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Next‐Generation Sequencing of the Bacterial 16S rRNA Gene for Forensic Soil Comparison: A Feasibility Study
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Ellen M. Jesmok M.S. James M. Hopkins M.S. David R. Foran Ph.D. 《Journal of forensic sciences》2016,61(3):607-617
Soil has the potential to be valuable forensic evidence linking a person or item to a crime scene; however, there is no established soil individualization technique. In this study, the utility of soil bacterial profiling via next‐generation sequencing of the 16S rRNA gene was examined for associating soils with their place of origin. Soil samples were collected from ten diverse and nine similar habitats over time, and within three habitats at various horizontal and vertical distances. Bacterial profiles were analyzed using four methods: abundance charts and nonmetric multidimensional scaling provided simplification and visualization of the massive datasets, potentially aiding in expert testimony, while analysis of similarities and k‐nearest neighbor offered objective statistical comparisons. The vast majority of soil bacterial profiles (95.4%) were classified to their location of origin, highlighting the potential of bacterial profiling via next‐generation sequencing for the forensic analysis of soil samples. 相似文献
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Patricia Hassett 《The Law teacher》2013,47(2):130-144
The University of Western Australia (UWA) has recently undertaken a restructure of all its courses. Under the new courses structure, law at UWA is offered at postgraduate Masters level only, as a three-year professional Juris Doctor (JD) degree rather than as an undergraduate Bachelor of Laws degree (LLB). In planning and preparing for the transition from teaching at an undergraduate Bachelor level to teaching at a postgraduate Masters level, the Law Faculty undertook a major review of its curriculum. An important objective of the review was to ensure that the new JD curriculum satisfies the current learning outcomes standards recently adopted by the Australian regulatory framework. The purpose of this article is twofold. First, the article provides a brief background to the changing legal education environment in Australia in the twenty-first century and considers some of the reasons for the adoption of the JD degree at UWA. Second, the article outlines from a practical perspective the planning, implementation and review processes that have been undertaken by the Faculty in meeting the challenges presented by the transition to the JD degree. The article concludes with a brief comment on the future challenges for the Faculty in implementing and maintaining the renewed curriculum. 相似文献
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ABSTRACTThe study of formal planning in nonprofits and the public sector is thriving, with management gurus providing abundant advice on its value and proper execution. We address a related, but broader issue: why has the management tool of formal planning become prevalent in organizations with a public goal in the first place? To answer this question, we draw on insights from institutional theories of organization, bringing a fresh perspective to the increasingly common practice of formal planning in the administration of public entities. Using a unique dataset constructed from interviews with a random, representative sample of the leaders of 200 nonprofits in the San Francisco Bay Area, we analyze the factors associated with the presence of a formal plan. We combine the interview data with details on organizational characteristics from tax reports and consider the features of nonprofits that plan using logistic regression. The findings reveal that size and capacity are important, but links to an external, rationalized environment dampen the effects of both. Thus, functional factors, while important, are insufficient to explain why nonprofits engage in planning. For those interested in promoting formal planning as a management tool, our findings provide insight into other organizational features that promote the use of planning. And for those concerned with the potentially deleterious effects of this tool in the nonprofit sector, we show that certain types of organizations seem adept at maintaining a less formal structure. 相似文献
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Ping Wu Renee D. Goodwin Cordelia Fuller Xinhua Liu Jonathan S. Comer Patricia Cohen Christina W. Hoven 《Journal of youth and adolescence》2010,39(2):177-188
Using a sample of 781 adolescents (ages 13–17, 52.8% male) from a community survey, this study examined gender differences
in the co-occurrence of specific anxiety disorders with substance use in adolescents. The associations between anxiety disorders
and substance use differed according to the particular anxiety disorders and forms of substance use being examined, as well
as by gender. Social phobia was associated with cigarette smoking among boys only. For girls, social phobia appeared to be
negatively associated with drug use. For the other anxiety disorders, the associations with substance use tended to be stronger
among girls. These findings highlight the need to improve clinical recognition of the anxiety disorders and to improve treatment
access for afflicted adolescents. Future studies based on longitudinal data could further elucidate the relationships among
anxiety disorders, gender, and substance use. 相似文献
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Patricia Hogwood 《German politics》2013,22(3):125-144
For many Germans, citizenship is a problematic and emotive issue, as is demonstrated by the controversies generated around current attempts to modernise and ‘normalise’ German citizenship law. This article looks to German history to account for the failure of successive governments to effect such change. It asserts that two key historical developments have been decisive in stalling a ‘normal’ western European pattern of policy development in this field. First, the concept and law of citizenship in Germany were originally formulated in the context of nation‐state development based on cultural or ‘völkisch’ nationalism. Second, West German governments subordinated the development of citizenship policy to the aim of upholding the West German claim to be the sole legitimate representative of the Germans, thereby denying the legitimacy of the GDR. The unification of Germany in 1990 removed the specific constraints which had brought about the stalled citizenship policy. The article contrasts a Kulturnation (ethno‐cultural) stance on citizenship issues with a Verfassungsnation (civic‐territorial) understanding, and identifies contemporary partisan positions within this conceptual framework. 相似文献