Recovery of human DNA profiles from poached deer remains part 2: Improved recovery protocol without the need for LCN analysis

Although poaching is a common wildlife crime, the high and prohibitive cost of specialised animal testing means that many cases are left un-investigated. We previously described a novel approach to wildlife crime investigation that looked at the identification of human DNA on poached animal remains (Tobe, Govan and Welch, 2011). Human DNA was successfully isolated and amplified from simulated poaching incidents, however a low template protocol was required which made this method unsuitable for use in many laboratories. We now report on an optimised recovery and amplification protocol which removes the need for low template analysis.Samples from 10 deer (40 samples total — one from each leg) analysed in the original study were re-analysed in the current study with an additional 11 deer samples. Four samples analysed using Chelex did not show any results and a new method was devised whereby the available DNA was concentrated. By combining the DNA extracts from all tapings of the same deer remains followed by concentration, the recovered quantity of human DNA was found to be 29.5 pg ± 43.2 pg, 31 × greater than the previous study. The use of the Investigator Decaplex SE (QIAGEN) STR kit provided better results in the form of more complete profiles than did the AmpF?STR® SGM Plus® kit at 30 cycles (Applied Biosystems). Re-analysis of the samples from the initial study using the new, optimised protocol resulted in an average increase of 18% of recovered alleles. Over 17 samples, 71% of the samples analysed using the optimised protocol showed sufficient amplification for comparison to a reference profile and gave match probabilities ranging from 7.7690 × 10^? 05 to 2.2706 × 10^? 14.The removal of low template analysis means this optimised method provides evidence of high probative value and is suitable for immediate use in forensic laboratories. All methods and techniques used are standard and are compatible with current SOPs. As no high cost non-human DNA analysis is required the overall process is no more expensive than the investigation of other volume crime samples. The technique is suitable for immediate use in poaching incidents.     

Stirred,Shaken, or Blended

Abstract

Research documents the variation in levels of offending and the official response to boys' and girls' misbehavior (see Chesney-Lind & Sheldon 2004 for a nice summary of this work). Our research questions develop from this literature. We first expect differences in girls and boys at intake to a residential program for low-to-medium risk offenders, and then we also expect differences in official responses to girls and boys during their aftercare experience. Our findings confirm the literature—girls and boys differ in seriousness of committing offenses. Additionally, girls and boys are treated differently during their time in juvenile justice programming. For example, girls are monitored more closely than boys and receive less serious penalties for similar levels of rule violations. We find no difference between boys and girls in their odds of receiving a serious sanction. While traditional theoretical attempts to explain girls' misbehavior are often described with the expression “add women and stir,” our findings suggest that both behavior of and official response to boys' and girls' activities show that girls are not simply stirred into the male mix but are responded to differently than boys and in ways that illustrate conventional gender socialization.     

University Leaders and the Public Agenda: Talking About Women and Diversity in STEM Fields

We undertake an investigation of public statements and pronouncements from leaders at various universities to gain insight into institutional values and environments relative to women and their participation and advancement in science, technology, engineering, and mathematics (STEM) and other disciplinary fields. Employing content analysis techniques to focus on terminological use and keyword interdependencies in these speeches, our findings indicate that, while gender equality is addressed as a separate topic in its own right, university leaders raise issues of gender in the context of STEM participation primarily in conjunction only with other topics. As expected, the data also support arguments suggesting diversity in general as an important goal espoused in the rhetoric of university representatives. Questions remain, however, concerning whether these speeches presage concrete institutional commitments and responses relative to the achievement of diversity, gender equality, and gender equity in the STEM professoriate.     

Extremity in Congress: Communications versus Votes

I propose a theory of legislator‐to‐constituent communication that describes a relationship between the types of votes a legislator reveals and the partisan composition of her constituency. To test this theory, I use an original data set of 40,000 official communications containing 30,000 vote revelations from the 111th Congress. I find evidence substantiating this theory; the extent to which a legislator endeavors to appear more ideologically extreme in communications varies systematically with the relative amounts of different types of voters in her district. This result is contrasted with an analysis of voting extremism where I find that the ideological preferences of donors better explain voting patterns.     

Changing How the System Responds to Status Offenders: Connecticut's Families with Service Needs Initiative

Concerned by the number of status offenders in secure detention and the processing of noncriminal behavior through the juvenile and family courts, Connecticut began efforts to reform their approach to troubled and troublesome youth in 2005 (Weithorn, 2005). Between 2005 and 2007, Connecticut enacted a series of legislative changes to both improve services for troubled youth and Families with Service Needs (FWSN), and achieve systematic reforms for processing status offenses. At a systems level, the reforms sought to divert non‐criminal behaviors from formal court processing and prevent secure confinement for status offenders who violated court orders related to their behavior. Connecticut's attempt at second‐order, or system change, is the focus of this outcome evaluation.     

Communications: Exchange on the Philippines

Abstract

In the December 1973 issue you printed an article by Ms. Luzviminda Francisco in which she attempted to sketch in the “nature of America's policy of aggression, the depth of popular mass resistance to the American forces and the duration of the struggle….” (p. 3) in order to move against “Filipino false consciousness” (p. 2) of the American connection in the history of the Philippines. It is a brief survey and rather well done, although one may quibble about the relationship of excesses (what a tame word compared to the record she presents!) of war and the nature of imperialism as well as implying that there was a “nation,” a “Philippine society,” or a “national struggle” (p. 3) before the late 19th century, at the earliest.     

Feeling guilty to remain innocent: the moderating effect of sex on guilt responses to rule-violating behavior in adolescent legal socialization

Legal socialization researchers have ignored the role of emotions such as guilt to explain rule-violating behavior (RVB). The purpose of Study 1 was to determine if anticipated guilt or guilt proneness was a better predictor of RVB. Participants were 325 university students who completed an online questionnaire. Correlations indicated that both measures were related significantly to RVB; however, when both were entered into a multiple regression as predictors, only anticipated guilt was significant. This suggested that anticipated guilt was a stronger predictor of RVB than guilt proneness. The purpose of Study 2 was to investigate the effects of anticipated guilt on future RVB while controlling for the integrated legal socialization variables. Participants were 283 middle school and 187 high school students. Multiple regression analyses were conducted to predict students' future engagement in RVB. Anticipated guilt predicted RVB for middle school and high school students. However, sex moderated these effects. Male students low in anticipated guilt committed more RVBs than male students high in guilt. Female high school students showed a similar effect but not at the same magnitude as the male students. Guilt had no significant effect on RVB for female middle school students. Implications for the findings are discussed.