Combination of DNA-based and conventional methods to detect human leukocyte antigen polymorphism and its use for paternity testing

In cases of disputed paternity, the scientific goal is to promote either the exclusion of a falsely accused man or the affiliation of the alleged father. Until now, in addition to anthropologic characteristics, the determination of genetic markers included human leukocyte antigen gene variants; erythrocyte antigens and serum proteins were used for that reason. Recombinant DNA techniques provided a new set of highly variable genetic markers based on DNA nucleotide sequence polymorphism. From the practical standpoint, the application of these techniques to paternity testing provides greater versatility than do conventional genetic marker systems. The use of methods to detect the polymorphism of human leukocyte antigen loci significantly increases the chance of validation of ambiguous results in paternity testing. The outcome of 2384 paternity cases investigated by serologic and/or DNA-based human leukocyte antigen typing was statistically analyzed. Different cases solved by DNA typing are presented involving cases with one or two accused men, exclusions and nonexclusions, and tests of the paternity of a deceased man. The results provide evidence for the advantage of the combined application of various techniques in forensic diagnostics and emphasizes the outstanding possibilities of DNA-based assays. Representative examples demonstrate the strength of combined techniques in paternity testing.     

Czech Republic

European Journal of Political Research -     

Optimal effort allocation by U.S. Senators: the role of constituency size

Legislators in a representative democracy are modeled as being able to allocate a fixed amount of effort between two objectives: national policymaking and local benefit-seeking. The model predicts that the effort allocated to local benefit-seeking should be a negative function of the population size of a legislator's constituency. We empirically test and confirm this prediction by examining the manner in which United States senators allocate their personal staff between home state and Washington D.C. offices.     

Proliferation of neopatrimonial domination in Turkey

ABSTRACT

Literature on Turkey’s post-2011 authoritarian turn – especially after the eruption of the 2013 nationwide Gezi Protests – adopts modern concepts such as ‘dictatorship’, ‘authoritarianism’, ‘totalitarianism’, ‘one-party government’, ‘party-state fusion’, and even ‘fascism’ mainly in order to pin down the nature of the Justice and Development Party (AKP, Turkish acronym) or depict the current character of Turkey’s regime. Through engaging the pre-modern concept of neopatrimonialism, which is derived from Max Weber’s concept of patrimonialism, this paper argues that Turkey’s encounter with authoritarianism is deeply associated with the proliferation of neopatrimonial domination, into which the legacy of patronage politics, fracture of security power, and the metastasis of crony capitalism have been conflated. This article argues that neopatrimonial features have always, to a degree, marked state-society relations in Turkey. Furthermore, this article suggests neopatrimonial characteristics started to dominate Turkey’s modern legal structure under the AKP, which led to a state crisis culminating in the 2016 attempted coup. However, despite the fact that neopatrimonialism cannot be argued as a pathological deviation from modern-legal domination, this paper concludes that tension exists between the crony capitalism-based economic model of neopatrimonalism and Turkey’s decades-long market-based capitalism.     

Gelatinase A in human dentin as a new biochemical marker for age estimation

Dentin proteins from 24-individual permanent molars from patients aged 15 to 73 years were sequentially extracted, with guanidinium chloride (G1-extract) and then with EDTA; after demineralization, the material was again extracted with guanidinium chloride (G2-extract). Extracts were analyzed by SDS-PAGE electrophoresis and the gels were processed for zymography to detect gelatinolytic activities. The patterns of gelatinase A distribution differed in the different dentin protein fractions, and the changes varied with age. Significant differences were detected in gelatinase A in G2-extracts between individual younger than 20 years old and the rest of the sample (chi2exp = 19.429; 1 d.f.; p < or = 0.001). The incidence of true and false positives and negatives, and sensitivity and specificity for the presence of gelatinase A in dentin extracts, were calculated. Determination of gelatinase A in human dentin may be a useful marker to estimate age, especially when other morphological methods are of limited usefulness.