Quantification of human mitochondrial DNA using synthesized DNA standards

Successful mitochondrial DNA (mtDNA) forensic analysis depends on sufficient quantity and quality of mtDNA. A real-time quantitative PCR assay was developed to assess such characteristics in a DNA sample, which utilizes a duplex, synthetic DNA to ensure optimal quality assurance and quality control. The assay's 105-base pair target sequence facilitates amplification of degraded DNA and is minimally homologous to nonhuman mtDNA. The primers and probe hybridize to a region that has relatively few sequence polymorphisms. The assay can also identify the presence of PCR inhibitors and thus indicate the need for sample repurification. The results show that the assay provides information down to 10 copies and provides a dynamic range spanning seven orders of magnitude. Additional experiments demonstrated that as few as 300 mtDNA copies resulted in successful hypervariable region amplification, information that permits sample conservation and optimized downstream PCR testing. The assay described is rapid, reliable, and robust.     

Human Rights and Root Causes

The human rights movement has traditionally focused on documenting abuses, rather than attempting to explain them. In recent years, however, the question of the ‘root causes’ of violations has emerged as a key issue in human rights work. The present article examines this new (or newly insistent) discourse of root causes. While valuable, it is shown to have significant limitations. It foreshortens the investigation of causes; it treats effects as though they were causes; and it identifies causes only to put them aside. With these points in mind, the article counterposes an alternative approach in which the orienting concept is not root causes, but ‘planned misery’.     

Interrater reliability of Risk Matrix 2000/s

Actuarial risk assessment instruments for sexual offenders are often used in high-stakes decision making and therefore should be subject to stringent reliability and validity testing. Furthermore, those involved in the risk assessment of sexual offenders should be aware of the factors that may affect the reliability of these instruments. The present study examined the interrater reliability of the Risk Matrix 2000/s between one field rater and one independent rater with a sample of more than 100 sexual offenders. The results indicated good interrater reliability of the tool, although reliability varies from item to item. A number of factors were identified that seem to reduce the reliability of scoring. The present findings are strengthened by examining interrater reliability of the tool in the usual practitioner context and by calculating a range of reliability statistics. Strategies are suggested to increase reliability in the use of actuarial tools in routine practice.     

Planning for the succession of digital assets

Estate planners’ current strategies for the disposition of digital assets are quickly becoming out-dated. This is largely the result of ambiguities concerning proprietary rights of digital assets after death. When advising clients on how to plan for the succession of digital assets, it is important for estate planners to understand the nature of digital assets today and to recognize how these assets may evolve. In failing to take into account the evolving nature of digital assets, estate planners are liable to craft short-sighted and thus ineffective succession plans. As the popularity of digital executor businesses increases, estate planners ought to caution clients on their shortcomings. Additionally, legislatures must create statutes which outline the rights of individuals vis-à-vis online service providers.     

EU update

This is the latest edition of Baker &; McKenzie’s column on developments in EU law relating to IP, IT and telecommunications. This article summarises recent developments that are considered important for practitioners, students and academics in a wide range of information technology, e-commerce, telecommunications and intellectual property areas. It cannot be exhaustive but intends to address the important points. This is a hard copy reference guide, but links to outside web sites are included where possible. No responsibility is assumed for the accuracy of information contained in these links.     

Physical Abuse in a College Setting: A Study of Perceptions and Participation in Abusive Dating Relationships

This study explored the relationships between college students’ self-identification as recipients and/or perpetrators of abuse in their dating relationships and abusive behaviors reported. A cross-sectional examination of 1,530 undergraduate students was conducted. Results indicated that one in four college students were involved in a physically abusive dating relationship (involving at least two acts of physically violent behavior), and that over 85% of them failed to self-identify as ever having received and/or perpetrated any act of physical abuse. College students involved in abusive dating relationships were more likely than those not in abusive dating relationships to be in a relationship of a longer duration, to have been in a previous abusive dating relationship, and to be more accepting, in general, of physical abuse as a means of conflict resolution.     

Intergenerational Associations Between Trauma and Dissociation

The purpose of this study was to investigate intergenerational relationships between trauma and dissociation. Short and long term consequences of betrayal trauma (i.e., trauma perpetrated by someone with whom the victim is very close) on dissociation were examined in a sample of 67 mother–child dyads using group comparison and regression strategies. Experiences of high betrayal trauma were found to be related to higher levels of dissociation in both children and mothers. Furthermore, mothers who experienced high betrayal trauma in childhood and were subsequently interpersonally revictimized in adulthood were shown to have higher levels of dissociation than non-revictimized mothers. Maternal revictimization status was associated with child interpersonal trauma history. These results suggest that dissociation from a history of childhood betrayal trauma may involve a persistent unawareness of future threats to both self and children.     

Spatially offset Raman spectroscopy (SORS) for the analysis and detection of packaged pharmaceuticals and concealed drugs

Spatially offset Raman spectroscopy (SORS) is a powerful new technique for the non-invasive detection and identification of concealed substances and drugs. Here, we demonstrate the SORS technique in several scenarios that are relevant to customs screening, postal screening, drug detection and forensics applications. The examples include analysis of a multi-layered postal package to identify a concealed substance; identification of an antibiotic capsule inside its plastic blister pack; analysis of an envelope containing a powder; and identification of a drug dissolved in a clear solvent, contained in a non-transparent plastic bottle. As well as providing practical examples of SORS, the results highlight several considerations regarding the use of SORS in the field, including the advantages of different analysis geometries and the ability to tailor instrument parameters and optics to suit different types of packages and samples. We also discuss the features and benefits of SORS in relation to existing Raman techniques, including confocal microscopy, wide area illumination and the conventional backscattered Raman spectroscopy. The results will contribute to the recognition of SORS as a promising method for the rapid, chemically specific analysis and detection of drugs and pharmaceuticals.     

The Aftermath of the Macondo Discovery Well Spill: The Interplay of Claims to the Gulf Coast Claims Facility and Insurance Claims

The processing of claims for compensation arising from the April 20, 2010, explosion of the Deepwater Horizon offshore oil rig and its environmental and economic aftermath has proceeded simultaneously before the Gulf Coast Claims Facility and insurers. The criteria for recovery between these two recipients of claims differ significantly. Compensation from the facility has included claims for purely economic injury, whereas compensation from insurance generally requires, inter alia, a closer geographic or causal nexus to property damage. The resolution of compensation issues both by the facility and insurers will impact whether further disputes arise in the courts. Based upon similar experiences from September 11 and Hurricane Katrina, these issues are likely to continue for several years before they are fully resolved.