Increasing Allele Detection by Altering the Quantity of Internal Lane Standard

Electrokinetic injection (EI) is the primary method used in forensic laboratories to load amplified PCR product in capillary electrophoresis for short tandem repeat (STR) fragment separation. Because all samples subjected to capillary electrophoresis use internal lane standard (ILS), this study investigated the consequence of varying the volume of ILS and its effects on allele peak heights and number of alleles detected. Results demonstrated that when the volume of ILS is reduced, the average peak height and number of alleles increased, thereby increasing the sensitivity of the detection method. Sizing anomalies were observed; however, they did not adversely affect accuracy and precision. The method developed in this study offers a simple and universal procedure to increase the alleles detected in forensic STR analysis. Reducing the volume of ILS to achieve greater sensitivity is applicable to all STR amplification kits and capillary electrophoresis instruments currently used in forensic DNA analysis.     

An Update on the Hazards and Risks of Forensic Anthropology,Part II: Field and Laboratory Considerations

This paper focuses on potential hazards and risks to forensic anthropologists while working in the field and laboratory in North America. Much has changed since Galloway and Snodgrass published their seminal article addressing these issues. The increased number of forensic practitioners combined with new information about potential hazards calls for an updated review of these pathogens and chemicals. Discussion of pathogen hazards (Brucella, Borrelia burgdorferi, Yersinia pestis, Clostridium tetani and West Nile virus) includes important history, exposure routes, environmental survivability, early symptoms, treatments with corresponding morbidity and mortality rates, and decontamination measures. Additionally, data pertaining to the use of formaldehyde in the laboratory environment have resulted in updated safety regulations, and these are highlighted. These data should inform field and laboratory protocols. The hazards of working directly with human remains are discussed in a companion article, “An Update on the Hazards and Risks of Forensic Anthropology, Part I: Human Remains.”     

Civil Society as Ideology in the Middle East: A Critical Perspective

Since the early 1990s support for civil society has constituted the linchpin of international efforts in the Middle East North Africa (MENA) to promote democratisation and democratic values. The rationale for this support lies in an understanding of civil society drawn from a liberal-democratic model, which dominates debates about civil society. This paper highlights the inaccuracies of this model when applied to the MENA and, using Turkey as an example, draws attention to the perils of supporting civil society organisations (CSOs) based on its conjectures. A critical analysis of CSOs and their role in Turkish society, drawing on the theoretical framework laid down by Gramsci, highlights two key issues: (1) contrary to the dominant policy view which equates civil society organisations with democracy, CSOs often assist elites in both democratic and undemocratic states to extend and consolidate their political economic power; (2) the idealisation of civil society by Western policy makers results in a diminished awareness of the factors which weaken civil society and erodes its democratic potential. Overall, the findings support the assertion that CSOs in the MENA facilitate predominantly elite interests over those of ordinary citizens and democracy more broadly.     

The Admissibility of the R-PAS in Court

The admissibility of the Rorschach has been a concern of forensic psychologists for many years. The focus of this debate has been the Comprehensive System, which is the most researched of the current Rorschach Systems available in the USA. However, recently, a new, competing system has been published: the Rorschach Performance Assessment System (R-PAS; Meyer et al., Rorschach Performance Assessment System: Administration, Coding, Interpretation, and Technical Manual, 2011). Using Heilbrun’s (Law and Human Behavior 16:257–272, 1992) guidelines as a framework, we examine the admissibility of this new system according to the standards outlined in Daubert (1993) and Frye (1923). We conclude that we have reservations about the admissibility of the R-PAS in court at the present time, notwithstanding ongoing work on this system.     

Sustainability of the effects of multisystemic therapy for juvenile delinquents in The Netherlands: effects on delinquency and recidivism

Objectives

The present study focused on the sustainability of the effects of Multisystemic Therapy (MST) on delinquency and recidivism.

Methods

A sample of 256 juveniles with severe and persistent antisocial behavior were randomly assigned to MST (147) and Treatment As Usual (TAU) (109) condition. Pre-test assessment took place before the start of MST/control group treatment. Post-test assessment took place at 6 months after termination of the program. Delinquency (parent and adolescent reported) was assessed 6 months after termination of the treatment. Official judicial data were collected to assess recidivism, with a mean length of follow-up of 3.06 years. ANCOVAs and survival analyses were used to test the effectiveness of MST.

Results

The multi-informant data showed that MST is effective in diminishing delinquent behavior as reported by adolescents and parents, with d’s larger than at post-test assessment immediately after ending of the intervention. The official judicial data, however, suggest that there are no differences between MST and TAU in recidivism. Few and inconsistent moderator effects were found.

Conclusions

According to parent and adolescent reports, the beneficial effects of MST were sustained at the follow-up. This was not supported by official data. These results stress the importance of using multi-informant data on delinquency, as each source of information has its own advantages and disadvantages.     

Arts and Cultural Engagement,Reportedly Antisocial or Criminalized Behaviors,and Potential Mediators in Two Longitudinal Cohorts of Adolescents

Journal of Youth and Adolescence - Arts and cultural engagement is a potential strategy for reducing or preventing reportedly antisocial or criminalized behaviors (those previously and...     

Meaning in Life Moderates Relations between Personality and Temperament and Nonsuicidal Self-Injury in Hospitalized Adolescents

Journal of Youth and Adolescence - Previous research indicates that sensation seeking, emotion dysregulation, and impulsivity are predictive of non-suicidal self-injury (NSSI). A body of research...     

Development of a Questionnaire on Intentions to Engage in Dating Violence Among Puerto Rican Residents

Journal of Family Violence - This mixed-methods paper describes the development and preliminary validation of the Behavioral Intentions Questionnaire (BIQ), a multi-scale questionnaire developed to...     

Host‐habitat Location by the Parasitoid,Nasonia vitripennis Walker (Hymenoptera: Pteromalidae)

This study investigated the role of odorant cues used during host‐habitat location by the generalist parasitoid, Nasonia vitripennis Walker. Nasonia vitripennis is a common parasitoid of Dipteran pupae found in association with decaying carrion. Behavioral assays were used to investigate the host‐habitat searching behavior under different scenarios. First, we demonstrated N. vitripennis to be significantly attracted toward odorant cues associated with decaying meat. The biological activity of nine of the volatile molecules constituting the odor of decaying meat was tested on the searching behavior of parasitoid females through two complementary chemoecological approaches: electroantennography (EAG) and olfactometry bioassays. Butanoic acid and butan‐1‐ol elicited high olfactory responses, but no attraction was induced by these two chemicals. Behavioral assays showed that, among the VOCs tested, methyldisulfanylmethane (DMDS) was the only volatile chemical to induce attraction in N. vitripennis.