A novel method for ABO genotyping using a DNA chip

ABO genotyping is often performed to identify the blood type of decomposed samples, which is difficult to be determined by a serological test. In this study, we developed a simple method for ABO genotyping using a DNA chip. In this method, polymerase chain reaction-amplified and fluorescent-labeled fragments in the ABO gene and primate-specific D17Z1 were hybridized with DNA probes on a chip designed to detect single nucleotide polymorphisms (SNPs) in the ABO gene and part of the D17Z1 sequence. Using blood samples from 42 volunteers and 10 animal species, we investigated whether the chip could be used to detect SNPs in the ABO gene and the D17Z1 sequence. This method was then applied to various forensic samples, and it was confirmed that this method was suitable for the simultaneous analyses of ABO genotyping and species identification. This method fulfills the recent need for the development of rapid and convenient methods for criminal investigations.     

Estimation of age from the femur of Japanese cadavers

The purpose of this study is to estimate the age of cadavers by histomorphometry of the femur. Seventy-two Japanese males ranged from 43 days to 92 years old and 26 females ranged from 2 to 88 years old were used. The thickness of sections was adjusted at 50 to 70 microns by grinding with sand paper. The sections were not decalcificated. They were stained with Villanueva's bone staining powder and with thionin dye. Microradiographs of the sections were obtained by the soft X-ray apparatus. The area, maximum and minimum diameter, and perimeter of the perfect osteon and Haversian canal were measured. In addition, the type II osteon number, osteon fragment number, and area of triangle were also determined. All these parameters were examined by an image analyzer. The parameters of the osteon showed high correlation coefficient with age (magnitude of r > 0.77), while those of the Haversian canal were low (magnitude of r < 0.11). All parameters were subjected to multiple regression analysis for producing a multiple regression equation of age estimation. For the stepwise selecting method, the perimeter of osteon, maximum length of the Haversian canal and osteon fragment number were selected for the equation. Their multiple r2 and standard error of estimation were 0.8874 and 6.39, respectively. For the forward selection method, in addition to the above items, three parameters, the maximum length of Haversian canal, triangle area, osteon fragment number were selected. Their multiple r2 and standard error of estimation were 0.9484 and 4.884, respectively. Bone staining was useful to clarify the demarcation between osteon and fragment, leading to an increase in the accuracy of age estimation. However, the entire range from birth to 90 years was difficult to cover for precise age estimation.     

An Autopsy Case of Acute Massive Hematochezia Caused by Superior Mesenteric Vein Thrombosis: A First Report in Forensic Medicine

Superior mesenteric vein thrombosis (SMVT) is an uncommon cause of intestinal ischemia and massive gastrointestinal bleeding. This report describes a man with alcoholic liver cirrhosis, who died of massive hematochezia due to SMVT. A medicolegal autopsy disclosed a thrombus at the superior mesenteric vein and hemorrhagic infarction of the bowel wall, an area also within the territory of the superior mesenteric vein. Liver cirrhosis, an enlarged spleen, and esophageal varices without rupture were also observed, but ulcers and variceal bleeding were not. Other organs showed no significant findings. His blood alcohol level was 0.14% w/v. Thus, this man died from severe hematochezia associated with SMVT due to liver cirrhosis and alcohol dehydration, which can lead to coagulopathy and rapid progress of thrombus formation. This is the first report on an alternate cause for massive gastrointestinal hemorrhage with a cirrhotic patient in a forensic autopsy.     

After the Fukushima Disaster: Japan's Nuclear Policy Change from 2011 to 2012

This article analyzes why and how the cabinet of Democratic Party of Japan (DPJ) decided in September 2012 to phase‐out nuclear power plants by 2039—the decision representing a large policy change from previous energy‐supply policy, in which the dependence on nuclear power would continuously grow. In doing so, this article examines the causal relationship between three factors identified on the basis of theoretical models explaining policy change; change in the governing coalition from the Liberal Democratic Party (LDP) to DPJ in 2009, the Fukushima accident, and the change in public opinion. Based on opinion poll and deliberative polling data, as well as discourse analyses of DPJ leaders’ statements, the article concludes that the change in public opinion on nuclear energy after the Fukushima accident was crucial in inducing the DPJ's decision to phase‐out nuclear power plants by affecting DPJ leaders' interests in vote‐ and office‐seeking. Finally, I discuss the applicability of the theoretical models developed on the basis of U.S. pluralistic cases to Japan and the issues to be explored for the further development of policy process models.     

An autopsy case of intraoperative death due to pulmonary fat embolism—possibly caused by release of tourniquet after multiple muscle-release and tenotomy of the bilateral lower limbs

We report a case of a juvenile male with muscle rigidity caused by cerebral palsy who experienced intraoperative sudden death due to pulmonary fat embolism after multiple muscle-release and tenotomy of the bilateral lower limbs. Data were obtained through review of the surgical and anesthesia records, as well as from autopsy and histopathological examination. All surgical procedures were performed within the same operation, beginning with the right lower limb and then proceeding with the left lower limb, with application of a pneumatic tourniquet to avoid intraoperative hemorrhage. Slight changes in the hemodynamics were noticed after release of the right tourniquet. Further, sudden onset of hypotension, severe bradycardia, and a marked decrease in percutaneously monitored oxygen saturation occurred just after release of the left tourniquet when the left limb was raised for casting. The patient died despite immediate and vigorous cardiopulmonary resuscitation. At autopsy performed 20 h after death, examination of the lungs revealed a pale surface, slight edema, and obvious fat droplets in the vessels at the cut surfaces. Histopathological examination with fat staining was notable for the presence of pulmonary fat embolism. These results suggest that restoration of venous return after removal of the tourniquet combined with massive fat embolism from dead spaces was the likely cause of death.     

Effects of a Dog-Assisted Intervention Assessed by Salivary Cortisol Concentrations in Inmates of a Japanese Prison

Physiological effects of a dog-assisted, stress management and communication training program conducted in a prison were investigated by assessing salivary cortisol concentrations of prison inmates before and after the sessions. The program was conducted with groups of inmates with psychiatric and/or developmental disorders. In the program, male inmates interacted with trained pet dogs and their volunteer handlers. Inmates evaluated their mood states before and after each session by using a questionnaire. Moreover, handlers evaluated the quality of the interaction with inmates after each session. Results indicated that the inmates’ cortisol values in most cases decreased following their participation in the sessions. The inmates who demonstrated stress reduction by decreasing cortisol values were diagnosed only psychiatric disorders, were aware of their mood improvement, and were evaluated by the handlers as having interacted well during the sessions. This indicated the validity of psychiatrists’ diagnoses, inmates’ self-evaluation, and handlers’ evaluation of inmates. Also, inmates who experienced a medium mood without mood changes had decreased cortisol values. It is suggested that these results are useful for predicting and selecting inmates who are expected to obtain effects by participating in the program. Moreover, it is suggested that providing feedback to inmates about changes in their salivary cortisol levels could help them better understand their psychophysical state, which could result in more effective stress management.