Bone as a biomarker of acute fluoride toxicity

The use of bone as a biomarker for chronic and acute exposure to fluoride salts has been suggested, but there are no data published about its use to evaluate lethal intoxication. One hundred and sixty rats were divided into eight groups that received a single oral intubation dose from 0 (control) to 90 mg F/kg as NaF. The animals' time of death was recorded and their femurs were removed for fluoride analysis. Acid-soluble fluoride was determined in the whole bone and on the surface (periosteal), using an ion specific electrode. The data showed a statistically significant relationship between fluoride dose and the number of deaths (P<0.0001). A statistically significant relationship was also found between fluoride dose and fluoride concentration ([F]) in either the whole femur (P<0.0017), on the surface (P<0.0001) or for the ratio periosteal [F]/whole [F] (P<0.0001). However, the [F] on the femur surface was more closely correlated with mortality than that in the whole bone, showing statistically significant differences among the lethal doses and control (P<0.05). The data suggest that the ratio [F] periosteal bone/[F] whole bone, is a biomarker for acute fluoride toxicity.     

Results of the GEP-ISFG collaborative study on two Y-STRs tetraplexes: GEPY I (DYS461, GATA C4, DYS437 and DYS438) and GEPY II (DYS460, GATA A10, GATA H4 and DYS439)

A collaborative exercise was carried out by the Spanish and Portuguese ISFG Working Group (GEP-ISFG) in order to evaluate the performance of two Y-chromosome STR PCR tetraplexes, which include the loci DYS461, GATA C4, DYS437 and DYS438 (GEPY I), and DYS460, GATA A10, GATA H4 and DYS439 (GEPY II). The participating laboratories were asked to type three samples for the eight markers, using a specific amplification protocol. In addition, two control samples, with known haplotypes, were provided. The results obtained by the 13 different participating laboratories were identical, except for two laboratories that failed to type correctly the same two samples for GATA C4. By sequence analyses, two different GATA C4 allele structures were found. One control sample (allele 21) and two questioned samples (allele 22, correctly typed by all the laboratories, and allele 25) presented the following repeat structure: (TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)n, but different from the one found for allele 26 in one sample included in this exercise, as well as in the second control sample (allele 23), namely (TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)2(TGTA)2(TCTA)n. The collaborative exercise results proved that both Y-tetraplexes produce good amplification results, with the advantage of being efficiently typed using different separation and detection methodologies. However, since GATA C4 repeat presents a complex structure, with alleles differing in sequence structure, efficient denaturing conditions should be followed in order to avoid typing errors due to sizing problems.     

Identification of two fire victims by comparative nuclear DNA typing of skeletal remains and stored umbilical tissues

We describe here our collaborative efforts in identifying 2 fatalities of a fire disaster by using a variety of identification techniques. Postmortem findings in both cases were reinforced using Short Tandem Repeat (STR) DNA technology to establish with a high degree of certainty the identities of 2 child victims. STR markers used in the present study include HUMAMEL, HUMCSFIPO, HUMTHO1, HUMvWA, HUMFES/FPS, HUMF13A01, HUMFOLP23, D8S3O6, HUMFGA, and HUMTPOX. Unambiguous identification was made possible through matching DNA profiles generated from skeletal remains with those from umbilical tissues. These tissues were kept by their mothers in accordance with a Philippine tradition and were submitted for DNA analysis. Of the DNA profiles generated from exhumed bone samples of 21 child victims, comparison with the genetic profiles of children A and B obtained from umbilical tissues showed consistent DNA matches with remains 1756 and 1758, respectively.     

A method for exposing the intraosseous portion of the carotid arteries and its application to forensic case work

A method that allows extraction of the intraosseous portion of the carotid arteries is presented, along with four cases to illustrate its application to forensic case work. The method described permits a cosmetically acceptable reconstruction.     

Does alcohol make a difference? Within-participants comparison of incidents of partner violence

Episodes of husband-to-wife violence in which the husband consumed alcohol were compared with episodes of husband-to-wife violence, reported by the same individuals, in which the husband was not drinking. Among this community sample of newlyweds, wife reports, but not husband reports, indicated that violent episodes in which the husband was drinking included more acts of violence and were more likely to involve severe violence. Both wife and husband reports indicated that wives were more likely to be physically aggressive in husband drinking episodes compared to sober episodes. However, whereas wives reported that their aggressive behavior was a response to husband aggression, husbands reported that wives were more likely to initiate violence in these episodes. Violent episodes that include alcohol may be more severe and more mutually violent than sober episodes. Discrepancies between husband and wife reports may reflect differences in recall or self-serving biases.     

Shot range estimation based on pellet distribution in shots with a pump-action shotgun

In the present study, shots from different ranges were fired using a non-choked pump-action shotgun (Hugsan) of calibre 12 with a bore length of 52cm and pellets of various sizes. The aim of the study is range estimation from the distribution of pellets.Using pellets of 2, 3.5, 5 and 8mm size, 5 shots were fired from: 0.7, 0.8, 0.9, 1, 2, 3, 5, 10, 15, 20 and 25ms. The maximum distance between the pellets in vertical and horizontal direction was measured. Statistical evaluation of the data revealed a highly significant correlation for each pellet type; the constant values a and b were calculated to be used in the linear regression equation y=a+bx.As a conclusion, the linear regression equation as well as table data may allow range determination within acceptable ranges of error for the same barrel length and diameter for the same cartridge type. Under identical conditions they may also provide information for pellets of comparable size.     

Instructions for Authors

Instructions for Authors

Instructions for Authors     

The external occipital protuberance: can it be used as a criterion in the determination of sex?

Sex determination of a corpse can be problematic in cases where the body is damaged. Useful criteria would assist in the identification of sex in such cases. The goal of this study is to determine the usefulness of the external occipital protuberance (EOP) in the determination of sex, especially in lateral cranium radiographs. The types and configurations of the EOP were investigated on normal lateral cranium radiographs of 1000 subjects (500 males and 500 females) and 694 dry-skull remains (371 males and 323 females) from a 16th Century Anatolian population for the purpose of sex determination. In the radiographic examination, the incidence of less prominent (Type 1) EOP is found to be 85.4% in females whereas 17.8% in males. The spine type (Type 3) EOP is found to be 63.4% in males and to be 4.2% in females. On the other hand, studies of dry-skull remains revealed the incidence of Type 1 EOP to be 67.5% in females and Type 3 EOP to be 55.2% in males. The crest type (Type 2) EOP is approximately equal in both sexes and is found to be less valuable for sex determination in both groups.