Rapid determination of ketamine in urine by liquid chromatography-tandem mass spectrometry for a high throughput laboratory

In recent years, the abuse of ketamine had gained popularity in rave parties in Hong Kong. The Urinalysis Unit of the Government Laboratory of Hong Kong faced a tremendous increase in workload for ketamine analysis. The number of tests performed rose from 10 in 1999 to 15,000 in 2002. As a fully validated immunoassay test for ketamine was not available in the market, most laboratories analyzed ketamine by chromatographic techniques after liquid-liquid extraction. However, these methods reported in the literature are not suitable for high throughput laboratories. Hence, a rapid screening/confirmation method for ketamine by liquid chromatography-tandem mass spectrometry (LC-MS-MS) with a detection limit of 5 ng/ml was developed. After automated solid-phase extraction (SPE), the urine extract was analyzed for ketamine by a 2.5-min chromatographic run, the estimated recovery was 89% and the precision was 11% R.S.D. at 20 ng/ml. With the aid of an in-house developed computer program, the results were presented in spreadsheet format for easy checking. The method has been applied to our laboratory for routine ketamine analysis and a maximum of 200 samples per day can be achieved.     

Patterns and Dynamics of China's International Strategic Behaviour

The acceptance and promotion of partnerships among major countries reflect an attempt on the part of China to re-define its position in the new international strategic pattern. They also reveal a strategic idea offered by China in the post-Cold War era in which it is emerging as a major power. The key questions are as follows. (a) What are the contents of such partnerships among major powers? (b) What are the differences between this partnership model among major powers and the Chinese foreign policy of independence and peace in the 1980s? (c) What are the motivations behind China's promotion of this model of partnerships among major powers? (d) Does the new model of partnerships among the major powers provide an important innovation in the management of relations among the major powers in the coming century? This article attempts to answer these questions so as to better understand China's international strategic behaviour patterns in the post-Cold War era, the inner logic of partnership relationships, and the trends of China's international strategy in the coming century.     

贿赂罪共同犯罪问题研究

贿赂罪共同犯罪问题 ,是研究贪污贿赂犯罪中不可回避之重要刑法理论问题 ,其不仅作为典型的共同犯罪问题而倍受刑法共同犯罪理论研究的广泛关注 ,而且对于当前惩治腐败 ,加强廉政建设 ,具有重要的现实意义。非国家工作人员能构成受贿罪共犯 ;单位也能作为贿赂罪共同犯罪的主体     

Expression of TGF-beta 1 and proliferation of collagen in myocardium in viral myocarditis

To investigate the tissue repair of acute viral myocarditis, and to explore the diagnostic method of slight viral myocarditis in forensic pathology. Slight viral myocarditis model was induced in Balb/c murine by CVB3. Collagen proliferation in myocardium of mice with myocarditis was observed by special staining. The hearts of mice and human(9 cases) with myocarditis were studied LSAB-immunohistochemically with anti-TGF-beta 1 antibody. In the study, the proliferation of collagen was seen in myocardium in acute viral myocarditis. Generous expression of TGF-beta 1 was found in the myocardium of mice and human with myocarditis. The quantity of collagen proliferation and expression of TGF-beta 1 was positive correlation. It is concluded that the tissue repair exists in acute viral myocarditis and that positive staining of myocardium for TGF-beta 1 is a sensitive index of myocardial damage and tissue repair.     

Photoluminescent semiconductor nanocrystals for fingerprint detection

The concept of utilizing photoluminescent semiconductor nanocrystals for latent fingerprint detection, especially in concert with phase-resolved imaging for background fluorescence suppression, is reduced to practice with CdS nanocrystals that are capped with dioctyl sulfosuccinate. The nanocrystals are dissolved in heptane or hexane and are applied in much the same way as staining with fluorescent dye, on articles that have been pre-fumed with cyanoacrylate ester and also on the sticky side of electrical tape without pre-fuming. Since CdS can form a photoluminescent nanocomposite with dendrimers, a feasibility examination of dendrimer tagging of fingerprints has also been conducted.     

根据中国汉族男性甲状软骨推算年龄的研究

目的根据中国汉族男性甲状软骨推算年龄。方法对73例已知年龄的中国汉族男性甲状软骨进行软X线摄片和(或)X线摄片,从摄片上选择8个与年龄变化关系较为密切的观察部位,每个部位又划分成3至6个等级,然后采用多元线性回归方法,建立其推算年龄的数学模型。结果运用该方法推算男性甲状软骨年龄,其标准差为1.354,复相关系数R为0.9971。结论本方法适用于中国汉族男性甲状软骨的年龄推算,其有效力年龄范围为13-67岁。     

包惠僧参加一大的身份考证——兼谈包惠僧的资格问题

包惠僧参加了"一大",但其代表资格问题一直成为党史界争论的焦点:一种观点认为他不是"一大"代表,"一大"代表数是12人;另一观点认为他是"一大"正式代表,因而"一大"代表数是13人,该观点又有三种看法:代表武汉共产主义小组;代表广东共产主义小组;代表陈独秀.本文通过考证广东共产主义小组的选派过程认为:由于陈独秀等对"一大"不重视,广东共产主义小组只派陈公博一人作为代表出席"一大",陈独秀托其捎去致大会的信;在通知陈公博后的较长时间广东发生一些变化,陈独秀又临时指派包惠僧前往上海解释说明,包惠僧的这种特殊身份使其不是代表而得以进入会场参加大会,与会者对其资格有不同的理解,才有"正式代表说"和"非正式代表说".决定其资格问题的关键是身份.包惠僧只是陈独秀派往上海向临时中央汇报情况的使者而非"一大"代表,包惠僧不具备"一大"代表资格.     

论制度正义与道德自觉

制度与制度正义是当今学术界诸多学科关照与纷争不断的主题 ,如何对当下热烈的学术论争作理性与深层的哲学审视 ,如何将制度正义贯穿于社会发展的全过程 ,尤其是探讨道德自觉与制度正义的关系 ,对正处转型时期的我国具有特殊意义。以历史性的眼光解读社会结构亦即公共权力运作体制的正义性 ,并以之指导我国政治体制改革的路径选择。     

略论五四时期的东方文化思潮

五四时期的东方文化思潮肇端于“中体西用”论,国粹主义是其重要的思想资源。时代的焦虑和“西方的没落”使得东方文化思潮应运而起。出于对中国近代所走过的西化道路模式的痛切反省,它主张以东方文化之长解西方文化之弊,力图为中国寻求一条超越西方模式的健全的近代化道路。虽然东方文化思潮有着内在理论缺陷,但它在谋求中国传统文化向近代化的转型方面作出了有益的探索和宝贵的贡献。