4株猪瘟流行野毒株病毒结构蛋白E2基因的核苷酸序列分析

应用RT-PCR和nPCR扩增由4株甘肃省近期(1997-1998)猪瘟流行野毒株的E2基因,将其克隆到pGEMTEasy载体上,经转化、筛选、鉴定后,测出核苷核序列.4株流行毒株的E2基因核苷酸序列同源性为89.2%-99.7%,相应的氨基酸序列同源性为93.8%-99.0%.这4株流行毒株;与C-株(疫苗种毒)E2基因的核苷酸序列同源性为82.2%-84.3%,相应的氨基酸序列同源性为87.9%-90.2%,表明近期猪瘟流行毒株与C-株的gp55蛋白之间存在一定的差异.     

利用aroA基因建立鸡传染性鼻炎PCR诊断方法

利用aroA基因设计了1对引物,分别对10株标准副鸡嗜血杆菌(HPG)菌株、14株分离的HPG菌株进行PCR扩增,结果均得到了与预期大小一致的片段,而对10株非HPG菌株和3株病毒进行扩增则无相应片段产生;该PCR能检测出10个菌细胞.与常规PCR方法相比,aroA-PCR的敏感性更高.     

The political economy of global firms from India and China

This article attempts to build a bridge between contemporary studies of global firms from emerging economies and existing theories in comparative political economy. It argues that given the primacy of the state as an economic actor in developing countries, the variety of capitalism literature could provide a theoretical foundation for firm-level analyses of emerging market multinationals. For example, the authors suggest that China and India may be moving towards a ‘hybrid market economy’. They also offer a typology of Indian and Chinese corporates to demonstrate an empirical approach to analysing domestic business–government relationships and the ways in which these firms are shaped by the peculiarities of their respective institutional setting. Finally, they identify some of the likely pitfalls of doing cross-national comparisons of emerging market multinationals, particularly with respect to the reliability of corporate data.     

职场友谊对员工职业成长的影响研究 —— 工作旺盛感的中介作用

伴随着组织扁平化和无边界职业生涯时代的到来,工作轮换成为频繁现象,为在劳动力市场中保持持续的竞争优势,关注和促进组织内的职业成长成为人们的最佳选择。本研究以职场友谊为切入点,探索职场友谊对员工职业成长的影响作用,同时探究了工作旺盛感在其中的中介效应。研究结果显示,职场友谊对员工职业成长有显著的正向影响;职场友谊对工作旺盛感有显著的正向影响;工作旺盛感对职业成长有显著的正向影响;工作旺盛感在职场友谊和职业成长的关系中起部分中介作用。在此研究基础上,提出对管理实践的启示,同时进一步探讨了本研究的不足和展望。     

Does China’s middle class prefer (liberal) democracy?

Many surveys show that China’s political regime, under the Chinese Communist Party’s authoritarian rules, enjoys a high level of public support. However, it is still uncertain whether China’s emerging middle class will become the “agent of democratization” as suggested by modernization theory. Using the data of Asian Barometer Survey conducted in China in 2011, this article demonstrates that the relationship between class identity and preference for liberal democracy in China may be inverted U-shaped. The Chinese middle class shows a higher preference to features of liberal democratic regimes than its counterparts of the lower- and upper-class. Members of the Chinese middle class also tend to regard democracy as the best form of government. Thus, the middle class has the potential to initiate democratization in China if the Chinese government fails to keep satisfying the middle class’ quest for economic well-being and protection of property rights.     

The Peer Context and the Development of the Perpetration of Adolescent Dating Violence

The peer context is a central focus in research on adolescent risk behaviors but few studies have investigated the role of the peer context in the perpetration of adolescent dating violence. This longitudinal study examined between-subjects and within-person contemporaneous and lagged effects of peer attributes, measured with social network analyses, on trajectories of dating violence perpetration and determined if effects varied by grade and/or sex of the adolescent. Data are from adolescents who participated in a five-wave panel study beginning when they were in 7 through 9th grade and ending when they were in 10 through 12th grade (n = 3,412); half were male, 40.5 % were white, 49.9 % were black and 10.4 % were of another race/ethnicity. Significant between-subjects effects indicate that adolescents who typically have friends who use dating violence, and girls who are typically high in social status, are at increased risk for using dating violence throughout adolescence. Adolescents who typically have high quality friendships and girls who typically have friends with pro-social beliefs are at decreased risk for using dating violence throughout adolescence. Significant within-person contemporaneous effects indicate that both boys and girls reported lower levels of dating violence than usual at times when they had more friends with pro-social beliefs, and reported higher levels of dating violence than usual at times when they had higher social status. None of the lagged effects were significant and none of the effects varied across grade. These findings suggest that the peer context plays an important role in the development of the perpetration of adolescent dating violence.     

Rethinking public policy in agriculture: lessons from history,distant and recent

This article reviews the histories of agricultural policy in 11 of today's developed countries between the late-nineteenth and the mid-twentieth century and in 10 developing and transition economies since the mid-twentieth century. After discussing the theoretical limitations of the prevailing orthodoxy, the article discusses the history of a wide range of agricultural policies concerning issues like land, knowledge (e.g., research, extension), credit, physical inputs (e.g., irrigation, transport, fertilizers, seeds), farm income stability (e.g., price stabilisation measures, insurances, trade protection), marketing, and processing. The article ends by discussing the policy lessons that may be learned from these historical experiences.     

Effect of Wind on Horizontal Displacement of Fatal Fall from a Height

The horizontal displacement of the human body resulting from fatal fall from a height is an important variable commonly used to inversely determine the cause or identify other forensic aspects of the fall. When examining the horizontal displacement, the wind effect is generally ignored. This technical note reports analytical modeling of the falling process, utilizing previous measurements of wind force acting on the human body, for determining the functional relationship between the wind speed and the horizontal displacement in the falling process. The result reveals that it does not take extremely rare wind conditions to cause a considerable shift of the human body, highlighting the importance to consider wind as a factor in investigations of fatal falls.     

Molecular Identification of Necrophagous Dermestes Species in South Korea Using Cytochrome c Oxidase Subunit I Nucleotide Sequences (Genus Dermestes)

Species identification of necrophagous insects found on a dead body is an essential key in applying medicolegal entomology to the estimation of postmortem interval (PMI). Due to limited morphological identification of insect evidence, several studies have identified species using molecular information such as DNA markers. While considerable cytochrome c oxidase subunit I (COI) gene sequence data of necrophagous fly species have been collected and annotated, those of necrophagous beetle species have not. Since necrophagous beetles such as Dermestes species have a larval period longer than that of flies, beetles are useful in even the late decomposition phase in estimating minimum PMI. To obtain the full-length COI gene sequences of six Dermestes species collected from South Korea, we designed primers for polymerase chain reaction amplification and sequencing. The obtained full COI nucleotide sequences were used for performing phylogenic analysis and comparison with previously reported sequences. The results demonstrated that the COI gene sequences could be used to identify forensically important Dermestes species in South Korea.     

Correction to: Age Patterns in Risk Taking Across the World

Journal of Youth and Adolescence - In the original publication, the legends for Figs 4 and 5 were incorrect, such that each regression line was mislabeled with the incorrect country. Below are the...