Additional predictions for forensic DNA phenotyping of externally visible characteristics using the ForenSeq and Imagen kits

Multiplex DNA typing methods using massively parallel sequencing can be used to predict externally visible characteristics (EVCs) in forensic DNA phenotyping through the analysis of single-nucleotide polymorphisms. The focus of EVC determination has focused on hair color, eye color, and skin tone as well as visible biogeographical ancestry features. In this study, we researched off-label applications beyond what is currently marketed by the manufacturer of the Verogen ForenSeq kit primer set B and Imagen primer set E SNP loci. We investigated additional EVC predictions by examining published genome wide sequencing studies and reported allele-specific gene expression and predictive values. We have identified 15 SNPs included in the ForenSeq kit panel and Imagen kits that have additional EVC prediction capabilities beyond what is published in the Verogen manuals. The additional EVCs that can be predicted include hair graying, ephelides hyperpigmented spots, dermatoheliosis, facial pigmented spots, standing height, pattern balding, helix-rolling ear morphology, hair shape, hair thickness, facial morphology, eyebrow thickness, sarcoidosis, obesity, vitiligo, and tanning propensity. The loci can be used to augment and refine phenotype predictions with software such as MetaHuman for missing persons, cold case, and historic case investigations.     

The European Union and Upheavals in its Neighborhood: A Force for Stability?

This article explores the EU’s action in the context of revolutionary upheavals in its neighborhood, focusing on two cases where the EU’s overall influence is relatively strong: Moldova in 2009 and Tunisia in 2010–2011. It analyzes the intertwining of the normative and strategic agendas by examining the goals, instruments and impact of EU action in each case. The findings highlight a limited and reactive role of the EU, a strong emphasis on stability and a shift towards a more normative engagement in response to external changes that made it easier to merge security- and value-oriented goals.     

Rethinking Dignity

The concept of dignity is widely debated as to its efficacy as a ground upon which to base respect particularly in relation to human rights. Traditional concepts of inherent dignity associate dignity with the possession of rationality and autonomy, which consequently excludes non-rational humans from being viewed as possessing inherent dignity and therefore equal and inherent worth. This paper offers a theory of inherent dignity based on an account of a common humanity within which all humans might be seen as possessing inherent worth and, therefore, deserving of being recognized and respected equally as ends in themselves. The theory is based on the communal practice and expectation of moral accountability, a practice that can be viewed as taking place universally both between and within human communities.     

Health advocacy on the margins: human rights as a tool for HIV prevention among LGBTI communities in Botswana

ABSTRACT

Human rights discourse in health advocacy is largely correlated with experiences of vulnerability, marginalisation and discrimination, with the global story of HIV activism the most visible example. In a domestic context where culture, consensus and belonging are highly valued, both human rights and lesbian, gay, bisexual, transgender and intersex (LGBTI) people face critiques of being foreign, un-African, new, individualistic and threatening to tradition. Why, when and how do civil society actors draw on human rights to advocate for LGBTI health in relation to HIV in Botswana? I examine this paradox through a case study of the key civil society actor in this sector. I argue that while formal structures and belief shape why the group engages with human rights, when and how human rights are invoked is shaped by perceptions of threat, cultural context, and belonging.     

On the origins of a fermier général: Family strategies over seven generations (late sixteenth through the late eighteenth centuries)

Family archives from the French ancien régime period offer the possibility of analyzing complex family strategy in the long term. The present article deals with the Brac family over seven generations, from the sixteenth to the eighteenth century, as it rose in prominence from a start in leatherworking to eventual high office in regional and Parisian public life. Over the centuries, the family accumulated land (the patrimony); used a combination of marital, geographical, professional, and kinship networks to advance its various members; maintained a policy of economic parsimony in its expenditures; and through various procedures favored a single heir in each generation. Though the various lines of the family were geographically dispersed, their activities in the main continued to focus on the ancestral family lands and the local society of its origins.     

An Integrated Theoretical Model of Sibling Violence and Abuse

Drawing on three theoretical perspectives (feminist, conflict, and social learning), an integrated analytical model of adolescence sibling violence and abuse is proposed. The model suggests that certain characteristics of the parents' relationship, various aspects of parent–child relations, and characteristics of the sibling relationship are major components in explaining sibling violence and abuse. These are mediated by individual sibling characteristics and sibling verbal conflict. The model should enable researchers to systematically examine, in a more holistic way, the factors related to violence and abuse, and to assess their relative importance in accounting for these phenomena.