Sequenced allelic ladders and population genetics of a new STR multiplex system

The advent of PCR technology and use of short tandem repeat (STR) loci improves throughput and reduces costs whilst a high level of discrimination can be achieved. A new system, comprising seven STRs, was developed to compliment the existing systems. This paper describes the preparation of allelic ladders of the most commonly observed alleles of a new STR multiplex system (third generation; TGM multiplex); all alleles have been sequenced. Meioses studies estimated a mutation rate of 0-0.4% across loci. Statistical independence was investigated by employing exact tests; chi(2)-tests and excess homozygosity tests. The results demonstrated that the allele proportions do not differ from those expected and that there was no consequential dependence between loci. The discriminating power of the system was examined using 295 Caucasian, 140 Afro-Caribbean and 212 Asian unrelated samples, and was found to be approximately 1 in 50 million, 1 in 85 million and 1 in 20 million for each of these groups, respectively.     

A survey of extraction techniques for drugs of abuse in urine

Sixty nine participants in the United Kingdom national external quality assessment scheme for drugs of abuse in urine reported details of their sample extraction technique by questionnaire. Laboratories were categorised by differences in technique and their analytical test results compared for samples containing D-amfetamine 0.4 (4) and 0.8 (3) mg/l, morphine 0.4 (4) and 0.8 (4)mg/l, and benzoylecgonine 0.15/0.2 (2) and 0.45/0.5 (4) mg/l. Values in parentheses are numbers of samples. For amfetamine, there was no significant difference in the frequency of true positive results between liquid-liquid or solid phase extraction and the Toxi-Lab A system at 0.8 mg/l. Toxi-Lab A gave significantly fewer positives when operating below its specified threshold at 0.4 mg/l. Paradoxically, laboratories using >5 ml urine volume performed less well. Acidification of the extract before volume reduction gave significantly more true positives. For extraction of morphine, solid phase systems significantly outperformed both liquid-liquid and the Toxi-Lab A system at both 0.8 and 0.4 mg/l. No significant differences between extraction techniques were demonstrated for analysis of benzoylecgonine.     

Publicization,privatization, synthesis,tradition: options for public-private configuration (1)

In contrast to the attention paid to the privatization movement, relatively little heed has been given to a related and similarly large and important trend toward publicization. In publicization, a public sector organization proactively undertakes actions or responsibilities, and takes on characteristics, usually identified in that given setting with the private sector. An example of this movement appears at the local level of government: the ongoing funding available from Urban Development Action Grant and Community Development Block Grant programs is invested in development projects that private sector firms undertake. After discussing these examples, the article develops the concept of publicization and examines it in relation to the well-established concept of privatization. Based especially on the work of E.S. Savas, a typology is developed to place both publicization and privatization in a broader framework of public-private configurations. The typology indicates the relationship among publicization, privatization, and the additional categories of synthesis and tradition.     

“DAGGERING” AND THE REGULATION OF QUESTIONABLE BROADCAST MEDIA CONTENT IN JAMAICA

In 2008, a new style in Jamaican dancehall music and dance culture known as “Daggering” emerged. Daggering music and dancing, which included lyrics that graphically referred to sexual activities and a dance which has been described as “dry sex” on the dance floor, took Jamaica by storm. Unlike other dancehall traditions, however, Daggering went so main stream that both television and radio stations were airing audio and video recorded versions of the songs. The Broadcasting Commission of Jamaica was forced, because of the public controversy that evolved, to crack down on broadcasting and cable stations preventing them from playing any Daggering content. This article focuses on the subsequent clash between the government and the dancehall, and seeks to identify an appropriate method for monitoring and enforcing these new standards.     

Embedding the 'new economy' in Europe: a study in the institutional specificities of knowledge-based growth

Aspirations for a 'new economy' currently feature prominently in the economic policy debate within the EU. So pronounced is elite interest in the 'new economy' that the issue of knowledge-based growth dominated the Special European Council organized for Lisbon in May 2000. However, the Presidential Conclusions to that Council failed to address the question of whether the European economy is institutionally compatible with knowledge-based growth. The 'new economy' is currently most developed within the United States, and the institutional specificities of the American high-tech sector suggest that it may be impossible simply to import the 'new economy' into Europe. The EU may lack both the labour market and the capital market conditions necessary for successfully embedding the 'new economy' in Europe.