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41.
Pujol ML Cirimele V Tritsch PJ Villain M Kintz P 《Forensic science international》2007,170(2-3):189-192
This work presents the validation of a new immunological assay, the One-Step enzyme-linked immunosorbent assay (ELISA) tests from International Diagnostic Systems Corp. for the screening of drugs of abuse (cannabis, amphetamines, opiates, and cocaine) in human hair, with subsequent GC-MS confirmation. After decontamination and segmentation into small pieces, 50 mg of hair sample were incubated in 1 ml of methanol during 16 h at 40 degrees C. A 100 microL aliquot was collected and evaporated to dryness in presence of 100 microL of methanol/hydrochloric acid (99:1, v/v) to avoid amphetamines loss. The dried extract was dissolved in 100 microL of the "sample and standard diluent" solution included in the kit. This solution was submitted to analysis according to the recommended instructions of the manufacturer. During the validation phase, GC-MS confirmations were conducted according to our fully validated and published methods for opiates, cocaine, cannabis, and amphetamines determinations in hair. In a last development step, these procedures were slightly modified to directly confirm ELISA results by GC-MS using the methanolic extract. Ninety-three specimens were simultaneously screened by the ELISA tests (103 for tetrahydrocannabinol (THC)) and confirmed by GC-MS. Twenty were found positive for cannabis (THC: 0.10-6.50 ng/mg), 21 for cocaine (0.50-55.20 ng/mg), 24 for opiates (6-acetylmorphine (6-AM): 0.20-11.60 ng/mg, MOR: 0.20-8.90 ng/mg, codeine (COD): 0.20-5.90 ng/mg), and 13 for amphetamines (AP: 0.20 and 0.27 ng/mg, methamphetamine (MAP): 0.30 and 1.10 ng/mg, methylenedioxymethamphetamine (MDMA): 0.22-17.80 ng/mg). No false negative results were observed according to the Society of Hair Testing's (SoHT) cutoffs (0.5 ng/mg for cocaine, 0.2 ng/mg for opiates and amphetamines, and 0.1 ng/mg for THC). The One-Step ELISA kits appear suitable due to their sensitivity and specificity for drug of abuse screening in hair. This technology should find interest in workplace drug testing or driving license regranting, especially when many samples have to be tested with a high rate of negative samples, as ELISA is an easy and high-throughput method. 相似文献
42.
Kintz P Evans J Villain M Salquebre G Cirimele V 《Forensic science international》2007,173(2-3):171-174
Diphenhydramine is one of the first effective antihistamine agents to have been discovered. The compound is also used for its sedative and antiemetic effects. The first case involving repetitive sedation linked to the use of diphenhydramine as a drug-facilitated crime and subsequent impairment of a 9-year-old female victim is reported. Due to the long delay between the alleged crime and clinical examination, collection of blood or urine was of little value. Hence, the laboratory developed an original approach based on hair testing by LC-MS/MS. A single strand of hair from the victim was sampled about 7 weeks after the last suspected administration and was cut into small segments. After cutting into small pieces, about 20 mg of hair per segment was incubated overnight in a phosphate buffer (pH 8.4). The aqueous phase was extracted with 5 ml of a mixture of methylene chloride/diethyl ether (80/20), in presence of diazepam-d5, used as internal standard (IS). The hair extract was separated on an XTerra MS C18 column using a gradient of acetonitrile and formate buffer. Detection was based on two daughter ions: transitions m/z 256.2-152.1 and 167.1 and m/z 289.9-154.0 for diphenhydramine and the IS, respectively. In the hair of the child, diphenhydramine was detected at concentrations in the range 33-39 pg/mg, depending on the segment. 相似文献
43.
The aim of this study was to evaluate the accuracy and reliability of the rapid analyte measurement platform (RAMP) for presumptive identification of Bacillus anthracis spores. Test samples consisted of serial dilutions of spore preparations of several Bacillus species, including B. anthracis, which were tested, using the RAMP Anthrax test cartridge, according to the manufacturer's instructions. The fluorescence labelled antibody-antigen complexes were detected in the portable reader after 15 min following sample addition. Dilutions of common environmental and household powders were also tested to identify possible false positive results. B. anthracis spores were identified reliably in test samples containing more than 6000 spores. The test kits were highly specific, showing no cross reactivity with other Bacillus species or any environmental powders tested. The RAMP system for detection of B. anthracis spores, from environmental samples, showed consistent results under a variety of analytical conditions, enabling the trained user to provide a rapid, accurate preliminary risk assessment of a suspected bioterrorism incident. 相似文献
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Fatalities due to 3,4-methylenedioxymethamphetamine (MDMA, "ecstasy") are rare in Austria, although the use of designer drugs has become quite common. This is the first published case of a fatal MDMA intoxication in Austria. A 19-year-old girl died after the consumption of ecstasy tablets in the apartment of a friend. Blood analysis gave a concentration of MDMA as 3.8 mg/L and traces of its metabolite MDA. Cannabinoids were found as well. This case shows that the consumption of MDMA, without physical stress, can lead to death. 相似文献
47.
Marion van San 《Trends in Organized Crime》2011,14(4):281-297
Women living at the edge of poverty often find themselves attracted to ‘dangerous’ men. Many of these women find themselves uncontrollably drawn to men who have made a career in crime. Curaçao women living with men involved in the drug economy in the Netherlands or on the island of Curaçao are no exception to this rule. The fact that these women are attracted to this kind of man often leads to an environment where boys are raised to become ‘dangerous’ sons. Once grown up, these boys will in turn attract female contemporaries who exist at the margins of society. This article attempts to explain how this vicious circle can be broken. 相似文献
48.
Hamid Algar Richard Tapper Ali Banuazizi Helena Cobban Joseph A Kechichian 《Third world quarterly》2013,34(2):1024-1058
Yet more on the Islamic revival The Islamic Impulse. Edited by Barbara Freyer Stowasser. London & Sydney: Croom Helm with Center for Contemporary Arab Studies, Georgetown University, Washington, DC. 1987. 329pp. £25.00hb From Nationalism to Revolutionary Islam. Edited by Said Amir Arjomand. London: Macmillan, with St Antony's College, Oxford. 1984. 256pp. £33.00hb Islam and the Political Economy of Meaning: Comparative Studies of Muslim Discourse. Edited by William R Roff. London: Croom Helm. 1987. 295pp. £25.00hb Minorities and the problem of the state The Elementary Structures of Political Life: Rural Development in Pahlavi Iran. Grace E Goodell. Oxford: Oxford University Press. 1985. 362pp. n/p Islam and Resistance in Afghanistan. Olivier Roy. Cambridge: Cambridge University Press. 1986. 253pp. £27.50hb/£9.95pb The State, Religion, and Ethnic Politics: Afghanistan, Iran and Pakistan. Edited by Ali Banuazizi and Myron Weiner. Syracuse, New York: Syracuse University Press. 1986. 464pp. $35.00hb Iran's revolution reappraised Iran: A Revolution in Turmoil. Edited by Haleh Afshar. London: Macmillan. 1985. 262pp. £25.00hb/£8.95pb The Iranian Revolution and the Islamic Republic. Edited by Nikki R Keddie and Eric Hooglund. Syracuse, New York: Syracuse University Press. 1986. 246pp. $14.95pb The Spirit of Allah: Khomeini and the Islamic Revolution. Amir Taheri. London: Hutchinson. 1985. 350pp. £14.95hb All Fall Down: America's Tragic Encounter with Iran. Gary Sick. New York: Random House. 1985. 366pp. $19.95hb Revolutionary Iran: Challenge and Response in the Middle East. R K Ramazani. London: Johns Hopkins University Press. 1986. 311pp. £21.60hb The Left in Contemporary Iran: Ideology, Organization and the Soviet Connection. Sepehr Zabih. London: Croom Helm. 1986. 239pp. £25.00hb Iran: At War with History. John W Limbert. Boulder, Colorado: Westview (distributed in the UK by IPI). 1987. 186pp. £24.00hb The discovery of the Lebanese Shia Militant Islamic Movements in Lebanon: Origins, Social Basis, and Ideology. Edited by Marius Deeb. Washington, DC: Center for Contemporary Arab Studies, Georgetown. University. 1986. 27pp. n/p The Vanished Imam: Musa al‐Sadr and the Shia of Lebanon. Fouad Ajami. London: I B Tauris. 1986. 228pp. £15.95hb Amal and the Shi'a: Struggle for the Soul of Lebanon. Augustus Richard Norton. Austin, Texas: University of Texas Press. 1987. 238pp. $10.95pb The beguiling Gulf Cooperation Council Majlis al‐Taawun al‐Khaliji: Al‐Itar al‐Siyasi wal‐Istratiji (The Gulf Cooperation Council: The Political and Strategic Framework). Abdallah Fahd al‐Nafisi. London: Ta‐Ha Publishers. 1982. 72pp. £2.50pb Al‐Itar Al‐Qanuni wal‐Siyasi Li‐Majlis al‐Taawun al‐Khaliji (The Legal and Political Framework of the Gulf Cooperation Council). Abdallah al‐Ishal. Riyadh, Saudi Arabia. 1983. 291pp. $10.00pb Majlis al‐Taawun al‐Khaliji: Azmat al‐Siyasat wal‐Shariyat (The Gulf Cooperation Council: The Crisis of Politics and Legitimacy). Majid al‐Majid. London: Ta‐Ha Publishers. 1986. 99pp. £2.50pb Majlis al‐Taawun li‐Dual al‐Khalij al‐Arabiyyah: Ruyat Mustaqbaliyat, Dirasat Qanuniyat, Siyasiyat, Iqtisadiyat (The Gulf Cooperation Council: Future Developments—A Legal, Political and Economic Study). Yahya Halmi Rajab. Kuwait: Maktabat al‐Arubat lil‐Nashr wal‐Tawzi. 1983. 528pp. $12.00pb 相似文献
49.
Fran Tonkiss Stephen Greasley Catherine McGlynn Marion Reiser 《Local Government Studies》2013,39(4):587-593
The New Politics of British Local Governance. Edited by Gerry Stoker. Macmillan, 2000. Pp.xvii + 294, £16.99 pb. Local Political Leadership. By Steve Leach and David Wilson. Policy Press, 2000. Pp.v + 228. £17.99. Structural Reform of British Local Government: Rhetoric and Reality. By Michael Chisholm. Manchester University Press, 2000. Pp.194. £40. The Careers of Councillors: Gender, Party and Politics. By Catherine Bochel and Hugh M. Bochel. Ashgate, 2000. Pp.143. £37.50 hb. Communicating Successfully in Groups: A Practical Guide for the Workplace. By Marie Reid and Richard Hammersley. Routledge, 2000. Pp.188. £10.99 pb. Using Psychology in Management Training: The Psychological Foundations of Management Skills. By David A. Statt. Routledge, 2000. £14.99. Housing Associations and Housing Policy: A Historical Perspective. By Peter Malpass. Macmillan Press, 2000. Pp.298. £16.99 pb. The State of UK Housing: A Factfile on Dwelling Conditions. By Philip Leather and Tanya Morrison. The Policy Press, 2000. £15.95 hb. Allocating Social Housing: Law and Practice in the Management of Social Housing. By Hal Pawson, David Mullins and Siobhan McGrath. Lemos and Crane, 2000. £18.95 pb. 相似文献
50.
Sparse literature exists on the topic of intimacy appeals in political advertising, so in this study Horton and Wohl's para-social theory was applied to explain the “relationship” in Israel between television performers (politicians) and spectators (voters) through the intimacy concept. Altogether, 442 political ads from two Israeli election campaigns (1996 and 1999) were analyzed to test three hypotheses on the use of intimacy appeals across campaigns and political parties. The intimacy appeal measure consisted of five intimacy techniques and was thus characterized as a macro-level variable. These techniques appear in the codebook as five questions that examine whether the spots present words of intimacy, intimate situations, families of politicians, nonverbal expressions, and children. The results indicate that intimacy appeals in political spots are quite popular. Religious and left-leaning parties tended to use intimacy appeals significantly more than nonreligious and right-leaning parties. We conclude that politicians indeed use the same intimacy strategies and techniques as other television performers do; therefore, para-social theory accords with the concept of intimacy in political advertising as much as in other communication settings. 相似文献