Analysis of active ricin and castor bean proteins in a ricin preparation, castor bean extract, and surface swabs from a public health investigation

In late February 2008, law enforcement officials in Las Vegas, Nevada, discovered in a hotel room, a copy of The Anarchist Cookbook, suspected castor beans and a "white powder" thought to be a preparation of ricin. Ricin is a deadly toxin from the seed of the castor bean plant (Ricinus communis). The United States regulates the possession, use, and transfer of ricin and it is the only substance considered a warfare agent in both the Chemical and the Biological Weapons Conventions. Six samples obtained from the hotel room were analyzed by laboratories at the Centers for Disease Control and Prevention using a panel of biological and mass spectrometric assays. The biological assays (real time-PCR, time resolved fluorescence and cytotoxicity) provided presumptive evidence of active ricin in each of the samples. This initial screen was followed by an in-depth analysis using a novel, state-of-the-art mass spectrometry-based ricin functional assay and high sensitivity tandem mass spectrometry for protein identification. Mass spectrometric analysis positively identified ricin and confirmed that in each of the samples it was enzymatically active. The tandem mass spectrometry analysis used here is the most selective method available to detect ricin toxin. In each sample, ricin was unequivocally identified along with other R. communis plant proteins, including the highly homologous protein RCA120. Although database searches using tandem mass spectra acquired from the samples indicated that additional controlled substances were not present in these samples, the mass spectrometric results did provide extensive detail about the sample contents. To the best of our knowledge following a review of the available literature, this report describes the most detailed analysis of a white powder for a public health or forensic investigation involving ricin.     

CHALLENGES OF DEVELOPMENT IN BURUNDI： A CASE STUDY OF PROTECTION OF REFUGEES AND IDPS IN POST-CONFLICT RECONCILIATION AND TRANSITIONAL JUSTICE

Violations of human rights and genocide in Burundi are among the most contentious issues which continue to attract the attention of academic discourse. This paper is concerned with the question of human rights violations in Burundi from a historical and comparative perspective. It seeks to trace the root causes of Burundi＇s sullied human rights record over 52 years since independence from Belgium in 1962, the role of the military in human rights violations, including mass killings of civilians and extra-judicial executions of political opponents and the fact that the post- conflict constitutional architecture has not succeeded in establishing accountability and responsibility for these violations; in providing truth, justice and reparations to the victims and in putting an end to the culture of impunity which seems to be entrenched in Burundian society. Moreover, by analyzing critically the results from interviewing 113 Burundians and 16 non-Burundians, this paper argues that there will be no political stability enduring peace without addressing these issues in a comprehensive manner.     

Predictive accuracy of dynamic risk factors for aboriginal and non-aboriginal sex offenders: an exploratory comparison using STABLE-2007

Although Aboriginal offenders are overrepresented in Canadian prisons, there is limited research examining the extent to which commonly used risk factors and risk scales are applicable to Aboriginals. Aboriginal (n = 88) and non-Aboriginal (n = 509) sex offenders on community supervision were compared on the dynamic risk factors of STABLE-2007. Data on sexual, violent, any crime, and any recidivism (including breaches) were collected with an average follow-up of 3.4 years. Aboriginal offenders scored significantly higher than non-Aboriginal offenders on STABLE-2007 total scores and on several items measuring general criminality. STABLE-2007 did not significantly predict recidivism with Aboriginal offenders (although it did for non-Aboriginals). The general antisociality items were generally significantly less predictive for Aboriginals than non-Aboriginals, whereas items assessing sexual self-regulation and relationship stability predicted similarly for both groups. These exploratory results suggest that Aboriginal sex offenders are a higher-needs group but that some STABLE-2007 items are not predictive with this population.     

Institutionalism Old and New*

Abstract
The author deals with the legal theoretical approach that has been labelled "legal institutionalism." An old and a new version of this approach are singled out: The old one is identified with the theory defended by the Italian public lawyer Santi Romano in the first half of this century; the second one is seen in the recent work by Ota Weinberger and Neil MacCormick. After a short presentation of Romano's work, his ideas and the development proposed by MacCormick and Weinberger are compared. Similarities and differences between the two versions of institutional theory are worked out. A coda hints at an ambiguity in the definition of institution proposed by the "new" institutionalism.     

Theoretical issues and social indicators: A societal process approach

Well-being involves various levels: the individual, the institutional-distributive, and the societal. These levels are interrelated. Social indicators of well-being must be theoretically based so as to take into account these levels. A societal process model is proposed to describe the levels of society and the nature of well-being at each level. Social indicator types regarding the output and distribution of well-being, the effect of policy manipulatable and nonmanipulatable inputs, and the secondary consequences of inputs are suggested and examples are provided.The ideas presented herein have evolved from the authors' participation in a research project conducted for the U.S. Agency for International Development (AID/csd—3642), but do not necessarily reflect the views of that agency. Thanks are extended to J. Steven Picou, James Copp, Jon Alston, Bill Howard, and Ben Crouch, as well as to the then editor of this journal, Garry Brewer, and several anonymous reviewers who were kind enough to provide both cogent critique and encouragement. Journal Paper No. J-8808 of the Iowa Agriculture and Home Economics Experiment station, Ames, Iowa. Project No. 1837.     

Stray bullets and “mushrooms”: Random shootings of bystanders in four cities, 1977–1988

Bystanders killed by bullets not specifically intended for them have long been a very small part of the homicide problem. But the frequency of press accounts of such killings and woundings has apparently increased nationally in recent years. To test this impression, we compiled all shootings of bystanders hit at random and reported in the published indexes of theNew York Times, theLos Angeles Times, and theWashington Post for 1977–1988, as well as a key word computer search of stories in theBoston Globe. We found a rapid increase in both bystander woundings and killings since 1985 in all four cities. The base rate was quite low, and total bystander deaths appear to comprise less than 1% of all homicides in these cities. Nonetheless, the numbers were large enough to show that most bystanders reported shot in New York and Los Angeles are victims of random shootings into crowds, rather than single stray bullets striking a lone individual mushroom. The reverse was true in Boston and Washington, with the effect of much lower rates of bystanders reported shot in those cities.