Comparison of quantitative PCR and culture-based methods for evaluating dispersal of Bacillus thuringiensis endospores at a bioterrorism hoax crime scene

Since the anthrax mail attacks of 2001, law enforcement agencies have processed thousands of suspicious mail incidents globally, many of which are hoax bioterrorism threats. Bio-insecticide preparations containing Bacillus thuringiensis (Bt) spores have been involved in several such threats in Australia, leading to the requirement for rapid and sensitive detection techniques for this organism, a close relative of Bacillus anthracis. Here we describe the development of a quantitative PCR (qPCR) method for the detection of Bt crystal toxin gene cry1, and evaluation of the method's effectiveness during a hoax bioterrorism event in 2009. When combined with moist wipe sampling, the cry1 qPCR was a rapid, reliable, and sensitive diagnostic tool for detecting and quantifying Bt contamination, and mapping endospore dispersal within a mail sorting facility. Results from the cry1 qPCR were validated by viable counts of the same samples on Bacillus-selective agar (PEMBA), which revealed a similar pattern of contamination. Extensive and persistent contamination of the facility was detected, both within the affected mailroom, and extending into office areas up to 30m distant from the source event, emphasising the need for improved containment procedures for suspicious mail items, both during and post-event. The cry1 qPCR enables detection of both viable and non-viable Bt spores and cells, which is important for historical crime scenes or scenes subjected to decontamination. This work provides a new rapid method to add to the forensics toolbox for crime scenes suspected to be contaminated with biological agents.     

Automated Mapping of Explosives Particles in Composition C‐4 Fingerprints*†‡

Abstract: A method is described to perform automated mapping of hexahydro‐1,3,5‐trinitro‐1,3,5‐triazine (RDX) particles in C‐4 fingerprints. The method employs polarized light microscopy and image analysis to map the entire fingerprint and the distribution of RDX particles. This method can be used to evaluate a large number of fingerprints to aid in the development of threat libraries that can be used to determine performance requirements of explosive trace detectors. A series of 50 C‐4 fingerprints were characterized, and results show that the number of particles varies significantly from print to print, and within a print. The particle size distributions can be used to estimate the mass of RDX in the fingerprint. These estimates were found to be within ±26% relative of the results obtained from dissolution gas chromatography/μ‐electron capture detection for four of six prints, which is quite encouraging for a particle counting approach. By evaluating the average mass and frequency of particles with respect to size for this series of fingerprints, we conclude that particles 10–20 μm in diameter could be targeted to improve detection of traces of C‐4 explosives.     

青少年文本和视觉资料中的全球化因素(续完)

第三类:"性别暗示性"(正文有全球化因素,背页没有,20个样本) 在14-17岁学生中,这一类占子样本的21%:比较而言,这一类中女生比例多于男生(25%比15%).一种说明是,虽然这些文本中有全球化因素,主要的话语实际上是一种性别化的.以下是这类文本中的三个个案.     

Identity in adolescence: Present and future self-concepts

This paper reports an investigation to test the hypothesis that anxiety concerning a concept of the self in the future will increase with age during adolescence, while anxiety relating to the self in the present will not. Subjects included a total of 80 adolescent boys from schools in a working class area of London, divided into three groups of 12-year-olds, 14-year-olds and 16-year-olds. The results strongly substantiated the hypothesis, and are discussed in relation to Erikson's theory of an adolescent identity crisis.At present Senior Lecturer in Psychology at The London Hospital Medical College (University of London). Is both a Developmental and Clinical Psychologist, with interests in adolescence and the preschool years.Medical student at The London Hospital Medical College, having a special interest in psychology.     

Hallucinations with zolpidem and fluoxetine in an impaired driver

A 54-year-old male was observed driving erratically. The subject displayed both horizontal and vertical nystagmus and poor balance on field sobriety tests (FST's). Further observations included slow movements, breathing, and speech, extremely poor coordination, lack of convergence, and pupils with slow reaction to light. The subject related he was seeing roadway lines doubled and felt a hallucinogenic effect. Breath alcohol results were negative. Blood tests found zolpidem and fluoxetine in addition to hydrocodone at therapeutic levels. This case is presented as an example of hallucinogenic effects from the combination of zolpidem and serotonin reuptake inhibitors.     

Conceiving harm: disability discrimination in assisted reproductive technologies

Applying the Americans with Disabilities Act (ADA) to denials of treatment by assisted reproductive technology (ART) practitioners raises particularly challenging legal and ethical issues. On the one hand, the danger that physicians will inappropriately deny treatment to patients with disabilities is especially worrisome in the context of ARTs, given the widespread stigma associated with reproduction by individuals with disabilities. On the other hand, patients' disabilities may sometimes have potentially devastating implications for any child resulting from treatment, including the possibility that the child will be born with life-threatening or seriously debilitating impairments. Some physicians have strong ethical objections to helping patients become pregnant in the face of such risks. In this Article, Professor Coleman develops a framework for applying the ADA to disability-based denials of ARTs that addresses these competing considerations. In recognizing risks to the future child as a potential defense to a disability discrimination claim, Professor Coleman rejects the view of some commentators that such risks are relevant to reproductive decisions only if the child is likely to suffer so much that he or she would prefer not to exist. Instead, he proposes that, when a patient's disabilities create significant risks to the future child, the question should not be whether the child's life is likely to be so awful that nonexistence would be preferable, but how the risks and benefits of the requested treatment compare to those associated with other available reproductive and parenting options. Professor Coleman provides a theoretical justification for adopting this comparative framework, and examines how ADA precedents developed in other contexts should be applied to decisions about ARTs.     

Obstacles to organizational change: the creation of the Canadian Space Agency

Abstract: This article begins with the observation that the recently established Canadian Space Agency possesses very few of the organizational attributes that its early promoters had envisioned. The agency is much less autonomous, for example, and is located much farther from the industry than was originally expected. We describe three rather distinct perspectives on organizational change and argue that each one has a contribution to make in understanding the evolution of the Canadian Space Agency away from its initial conception. As backdrop to this theoretical argument we outline the structure of the space industry, the various initiatives undertaken to guide industry development, and the circumstances surrounding the creation of the space agency. In the final section of the article, drawing on interviews with public officials and private actors, we show how, in creating the space agency, bureaucrats, politicians and industry representatives were unable to surmount several key obstacles previewed in the introductory section. Sommaire: Cet article commence par le constat que l'Agence spatiale canadienne qui vient d'être mise sur pied ne possède que très peu des attributs organisationnels que ses premiers promoteurs avaient envisagés. Par exemple, l'agence est nettement moins autonome et eile est située beaucoup plus loin de l'industrie que ce qui avait été prévu à l'origine. Cet article présente trois perspectives distinctes sur l'évolution organisationnelle, affirmant que chacune peut contribuer à la compréhension de l'évolution de l'Agence spatiale canadienne par rapport à sa conception d'origine. Pour étayer cet argument théorique, l'article précise la structure de l'industrie spatiale, les diverses initiatives entreprises pour guider le développement du secteur, ainsi que les circonstances entourant la création de l'agence spatiale. La dernière section de l'article, s'appuyant sur des entrevues avec des fonctionnaires et des intervenants privés, montre pourquoi, lorsqu'ils ont créé l'agence spatiale, certains fonctionnaires, politiciens et représentants de l'industrie ont été incapables de surmonter plusieurs obstacles clés décrits dans l'introduction