Uses of the NIST 26plex STR assay for human identity testing

Ongoing work at the U.S. National Institute of Standards and Technology has focused on the characterization of 26 autosomal STR loci for human identity testing. These 26 loci are in addition to the existing 13 U.S. core loci and those found in PowerPlex16 and Identifiler commercial STR typing kits. The amplification of the 26 loci has been optimized for degraded extracts in unique miniplex panels and also for reference samples as a single reaction 26plex assay. A study has been performed comparing genotypes obtained with the 26plex primers to those with miniplex panels for allele drop out and concordance. The forensic utility of the 26plex assay was evaluated for situations where additional loci are beneficial. The utility of this large multiplex was also tested in a case involving DNA extracted from degraded bone samples. The 26plex can serve as a low-cost assay (compared to commercially available kits) useful for both sorting comingled remains and providing additional markers for increased statistical support for samples that require “non-trio” family references for human identification.     

Ethnic Networks

Active research on a wide range of political contexts centers on ethnicity's role in collective action. Many theories posit that information flows more easily in ethnically homogeneous areas, facilitating collective action, because social networks among coethnics are denser. Although this characterization is ubiquitous, little empirical work assesses it. Through a novel field experiment in a matched pair of villages in rural Uganda, this article directly examines word‐of‐mouth information spread and its relationship to ethnic diversity and networks. As expected, information spread more widely in the homogeneous village. However, unexpectedly, the more diverse village's network is significantly denser. Using unusually detailed network data, we offer an explanation for why network density may hamper information dissemination in heterogeneous areas, showing why even slight hesitation to share information with people from other groups can have large aggregate effects.     

Understanding motives for aggression in forensic psychiatric patients: a preliminary study

The primary aim of this study was to explore motivations underpinning aggression among men detained within conditions of high security. Thirty men residing at a high secure psychiatric hospital completed self-report measures, including the Aggression Motivation Questionnaire, Revised EXPAGG and Barratt Impulsiveness Scale-IIr. The Historical items of the Historical, Clinical and Risk-Management (HCR-20) and the Psychopathy Checklist-Screening Version were rated. A subsample of participants agreed to complete a functional assessment on an aggressive incident that had occurred during their placement (n = 9). Increased psychopathy and impulsivity, and the presence of historical risk items were predicted to associate with higher levels of both aggression motivation and beliefs supportive of aggression. Young age at first violent incident and personality disorder related positively to aggression motivation. Thematic analysis conducted on the functional assessments identified social recognition, emotion regulation, communication and protection as functions underpinning aggression. Results are discussed with regards to their implication for violence treatment and assessment, with a focus on motivation recommended.     

The innovative personality? Policy making and experimentation in an authoritarian bureaucracy

Why do local officials in an authoritarian bureaucracy experiment with policy, even when directed not to do so by central‐level officials? This study suggests that policy experimentation in this institutional environment can best be understood as an interaction between the structure in which local officials are embedded and individual‐level personality attributes. Leveraging a new data set from a series of original surveys with local policy makers in mainland China, conducted between 2016 and 2018, we discern three baseline personality types: authoritarian, consultative, and entrepreneurial. We thereafter examine the individual‐level characteristics of local officials who will innovate irrespective of a centralization of bureaucratic power and interests, as currently experienced under Chinese President Xi Jinping. We find that local policy makers engage in policy innovation when they are more focused on resolving governance problems and that increased risk reduces but does not eliminate their willingness to innovate. Based on these findings, we contend that future studies of policy innovation should use an evolutionary framework to examine the interaction between preferences and selection pressures.     

EVIDENTIARY ISSUES WITH PARENTAL ALIENATION SYNDROME

Expert testimony on unsubstantiated social science syndromes such as the Parental Alienation Syndrome (PAS) has been increasingly admitted in courtrooms across the United States. This is a problem because a trier of fact is making a determination based on theories that are inaccurate or incorrect. To remedy this, the standards of admissibility for expert testimony must be heightened. The broad discretion given to trial judges in determining admissibility should be reevaluated and a new rule of evidence for social science testimony should be adopted.     

Expert Opinion, Agency Characteristics, and Agency Preferences

David E. Lewis Woodrow Wilson School of Public and International Affairs, Princeton University, Princeton, NJ 08540 e-mail: delewis{at}princeton.edu e-mail: clinton{at}princeton.edu (corresponding author) The study of bureaucracies and their relationship to politicalactors is central to understanding the policy process in theUnited States. Studying this aspect of American politics isdifficult because theories of agency behavior, effectiveness,and control often require measures of administrative agencies'policy preferences, and appropriate measures are hard to findfor a broad spectrum of agencies. We propose a method for measuringagency preferences based upon an expert survey of agency preferencesfor 82 executive agencies in existence between 1988 and 2005.We use a multirater item response model to provide a principledstructure for combining subjective ratings based on scholarlyand journalistic expertise with objective data on agency characteristics.We compare the resulting agency preference estimates and standarderrors to existing alternative measures, discussing both theadvantages and limitations of the method. Authors' note: We thank Tom Hammond, George Krause, and JoshuaTucker for helpful comments. We are grateful to Simon Jackmanand Shawn Treier for generously providing their code and oursurvey respondents for their time and expertise.     

杆状病毒表达的猪传染性胃肠炎病毒钉状糖蛋白在其血清学诊断上的应用

利用从猪传染性胃肠炎病毒（ＴＧＥＶ）Ｍｉｌｅｒ株（Ｍ５Ｃ）克隆建立的一株重组杆状病毒（Ｒ２－２）表达的重组ＴＧＥＶ钉状糖蛋白（Ｓ），建立了一种阻断酶联免疫吸附试验（ＥＬＩＳＡ）来检测猪群抗ＴＧＥＶ抗体。试验表明，用重组病毒Ｒ２－２接种ｓｆ９细胞（ｓｐｏｄｏｐｔｅｒａｆｒｕｇｉｐｅｒｄａ）所表达的重组蛋白量在接种后第７２ｈ达到最高值；该重组蛋白能与抗ＴＧＥＶＳ蛋白Ａ位点的单克隆抗体特异性结合。用阻断ＥＬＩＳＡ检测６８份来自无ＴＧＥＶ感染或ＴＧＥＶ（Ｐｕｒｄｕｅｌ５）免疫的仔猪或母猪血清，结果与ＴＧＥＶ蚀斑减数试验完全相符，灵敏度和特异性均为１００％。同时检测来自ＴＧＥＶ试验免疫猪、美国部分地区猪群、我国进口猪以及我国国内猪群血清６２７份，阳性检出率分别为８２．６１％、６１．６２％、５８．３３％和２９．１０％；用中和试验（微量中和试验或病毒蚀斑减数试验）检测的ＴＧＥ血清抗体阳性率分别为８０．８７％、５９．６０％、５６．１１％和３０．６０％，两种方法对抗ＴＧＥＶ抗体的检出率没有显著差异。     

STR Profiles from DNA Samples with "Undetected" or Low Quantifiler™ Results

Abstract:  Screening methods capable of identifying DNA samples that will not yield short tandem repeat (STR) profiles are desired. In the past, quantitation methods have not been sensitive enough for this purpose. In this study, low level DNA samples were used to assess whether Quantifiler™ has a minimum quantitation value below which STR profiles would consistently fail to be detected. Buccal swabs were obtained and the DNA extracted, quantified, and serially diluted to concentrations ranging from 0.002 to 0.250 ng/μL. Samples were analyzed once with Quantifiler™, followed by Profiler Plus™ amplification and capillary electrophoresis analysis. An absolute minimum value below which STR results were unobtainable could not be defined. From the 96 low level samples tested, STR loci (including one full profile) were successfully amplified and detected from 27% of the samples "undetected" by Quantifiler™. However, no STR alleles were detected in 73% of these "undetected" samples, indicating that Quantifiler™ data may be useful for predicting STR typing success.     

Looking for ‘New Ideas That Work’: county innovation in China’s health system reforms—the case of the New Cooperative Medical Scheme

Sub-national governments in China have substantial responsibility for policy development as well as for direct implementation of circumscribed policy options set out by higher levels of government, and much policy discourse emphasizes the importance of sub-national flexibility and creativity in policy implementation. Discourses of government innovation aim to encourage local initiative in policy formulation and solving of systemically-important policy problems, and policy experimentation/innovation are increasingly credited as important elements of the Chinese government toolbox in managing reform. Recent studies have tended to treat experimentation/innovation as systemic phenomena, and there are few analyses of how local governments respond to central ‘experimental’ policy frameworks and develop locally- or systemically-useful policy solutions. Given concerns around the capacity of local governments, this is highly relevant in understanding how locally-generated policy relates to systemic reform. The article presents a case study of a low tech and ‘second best’ reimbursement mechanism developed sub-nationally under the New Cooperative Medical Scheme, China’s rural health insurance framework, and its spread and incorporation into national policy. It argues for the importance of local government development of ‘appropriate’ policy mechanisms (jizhi) as underpinning central reforms and system adaptation.