Characterization of 26 miniSTR loci for improved analysis of degraded DNA samples

An additional 20 novel mini-short tandem repeat (miniSTR) loci have been developed and characterized beyond the six previously developed by our laboratory for a total of 26 non-CODIS miniSTR markers. These new markers produce short PCR products in the target range of 50-150 base pairs (bp) by moving the primer sequences as close as possible-often directly next to the identified repeat region. These candidate loci were initially screened based on their small amplicon sizes and locations on chromosomes currently unoccupied by the 13 CODIS STR loci or at least 50 Mb away from them on the same chromosome. They were sequenced and evaluated across more than 600 samples, and their population statistics were determined. The heterozygosities of the new loci were compared with those of the 13 CODIS loci and all were found to be comparable. Only five of the new loci had lower values than the CODIS loci; however, all of these were much smaller in size. This data suggests that these 26 miniSTR loci will serve as useful complements to the CODIS loci to aid in the forensic analysis of degraded DNA, as well as missing persons work and parentage testing with limited next-of-kin reference samples.     

Pig-mentation: postmortem iris color change in the eyes of Sus scrofa

Experienced forensic pathologists and examiners may be familiar with the phenomenon of postmortem iris color change; however, only Knight, Simpson's forensic medicine, Arnold, London, 1997; Ref. 1 and Saukko and Knight, Knight's forensic pathology, 3rd ed., Arnold, London, 2004; Ref. 2 have referred to it in the literature, and to date, there have been no published scientific research studies on this taphonomic artifact. A controlled experiment was conducted of postmortem changes to isolated Sus scrofa eyes. The eyes (n = 137) were separated into three groups and each sample was observed for 3-day postmortem at a different temperature. In addition, a Sus scrofa head was obtained to observe postmortem changes of eyes in situ. All isolated blue eyes in the experiment, at room temperature and higher, changed to brown/black within 48 h. The in situ blue eye, at room temperature, turned brown/black within 72 h. If iris color consistently changes postmortem in humans, then this taphonomic artifact must be incorporated into victim identification protocol, including disaster victim identification software, and autopsy reports to prevent inaccurate victim identification and inappropriate exclusion from the identification process.     

缅甸佛教与王权

本文考察了缅甸上座部佛教发展的历史及其特征,指出缅甸佛教的久远流传与王权的护持密不可分。王权、僧伽与佛法之间共存共荣是缅甸政治发展、僧伽繁荣与佛教流传的根本原因。佛教作为缅甸民族文化的核心与根基,对缅甸社会产生了广泛而深远的影响。     

Sustainable development at the sharp end

This paper takes an actor-oriented approach to understanding the significance for policy and practice of field-worker experience at the interface between project and people. It is set in the context of an Indian project which aims to reduce poverty through sustainable, participatory agricultural change, based on low-cost inputs, catalysed by village-based project staff. Diaries kept by such staff are analysed to reveal how the social position of field-workers enables and constrains their interactions within and without the project, and the ways in which ‘street level bureaucrats’ shape projects through their discretionary actions. They show the Village Motivators struggling to communicate project objectives, to establish their roles and distinguish themselves fromother village-level bureaucrats, to negotiate participation, to overcome hostility to Participatory Rural Appraisal, to arbitrate access to consultants and seniors, to interpret project objectives and lobby for changes in these without admission of failure, and finally to develop a shared vocabulary of participation and belief in success. Some of the implications for participatory approaches are that there may be significant contradictions between sustainability and participatory development.     

Toward the Assessment of Procedural and Distributive Justice in Resolving Family Disputes

The first phase of this study focused on the development of comprehensive, conceptually integrated measures of procedural and distributive justice in the context of family decision making. In the second phase, these measures were used to examine older adolescents' justice appraisals of specific family disputes and the relation of these justice appraisals to family systems functioning along dimensions of conflict and cohesion. A Family Justice Inventory was constructed, which included two global indices (one for procedural justice and one for outcome fairness) and 13 subscales: 9 measuring specific facets of the procedural justice construct and 4 measuring specific dimensions of the distributive justice construct. Factor analysis revealed that the 13 Family Justice Inventory subscales could be reduced to 5 interpretable procedural justice factors (personal respect, status recognition, process control, correction, and trust) and 4 interpretable distributive justice factors (decision control, need, equality, and equity). Using procedural justice factor scores in regression analyses, personal respect, status recognition, correction, and trust each accounted for unique variance in family conflict and family cohesion. Using distributive justice factor scores in regression analyses, both decision control and need accounted for unique variance in family conflict and family cohesion. Using both procedural and distributive justice factor scores in regression analyses, personal respect, status recognition, and trust each accounted for unique variance in both family conflict and family cohesion. Additionally, equity also accounted for unique variance in family conflict but not family cohesion and the direction of the relationship was positive, that is, more equity in resolving specific family disputes was associated higher levels of general family conflict.