Genetic data for the 13 CODIS STR loci in Singapore Indians

Allele frequencies for the 13 CODIS short tandem repeat (STR) loci included in the AmpFISTR Profiler Plus and AmpFISTR Cofiler kits (Applied Biosystems, Foster City, USA) were determined in a sample of 174 unrelated Indians in Singapore.     

Quantification of propane in biological materials by head-space GC

Propane was measured in specimens taken from two persons who died from a LPG explosion in an apartment using head-space-GC/FID. Because of the variation of instrument performance and sample injection, the internal standard pentane was used. Calibration standards were prepared by injecting each calculated volume of pure propane gas into capped vials containing 2 ml of blood and 5 microl of internal standard. The calibration curve revealed good linearity from 0.09 microg/ml to at least 90 microg/ml. The method validation data also included repeatability and recovery. The propane quantities in blood, fat and brain tissue were between 0.27 and 71 microg/ml (microg/g) with the highest concentration observed in fat. The confirmation of propane was conducted by the means of solid phase micro-extraction and mass spectrometry.     

Representative Bureaucracy: Rethinking Substantive Effects and Active Representation

This article addresses perceived deficiencies in the study of representative bureaucracy by explaining and classifying the sources of passive representation's substantive effects. This classification is used to clarify existing empirical research and normative thinking on active representation. Doing so produces a more modest but more accurate interpretation of existing research findings and helps to indicate future research needs. It also reduces normative disagreement to a single source of substantive effects, namely bureaucratic partiality. Minority bureaucratic partiality is of dubious value for helping minorities, and bureaucratic partiality should generally be rejected. However, a passively representative bureaucracy increases in importance because of its other sources of substantive effects. This demonstrates the need to go beyond the passive–active distinction: It is more adequate and accurate to speak of representative bureaucracy and the sources of its substantive effects.     

A tide that does not lift all boats: the surge of remittances in post-disaster recovery in Tacloban City,Philippines

For the last forty years, remittances have been a lifeline for some Filipinos in times of crisis as well as everyday life. So, it was no surprise when the media and nongovernmental organizations credited the Philippine diaspora with playing a significant role in post-disaster recovery after Typhoon Haiyan/Yolanda struck in 2013. While remittances poured in after this disaster, they were differently experienced, had different impacts, and led to uneven post-disaster outcomes. This article highlights the class-based inequalities that structure access to and mobilization of remittances after post-disaster recovery. We compare the experiences of middle- and lower-class households in three communities heavily affected by Haiyan in Tacloban City, Philippines, to investigate who benefitted and who was excluded from remittances in this post-disaster recovery. The findings of the case study shed light on wider issues of poverty, class, and vulnerability in the Philippines.     

Contactless Visualization of Latent Fingerprints on Nonporous Curved Surfaces of Circular Cross Section

Nondestructive techniques for gathering evidence are important in the field of forensics. Due to the geometry of the substrates, nondestructive visualization of fingermarks on curved surfaces remains challenging. A novel contactless technique was developed for visualizing and recording fingermark patterns on nonporous curved surfaces of circular cross section. The technique utilizes a plane mirror to transmit rays from a light source to illuminate the area of interest for fingermark visualization. The fingermark acquisition system consists of a digital single‐lens reflex (SLR) camera, a plane mirror, and a white light source. Mathematical equations are used to calculate the mirror size. Experiments were performed on various curved surfaces to determine the feasibility and effectiveness of the technique. Spectral Image Validation and Verification (SIVV) was used to analyze the captured images. The results of this study indicate that the technique described here is able to reveal fingermark patterns on curved surfaces of circular cross section.     

Microbiota Composition and Pulmonary Surfactant Protein Expression as Markers of Death by Drowning

Pathological diagnosis of drowning remains a challenge for forensic science, because of a lack of pathognomonic findings. We analyzed microbiota and surfactant protein in the lungs for a novel diagnosis of drowning. All rats were divided into drowning, postmortem submersion, and control groups. The water, lungs, closed organs (kidney and liver), and cardiac blood in rats were assayed by targeting 16S ribosomal RNA of Miseq sequencing. Lung samples were analyzed by immunohistochemical staining for surfactant protein A. The closed organs and cardiac blood of drowned group have a lot of aquatic microbes, which have not been detected in postmortem submersion group. Furthermore, intra‐alveolar granular staining of surfactant protein A (SP‐A) was severely observed in the drowned group than the postmortem submersion and control groups. The findings suggested that the presence of aquatic microbiota in the closed organs and increased expression of SP‐A could be markers for a diagnosis of drowning.     

The limits of central bank independence for inflation performance

Public Choice - The independence of the central bank is routinely regarded as sacrosanct, at least for governments wishing to maintain credible monetary policy to meet inflation objectives. Yet...     

Analysis of ketamine and norketamine in urine by automatic solid-phase extraction (SPE) and positive ion chemical ionization-gas chromatography-mass spectrometry (PCI-GC-MS)

Ketamine (KT) is widely abused for hallucination and also misused as a "date-rape" drug in recent years. An analytical method using positive ion chemical ionization-gas chromatography-mass spectrometry (PCI-GC-MS) with an automatic solid-phase extraction (SPE) apparatus was studied for the determination of KT and its major metabolite, norketamine (NK), in urine. Six ketamine suspected urine samples were provided by the police. For the research of KT metabolism, KT was administered to SD rats by i.p. at a single dose of 5, 10 and 20mg/kg, respectively, and urine samples were collected 24, 48 and 72 h after administration. For the detection of KT and NK, urine samples were extracted on an automatic SPE apparatus (RapidTrace, Zymark) with mixed mode type cartridge, Drug-Clean (200 mg, Alltech). The identification of KT and NK was by PCI-GC-MS. m/z238 (M+1), 220 for KT, m/z 224 (M+1), 207 for NK and m/z307 (M+1) for Cocaine-D(3) as internal standard were extracted from the full-scan mass spectrum and the underlined ions were used for quantitation. Extracted calibration curves were linear from 50 to 1000 ng/mL for KT and NK with correlation coefficients exceeding 0.99. The limit of detection (LOD) was 25 ng/mL for KT and NK. The limit of quantitation (LOQ) was 50 ng/mL for KT and NK. The recoveries of KT and NK at three different concentrations (86, 430 and 860 ng/mL) were 53.1 to 79.7% and 45.7 to 83.0%, respectively. The intra- and inter-day run precisions (CV) for KT and NK were less than 15.0%, and the accuracies (bias) for KT and NK were also less than 15% at the three different concentration levels (86, 430 and 860 ng/mL). The analytical method was also applied to real six KT suspected urine specimens and KT administered rat urines, and the concentrations of KT and NK were determined. Dehydronorketamine (DHNK) was also confirmed in these urine samples, however the concentration of DHNK was not calculated. SPE is simple, and needs less organic solvent than liquid-liquid extraction (LLE), and PCI-GC-MS can offer both qualitative and quantitative information for urinalysis of KT in forensic analysis.     

Neo-liberalism in post-crisis South Korea: Social conditions and outcomes

South Korea's post-war development pattern has been considered to be a developmental state model, where the basic institutional framework was the “state-banks-chaebol nexus.” Since the financial crisis of 1997, however, the country has been swiftly transformed from the developmental state model and has acquired more neo-liberal characteristics. This has been made possible through intense reforms implemented by the post-crisis South Korean government under the IMF's guidance and encouraged by domestic political, ideational, and social conditions. Among those, significant conditions for this swift transformation include chaebols' legitimacy problems, neo-liberal consensus among the domestic elite, democracy hijacked by neo-liberalism, and the role of some NGOs. As social outcomes of the transformation, we find the enlarged presence of transnational capital, increased labour flexibility, inequality and poverty, and an increasing cultural gap in the country. Huge social costs of such neo-liberal transformation necessitate an alternative path of political economy.     

A Simple Method of VNTR D1S80 Locus Allelic Ladder Construction for Capillary Electrophoresis‐based Genotyping

VNTR D1S80 locus genotyping has been largely replaced in forensics by STR. As the statute of limitations on murder cases was abolished in the Republic of Korea in July 2015, the demand for re‐analysis of DNA from unresolved murder cases has increased. The National Forensic Service includes several recorded D1S80 genotypes as crucial clues. Here, we re‐established the D1S80 analysis system using capillary electrophoresis and confirmed the reproducibility of the system by comparison with the genotypes of eight DNA samples that had been analyzed using PAGE in 2006. In addition, we created an allelic ladder via new methodology using flanking region sequences. A single DNA sample (K562) and seven primers were used for the new ladder, which contains 12 alleles. Although artificial owing to the use of the flanking region rather than repeat unit reduction, the method is rapid and simple, and could be applicable in any laboratory.