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In this work, a number of performance checks were carried out to evaluate the efficacy of commercial Y-short tandem repeats (Y-STR) kits for casework applications. The study evaluated the sensitivity, specificity and stability of the Y-STR markers used and the ability to obtain a male profile from postcoital samples taken at various time points after intercourse. All systems performed well with 1-3 ng of male DNA as recommended by the manufacturers. All systems gave full profiles at 100 pg of input DNA, which is within the realm of low copy number DNA analysis. Moreover all, except Y-Plex12, gave full profiles with 30-50 pg of male DNA. No increased performance was obtained with any of the systems by increasing the cycle number beyond that recommended by the various manufacturers. When up to 1 microg of female DNA was used (in the absence of male DNA) no female DNA cross reactivity was observed with the Y-Plex 12 and Y-Filer systems. PowerPlex Y produced female DNA derived products near the DYS438 and within the DYS392 loci at a rare allele position with high input DNA levels (300 ng and 1 microg, respectively). Male/female DNA admixture experiments indicated the particularly high specificity of the Y-Filer and PowerPlex Y systems under conditions of several thousand fold female DNA excess. All systems were able to detect the minor alleles in male/male DNA admixtures at a 1:5 dilution with the PowerPlex Y and Y-Filer being able to detect some minor alleles at 1:20. Species testing indicated some limited, minor cross reactivity of the commercial systems with some domestic male mammals although it is easily recognizable and would not pose any problems in casework analysis. As expected a significant number of cross-reacting products were obtained with nonhuman primate species. All Y-STR multiplex systems tested were able to produce complete Y-STR profiles from bloodstains and semen stains exposed up to 6 weeks when the samples were protected against precipitation and sunlight. However, exposure of the samples to precipitation either in the presence or absence of sunlight resulted in Y-STR profile loss over time, with total profile loss occurring with all systems after 3 weeks or more. Complete Y-STR profiles of the male donors up to 72 h postcoitus were obtained with all of the multiplex systems tested, except for Y-Plex12, which gave partial profiles. 相似文献
175.
Heide KM Boots DP 《International journal of offender therapy and comparative criminology》2007,51(6):646-675
This article is a content analysis of 150 unique cases of children killing parents in the United States as reported in the electronic news media. The accuracy of online coverage of U.S. parricide incidents is assessed using two types of resources: officially reported national statistics on known parricidal incidents and the psychological and psychiatric literature on matricide and patricide. Comparisons of news accounts of media-reported U.S. parricide cases with Supplementary Homicide Report data indicate that electronic media coverage of parricide cases focused on the more sensational and unusual parricides. Analyses of these media accounts by offender age found 13 significant differences between juvenile and adult offenders. Ten of these 13 differences related to motive and Heide's parricide offender types (severely abused, severely mentally ill, and dangerously antisocial) and were consistent with the mental health-related literature in this area. The limitations and directions for future research are discussed at length. 相似文献
176.
This study examines the effects of perceptions of social disorganization on crime perpetration and victimization among a sample of incarcerated adult gang and non-gang members. Using survey data from 2,414 jail inmates, results suggest that gang members are significantly more likely than non-gang members to be both perpetrators and victims of property and personal crimes. Results also indicate substantive similarities and differences between gang members and non-gang members with regard to crime, victimization, and perceptions of social disorganization. Inmates’ perceptions of the level of social disorganization in their neighborhoods are more strongly related to their reported offending behavior than to their reported victimization. 相似文献
177.
Few criminological theories have been applied to the study of stalking perpetration, and even fewer address the presence of underlying psychological mechanisms. Attachment theory describes the ways in which an individual with a chaotic family environment in childhood may develop feelings of insecurity that may lead to increased aggression and violent behavior in adolescence and adulthood. In this study, a sample of college students (N = 2,783) were queried on self-reported stalking behaviors and the revised Experiences in Close Relationships (ECR-R) measure of adult attachment. Stalkers scored significantly higher on the insecure-anxious scale of attachment and lower on insecure-avoidant scale. Other psychological variables (major/minor psychiatric diagnoses, depression, and anger-related issues) were also examined, with a history of anger-related diagnosis or treatment positively and significantly associated with stalking perpetration. Implications for theory development in stalking and future research directions are discussed. 相似文献
178.
Marco Aurelio Guimarães José Arnaldo Soares-Vieira Ricardo Henrique Alves da Silva Martin Paul Evison 《Forensic Science International: Genetics Supplement Series》2009,2(1):165-166
At the Medical Legal Center in Ribeirão Preto, Brazil (CEMEL/FMRP-USP), unidentified decomposing bodies routinely undergo soft tissue removal (by immersion in water at 80–90 °C for 24 h) prior to an anthropological analysis intended to yield a biological profile of age, sex, ancestry, height, pathology and so on. In the event that this analysis is unsuccessful, samples may be submitted for DNA profiling. The tropical climate and the defleshing process may confound preservation, recovery and analysis of DNA, however. In order to establish an optimal standardized protocol for identification of decomposing human remains from a tropical climatic region, the outcome of anthropological and genetic analyses was compared, along with the utility of bone (mainly femur and sternum) and teeth (mainly molar) specimens for DNA analysis. In a sample (n = 39) of partially skeletonized remains, anthropological analysis was sufficient for identification in eight cases. In further six cases, DNA profiling was successfully attempted. As a consequence of our study, we recommend collection of 1–2 well preserved teeth prior to defleshing and anthropological analysis in these circumstances. 相似文献
179.
Kathleen Ramirez 《Family Court Review》2019,57(2):258-272
The need to protect public health has increased as the anti‐vaccine movement is on the rise. Exemptions to vaccination requirements have become more lax, and parents find ways to avoid vaccinating their children, thereby exposing not only their children to the dangers of serious diseases, but other children as well. This Note proposes a federal statute mandating that parents subject their children between the ages of infancy and 6 to acquire vaccinations for all diseases recommended by the Centers for Disease Control and Prevention unless a licensed physician recommends otherwise. 相似文献
180.
An Objective Measure of Splitting in Parental Alienation: The Parental Acceptance–Rejection Questionnaire 下载免费PDF全文
William Bernet M.D. Nilgun Gregory Ph.D. Kathleen M. Reay Ph.D. Ronald P. Rohner Ph.D. 《Journal of forensic sciences》2018,63(3):776-783
Both clinicians and forensic practitioners should distinguish parental alienation (rejection of a parent without legitimate justification) from other reasons for contact refusal. Alienated children—who were not abused—often engage in splitting and lack ambivalence with respect to the rejected parent; children who were maltreated usually perceive the abusive parent in an ambivalent manner. The purpose of this study was to assess the usefulness of the Parental Acceptance–Rejection Questionnaire (PARQ) in identifying and quantifying the degree of splitting, which may assist in diagnosing parental alienation. Results showed that severely alienated children engaged in a high level of splitting, by perceiving the preferred parent in extremely positive terms and the rejected parent in extremely negative terms. Splitting was not manifested by the children in other family groups. The PARQ may be useful for both clinicians and forensic practitioners in evaluating children of divorced parents when there is a concern about the possible diagnosis of parental alienation. 相似文献