Evaluation of Commercial Kits for Dual Extraction of DNA and RNA from Human Body Fluids, ,

STR typing of DNA evidence can identify the donor with a high power of discrimination but cannot identify the tissue origin of a body‐fluid stain. Using RNA to attribute a crime scene stain to a particular tissue may aid in reconstruction efforts. With blood from 10 donors, four DNA and RNA coextraction kits were evaluated by measuring yields and STR and mRNA profiles. T tests indicated some significant differences in kit performance. The Zymo Research ZR‐Duet^? kit performed best based on average DNA (41.4 ng) and mRNA (4.07 ng) yields and was the only kit to provide complete DNA/RNA profiles for all samples. The consistency of this kit was challenged by data from additional blood and saliva donors. Further testing is advised before a superior kit is unequivocally chosen. Stand‐alone DNA or RNA purification generally offers higher yield, but coextraction may still allow successful STR profiling and tissue source identification.     

Family influences,school behaviors,and risk for the later development of an eating disorder

Interviews were conducted with parents of 136 female and 45 male adolescents categorized into risk groups for the later development of an eating disorder. The family and school concomitants of risk status in females were demonstrated to be different from that in males. Risk group female adolescents rated family cohesion, parent-adolescent communication processes, and overall family satisfaction more negatively than the comparison group. Mothers of moderate risk group females reported lower family cohesion than the comparison group; there were no group differences for adolescent females in fathers' ratings of family measures. However, no group differences were found on any of the family measures between male risk and comparison males. For both females and males, there were no significant group differences in family history of eating and mood disorders, or alcohol dependence. Teacher ratings indicated relatively greater internalizing tendencies in the high-risk female group.This investigation was supported by NICHD Grant Number 1R01-HD24700 awarded to Gloria R. Leon.Received Ph.D. from University at Maryla. Research interests include precursors of eating disorders and stress and coping in extreme environments. To whom correspondence should be addressed.Received M.A. from San Diego State University. Research interests include precursors of eating disorders, substance abuse, and personality.Received Ph.D. from Stanford University. Research interests include psychosocial aspects of health promotion and disease prevention.Received B.A. from University of Maine. Research interests life span development and family issues.     

Adolescents' perceptions of the costs and benefits associated with cigarette smoking: Sex differences and peer influence

This study explored the perceptions of young adolescents of the costs and benefits of cigarette smoking. These perceptions were examined as a function of the sex of the adolescent and peer smoking habits. The sample consisted of 155 White middle class male and female adolescents, aged 12 to 15. The results indicate that endorsement of particular costs and benefits was related to the respondent's sex and whether or not the respondent had friends who smoked. The girls seemed to view smoking as a sign of rebellion or autonomy, while the boys seemed to view smoking cigarettes as a social coping mechanism. The effect of having friends who smoke was always mediated by the sex of the adolescents. Boys who have friends who smoke have attitudes that appear more conducive to smoking than do boys with nonsmoking friends. This relationship did not hold for girls. The implications for smoking education and intervention are discussed.This work was supported in part by the Comprehensive Cancer Center of Metropolitan Detroit as supported by Grant CA - 22453 from the National Cancer Institute, DHHS.     

Child Maltreatment and the Acquisition of Identity Capital: A Retrospective Exploration of a Transformative Social Justice Experience

Abstract

The purpose of this qualitative research study was to explore whether secondary classes foster the acquisition of certain agentic capacities for formerly maltreated adolescents. We utilized the identity capital model (ICM) to explore the identity capital acquired by a socioeconomically diverse group of 40 Black former students in a mandatory, high school transformative social justice (TSJ) class. All participants disclosed some form of child maltreatment. The most prominent IC acquired for all participants included purpose, social perspective taking, moral-ethical reasoning, and resilience. Implications are discussed.     

Interpersonal violence in adolescence: ecological correlates of self-reported perpetration

Although growing attention is being paid to the problem of teen dating violence, to date less is known about perpetrators of victimization. The current article used a subset of 980 adolescents aged 11 to 19 who were surveyed as part of a statewide community service coordinated through Cooperative Extension to survey all youth in target communities about risky health behaviors. The current article examined correlates of perpetration of either physical dating violence or sexual abuse across all levels of the ecological model (individual, family, and community factors). At the bivariate level, individual factors including substance use and low social responsibility, family factors including divorce, low parental monitoring, and low social support, and community variables such as low school attachment and neighborhood monitoring were associated with self-reported perpetration. At the multivariate level, gender and history of victimization were most significant in explaining variance in perpetration.     

Courtship violence using couple data: characteristics and perceptions

This study examined courtship violence using couple data. Participants were 50 heterosexual dating couples with at least one member of the couple being a university student. Using the Conflict Tactics Scales 2 (CTS2), both members of a couple reported on received and inflicted physical and psychological violence. The prevalence of physical aggression depended on how violence was defined. On the CTS2, 60% of couples were considered physically violent when within-couple agreement was not considered; this dropped to as low as 28% when agreement had to be present. Women were more likely than men to report inflicting psychological aggression and to report aggressing against their partner with a playful intent. Taken together, these findings demonstrate the need to continue to examine courtship violence, especially by utilizing responses from both members of a couple.