Characterization and validation studies of powerPlex 2.1, a nine-locus short tandem repeat (STR) multiplex system and penta D monoplex

In order to increase the power of discrimination for human identification purposes, a nine-locus short tandem repeat (STR) multiplex, the GenePrint PowerPlex 2.1 system (PowerPlex 2.1) developed by Promega Corporation and a separate pentanucleotide-repeat locus, Penta D, were tested. This megaplex system includes the highly polymorphic loci FGA, TPOX, D8S1179, vWA, Penta E, D18S51, D21S11, TH01, and D3S1358 and may be used in combination with the eight-locus STR multiplex, the GenePrint PowerPlex 1.1 system (PowerPlex 1.1) that has been previously developed. Three of the loci, TPOX, TH01 and vWA, have been included in both systems for quality control purposes. As with PowerPlex 1.1, PowerPlex 2.1 is also based on a two-color detection of fluorescent-labeled DNA products amplified by polymerase chain reaction (PCR) and provides a valuable tool for accurate and rapid allele determination. The primer sequences used in the PowerPlex 2.1/Penta D system are also presented in this report. To meet the "Quality Assurance Standards for Forensic DNA Testing Laboratories" (FBI), we tested the efficiency and reproducibility of the PowerPlex 2.1/PentaD system by several validation studies that were conducted as a joint project among seven laboratories. Validation tests included concordance studies, sensitivity, and species specificity determination, as well as performance in forensic and environmentally impacted samples. The results produced from these tests demonstrated the consistency and reliability of the PowerPlex 2.1/Penta D system.     

Comparing inmate perceptions of two residential substance abuse treatment programs

Drug and alcohol abuse by criminal offenders remains at an all time high. Residential substance abuse and treatment (RSAT) programs were developed to address the drug and alcohol treatment needs of inmates in prisons. Typically, such programs range in length from 6 to 12 months, have an Alcoholics Anonymous and/or Narcotics Anonymous component, and occur in a therapeutic community environment. Some programs also include a cognitive self-change component. Inmate participation in their programming is crucial to the success of a therapeutic community treatment environment, yet there is little research on the perceptions of RSAT inmates regarding their programming. In this research, the authors describe, compare, and contrast the perceptions of inmate clients of two RSAT programs in a rural mountain state.     

Information Technology Outsourcing by State Governments in Australia

Outsourcing of IT services is being undertaken at a rapid rate within the public sector in Australia. This paper investigates the adoption of this practice by a sample of state government enterprises and departments from South Australia, Victoria and New South Wales which have tendered long-term IT outsourcing contracts.
The motivation, decision-making process and extent of outsourcing are examined in further detail. In addition, the tendering process, contract and risk management are investigated to determine whether consideration has been given to the full economic costs involved in entering into longer term contracts.     

Leona helmsley: The construction of a woman tax evader

Conclusion To paraphrase marjorie Kornhauser’s famed observation, a taxcollection system for revenue only is a chimera. If, for the American woman, tax collection were only, and only ever, about revenue, then they would have constantly and consitently collect it from us. When we did not have what they say we should have, then they would penalise us. The fact of the matter is, that when they do collect it from us, it is, more often than not, because they have been successful in their efforts to help us sustain ourselves in an image which they have created. One problem with self-assessment is that exemplary penalties are allowed; indeed, arede rigeur. The latest example of a feminine image which spurrerd the American tax collector to action is the Leona Helmsley equation of consumption—plus—feminine equals “ off with her head”. If only for this reason, self assessment demands to be, as Handelman and Green might argue, politicised with the feminine voice. With thanks to Peter Alldridge.     

Factors related to cigarette smoking during adolescent pregnancy

Previous research suggests that maternal smoking during pregnancy remains prevalent, particularly among adolescents. However, little is known about the factors related to smoking during adolescent pregnancy. The goal of the present study is to identify intrapersonal, familial, and peer factors that are related to smoking during adolescent pregnancy, and to determine the relative degree to which they affect this behavior. Interviews were conducted with 241 unmarried pregnant adolescents who planned to carry their pregnancies to term. Consistent with previous studies, 27% of the respondents reported daily smoking during pregnancy, and whites reported higher rates of use than members of other racial groups. Smoking during adolescent pregnancy was related to intrapersonal, familial, and peer factors. The results of a regression analysis suggest that perceived parental disapproval of smoking during pregnancy, friends' cigarette use, and race play a particularly important role in this behavior. The implications of these findings for preventive programs are discussed.Research reported here and the preparation of this report were supported by Grant DA-05208 from the National Institute on Drug Abuse.Received M.A. in sociology from the University of Washington. Research interests include health behaviors, and the relationship between social stratification and health.Received Ph.D. in social welfare from the University of Washington. Research interests are in adolescent development, gender issues in adolescent development, and health promotion and problem prevention with children and adolescents. Received Ph.D. in sociology from the University of Washington. Research interests are adolescent problem behaviors, and particularly adolescent sexual decision making.Received M.S. in educational psychology from the University of Wisconsin—Madison. Research interests: adolescent substance use, pregnancy and parenthood, and peer relations.     

Adventures of Feminism: Simone de Beauvoir's Autobiographies,Women's Liberation,and Self-Fashioning

While The Second Sex is usually taken as Simone de Beauvoir's major theoretical contribution to feminism, in the 1960s and 1970s it was very often through her autobiographies – especially Memoirs of a Dutiful Daughter, The Prime of Life, and Force of Circumstance, along with novels such as She Came to Stay and The Mandarins – that her feminist ideas were most thoroughly absorbed. The autobiographies became nothing less than a guide for the fashioning of a new kind of feminine self. Where The Second Sex had intimated that a significant aspect of human liberation lay in women not losing their identity or their sense of self in those of men, it was the autobiographies which suggested and demonstrated in great detail how this might be done. In them, the rejection of conventional marriage and children was no mere slogan, but the foundation of what seemed to young female readers to be a fascinating and challenging life. In this paper, I reflect on de Beauvoir and her historical and contemporary relevance: first through reminiscence and re-reading of the autobiographies themselves; then with an historical examination of how they were read, taking Sydney, Australia, as my example; and finally by offering some reflections on subsequent feminist critique.     

The personality correlates of differential verbal and mathematical ability in talented adolescents

This paper describes the personality correlates of differential verbal and mathematical ability in very bright adolescent boys (N=182). The results indicate that there are few mean personality differences between two groups of boys formed on the basis of high scores on measures of either verbal or quantitative ability. On the other hand, within each of these groups there are dependable personality correlates of differences between verbal and quantitative ability. In particular, boys with high verbal relative to their quantitative scores seem independent, rational, reflective, and mature, regardless of their vocational or academic preferences.Work on this paper was supported by a grant from the Spencer Foundation, Chicago, Illinois.Received her Ph.D. in psychology from the Johns Hopkins University. Major interests are personality theory and medical psychology.Received his Ph.D. in psychology from the Johns Hopkins University. Major interests are education and developmental psychology.Received his Ph.D. in psychology from the University of California at Berkeley. Major interests are personality theory and moral development.