Development of a human-specific real-time PCR assay for the simultaneous quantitation of total genomic and male DNA

A duplex real-time quantitative PCR assay was developed for forensic DNA analysis, which provides simultaneous quantitation of total genomic human DNA and human male DNA. The assay utilizes two spectrally resolved fluorogenic probes in a 5' nuclease (TaqMantrade mark) assay. Within the range of organisms empirically tested and based upon theoretical specificity using National Center for Biotechnology Information GenBank sequences, primer and probe sequences were shown to be human specific, and the Y-chromosome probe, male-specific. A mixture-challenge study resulted in accurate quantitation of 25 pg male DNA in a mixture of up to 1:5000 (male:female DNA). Additional experimental results include comparisons with the slot blot method and commercial real-time PCR kits. The assay developed addresses the shortcomings of the traditional slot blot method as well as the commercial real-time PCR kits. This method is shown to be specific, relatively simple, rapid, has low limits of detection, and consumes limited sample in addition to reporting both the male and total genomic DNA concentrations present.     

Beyond Liberalism: Marxist Feminism,Migrant Sex Work,and Labour Unfreedom

In this article, I use a Marxist feminist methodology to map the organisation of migrant sex workers’ socially reproductive paid and unpaid labour in one city and country of arrival, London, UK. I argue that unfree and ‘free’ (sexual) labour exists on a continuum of capitalist relations of (re)production, which are gendered, racialised, and legal. It is within these relations that various actors implement, and migrant sex workers contest, unfree labour practices not limited to the most extreme forms. My analysis reveals that many migrant sex workers have very limited ‘freedom’. This is in stark contrast to the classical liberal claim of sex worker rights activists and academics that the vast majority of migrant sex workers are free, and therefore not coerced, exploited or trafficked. I then consider whether the emerging labour approach to trafficking could help achieve ‘freedom’ for migrant sex workers. Advocates argue that anti-trafficking efforts must, and can, be refocused on extending minimum labour and social protections to all vulnerable workers. I argue that this approach is disconnected from material interests and history. Rather, migrant sex workers, sex worker rights activists, and all migrant and citizen workers and activists globally must collectively organise against ‘labour unfreedom’ and hence for meaningful control over their labour and lives.     

From Victimization to Criminalization: General Strain Theory and the Relationship Between Poly-victimization and Delinquency

Although it is well established that victimization and delinquency are related, it is not clear how this relationship works, and victims and offenders are still often studied and treated as two distinct groups. General strain theory views victimization as a form of injustice, which can give rise to anger and eventually lead to delinquency. The authors examine victimization, in particular polyvictimization, as a criminogenic factor. Based on a sample of 1,400 youths 12–17 years old in Quebec (Canada), they examine polyvictimization in relation to delinquency as well as negative emotions that can result from victimization namely anger, depression, and posttraumatic stress. The findings show support for general strain theory and highlight the importance of anger for the relationship between victimization and juvenile delinquency.     

Foreign Aid and Undeserved Credit Claiming

Politicians in developing countries misuse foreign aid to get reelected by fiscally manipulating foreign aid resources or domestic budgets. Our article suggests another mechanism that does not require politicians to have any control over foreign aid in order to make use of it for electoral purposes: undeserved credit claiming. We analyze the conditions under which local politicians can undeservedly take credit for the receipt of foreign aid and thereby boost their chances of reelection. We theorize that politicians can employ a variety of techniques to claim credit for development aid even when they have little or no influence on its actual allocation. Using a subnational World Bank development program in the Philippines, we demonstrate that credit claiming is an important strategy to exploit foreign aid inflows and that the political effects of aid can persist even when projects are designed to minimize the diversion or misuse of funds.     

Toner Particles as Forensic Evidence: Microanalytical Characterization of Known Toner and Recognition of Toner in Environmental Samples*†‡

Modern printing toners represent a prime example of subvisible particles that can be easily transferred to hands, clothing, and other surfaces. To explore the potential evidentiary value of toner particles, toner samples were collected from known printer cartridges and characterized by various microanalytical techniques to establish the properties most useful for recognition, identification, and comparison. Environmental samples (i.e., dust) were then collected from various locations at varying distances from toner-based printers, using both tape lifts and carbon adhesive stubs, to assess the possibility of detecting toner. By light microscopy, toner can be recognized on the basis of particle size and shape, as well as color. Further examination of the micromorphology in the field emission scanning electron microscope reveals characteristic morphologies and differences in surface texture and shape among toner sources. Raman spectroscopy provides chemical identification of the pigment (or pigment class) and, in some cases, also permits identification of the polymer component. While black and blue pigment chemistry remained constant among toner varieties that were studied (copper phthalocyanine and carbon black), variation in yellow and magenta pigments was observed. Analysis of dust samples collected from various environments demonstrated that while toner is consistently detectable in close proximity to printers (within 2 feet), it also can be detected in dust collected in nearby rooms. This research demonstrates that toner particles can be located, characterized, and discriminated, using a suite of microanalytical methods that are applicable to forensic casework.     

Baker & McKenzie's regular article tracking developments in EU law relating to IP, IT and telecommunications

This is the latest edition of Baker & McKenzie's column on developments in EU law relating to IP, IT and telecommunications. This article summarises recent developments that are considered important for practitioners, students and academics in a wide range of information technology, e-commerce, telecommunications and intellectual property areas. It cannot be exhaustive but intends to address the important points. This is a hard copy reference guide, but links to outside web sites are included where possible. No responsibility is assumed for the accuracy of information contained in these links.     

Direct carboxyhemoglobin determination by derivative spectroscopy

A fast and sensitive method for the determination of carboxyhemoglobin (COHb) in blood using derivative spectroscopy is described. The addition of sodium dithionite as a reducing agent is not required. The concentration interval studied was from 0.5% to 100% COHb.