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951.
Loss of heterozygosity detected in a short tandem repeat (STR) locus commonly used for human DNA identification 总被引:3,自引:0,他引:3
Rubocki RJ Duffy KJ Shepard KL McCue BJ Shepherd SJ Wisecarver JL 《Journal of forensic sciences》2000,45(5):1087-1089
Short tandem repeat (STR) markers are commonly used in basic genetic research and in human identification testing. Clinically, STRs can be used to study genetic alterations in tumors. A genetic deletion common to many types of cancer is referred to as the loss of heterozygosity (LOH). Numerous examples of LOH in cancer have been described and some have been mapped to areas located in close proximity to markers employed in human identity testing. Despite this fact, LOH has rarely been observed for STR loci commonly employed in forensic testing. Recently, for medico-legal purposes, we were asked to determine whether a tissue biopsy originated from a particular individual. For a reference source we assessed two specimens, one from normal tissue and one from cancerous tissue. When both reference specimens were used to generate DNA profiles, we observed LOH at one STR locus, D13S317. As demonstrated in other cancers only the cancerous biopsy demonstrated LOH. The forensic community should be cognizant of these unusual circumstances because, as identification of human DNA continues to be used more extensively, certain instances will arise in which reference material will not be readily available. In these situations, archived specimens may be employed as a reference source. Clinical specimens such as tissue biopsies should be used with caution if they have not been confirmed to contain normal tissue. 相似文献
952.
953.
Marc B Baudry F Douceron H Ghaith A Wepierre JL Garnier M 《Journal of forensic sciences》2000,45(1):216-222
Three cases of suicide by electrocution with low-voltage current were observed in five years (1994-1998) by medical clinical forensic examiners of an Emergency Forensic Unit of the Paris suburb among 2,000 external death examinations. The cases involved one woman, aged 72 and two men, aged 38 and 41. In the last two cases, electric burns were retrieved under bared electric wires, placed on the arms or fingers in order to realize a hand-to-hand electric circuit involving the heart muscle. In the other case, the electric circuit between mouth and foot also involved the heart muscle. Household low-voltage current delivered (220 V in France) had a sufficient strength to induce local muscular paralysis and heart fibrillation. In the three cases, blood samples taken have retrieved very high levels of muscular enzymes (CPK, LDH) correlated to the mechanism of electric death. The rareness of suicide by electrocution and its forensic characteristics are detailed in order to help the clinical forensic examiners, prosecutors, and police officers concerned by such death examinations. 相似文献
954.
955.
Sidorov VL Maiatskaia MV Liubimov IuA Babakhanian RV 《Sudebno-meditsinskaia ekspertiza》2000,43(4):29-30
Russian and foreign methods used in forensic medicine for detection of the semen in stains on material evidences are compared. The potentialities of quantitative immunofluorescence test for detection of the semen in stains on material evidences, developed at Bureau for Forensic Medical Expert Evaluations of the Leningrad region, are described. Unlike other methods used in Russia, this method detects the semen in stains in the absence of spermatozoa and in stains with very low amount of the semen. Our modification allows objective recording of the results with computer processing. The method is cheaper than its foreign analogs and its sensitivity is similar to them. 相似文献
956.
Dwyer LA 《Rutgers law review》2000,52(3):881-910
957.
958.
Toluene is recommended as an agent for extraction of nitrobenzene from biological material. Optimal conditions for nitrobenzene isolation from cadaveric human liver are defined and the results of isolation are quantitatively evaluated. 相似文献
959.
Determining the cause of death when a restrained person suddenly dies is a problem for death investigators. Twenty-one cases of death during prone restraint are reported as examples of the common elements and range of variation in these apparently asphyxial events. A reasonable diagnosis of restraint asphyxia can usually be made after ruling out other causes and collecting supportive participant and witness statements in a timely fashion. Common elements in this syndrome include prone restraint with pressure on the upper torso; handcuffing, leg restraint, or hogtying; acute psychosis and agitation, often stimulant drug induced; physical exertion and struggle; and obesity. Establishing a temporal association between the restraint and the sudden loss of consciousness/death is critical to making a correct determination of cause of death. 相似文献
960.
Mallak CT Milch KS Horn DF 《The American journal of forensic medicine and pathology》2000,21(1):79-82
The diagnosis of sudden infant death syndrome (SIDS) has been an enigma to medical examiners and coroners for decades. The recent drastic decrease in the number of SIDS cases has been associated with infants sleeping supine instead of prone. The apparent relation between sleeping position and SIDS has led to the marketing of several positioning sleep aids. We report a case of the improper use of one such device which resulted in the death of an infant. 相似文献