Review Essay

Abstract

So far in modern world history, social revolutions, though they have entailed elements of class conflict, have plainly not conformed to Marx's theoretical expectations or moral vision. They have occurred in agrarian countries caught behind foreign competitors, not in the most advanced capitalist industrial nations. And even those revolutions that have expropriated domestic capitalist classes in the name of socialist ideals have hardly resulted to date in the prosperous, democratic communist societies envisaged by Marx. (Skocpol, p. 292)     

Evaluation and quantification of nuclear DNA from human telogen hairs

Nuclear DNA was extracted from human telogen hairs from 60 individuals. Six to nine hairs from each individual were individually extracted. The amount of DNA recovered from each individual varied greatly, and most samples yielded a quantity of 550 pg or less per hair. A selective extraction buffer was used to remove epithelial cell DNA and the amount of exogenous DNA was determined. DNA was also quantified by real time PCR using three different sized amplicons targeting an Alu sequence. The results were used to determine the state of degradation of the extracted DNA. Different quantities of sample (<100 pg, 100-500 pg, >500 pg) were amplified with the Miniplex kits to determine the minimum DNA template required for successful amplification. DNA recovered from hair showed degradation; however, partial profiles were obtained for those samples containing at least 60 pg using MiniSTRs.