in the U.S. Samples from the different localities were highly variable with the levels of intra-population variability being similar among the populations studied. AMOVA further confirmed that there was no significant genetic structuring of the populations. Assays using these haplotypes were robust, canid specific and portend a rapid method for correctly excluding individual dogs as noncontributors of forensic evidence. Species-specificity of the primers was confirmed by means of in-tube polymerase chain reaction of human and cat DNA and in-silico assessment of the genomes of several animal species. Breed-specific fragments were not detected among the common haplotypes but there is evidence that this assay may be capable of differentiating domestic dog, wolf, and coyote sequences.     

Human subjects protections in biomedical enhancement research: assessing risk and benefit and obtaining informed consent

The protection of human subjects in biomedical research relies on two principal mechanisms: assessing and comparing the risks and potential benefits of proposed research, and obtaining potential subjects' informed consent. While these have been discussed extensively in the literature, no attention has been paid to whether the processes should be different when the objective of an experimental biomedical intervention is to improve individual appearance, performance, or capability ("enhancement research") rather than to prevent, cure, or mitigate disease ("health-oriented research"). This essay examines this question in order to ensure that subjects in biomedical enhancement research receive adequate protection.     

Student Age and the Collegiate Pathway

Using a rich data set of all SAT test takers from the 2004 through 2008 high school graduation cohorts, we investigate the impact of state‐specific school age‐of‐entry laws on students’ pathways into and through college. We document that these laws do not impact the probability that a student takes the SAT; however, we find strong evidence that students who are expected to be the oldest in their school cohorts based on their state residency and birthdays have a greater probability of taking an Advanced Placement (AP) exam and tend to take more AP exams. We also find that relatively younger students are more likely to attend two‐year colleges before attending four‐year colleges and are less likely to have earned bachelor's degrees four years beyond high school graduation, but eventually catch up to their older peers six years beyond high school graduation.