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101.
Claire E.V. French Cynthia G. Jensen Susan K. Vintiner Douglas A. Elliot Susan R. McGlashan 《Forensic Science International Supplement Series》2008,178(1):1-6
There are a number of forensic cases in which the identification of the epithelial cell type from which DNA originated would provide important probative evidence. This study aimed to develop a technique using histological staining of fixed cells to distinguish between skin, buccal and vaginal epithelium. First, 11 different stains were screened on formalin-fixed, wax-embedded cells from five women. Samples were analysed qualitatively by examining staining patterns (colour) and morphology (absence or presence of nuclei). Three of the staining methods – Dane's, Csaba's and Ayoub-Shklar – were successful in distinguishing skin epithelial cells from buccal and vaginal. Second, cells were smeared directly onto slides, fixed with one of five fixatives and stained with one of the three stains mentioned above. Methanol fixation, coupled with the Dane's staining method, specific to keratin, was the only technique that distinguished between all three cell types. Skin cells stained magenta, red and orange and lacked nuclei; buccal cells stained predominantly orange–pink with red nuclei; while vaginal cells stained bright orange with orange nuclei and a blue extracellular hue. This staining pattern in vaginal cells was consistent in samples collected from 50 women aged between 18 and 67. Identification of cell type from unlabelled micrographs by 10 trained observers showed a mean success rate of 95%. The results of this study demonstrate that histological staining may provide forensic scientists with a technique for distinguishing between skin, buccal and vaginal epithelial cells and thus would enable more conclusive analyses when investigating sexual assault cases. 相似文献
102.
Managing incidental findings in human subjects research: analysis and recommendations. 总被引:8,自引:8,他引:0
Susan M Wolf Frances P Lawrenz Charles A Nelson Jeffrey P Kahn Mildred K Cho Ellen Wright Clayton Joel G Fletcher Michael K Georgieff Dale Hammerschmidt Kathy Hudson Judy Illes Vivek Kapur Moira A Keane Barbara A Koenig Bonnie S Leroy Elizabeth G McFarland Jordan Paradise Lisa S Parker Sharon F Terry Brian Van Ness Benjamin S Wilfond 《The Journal of law, medicine & ethics》2008,36(2):219-48, 211
No consensus yet exists on how to handle incidental findings (IFs) in human subjects research. Yet empirical studies document IFs in a wide range of research studies, where IFs are findings beyond the aims of the study that are of potential health or reproductive importance to the individual research participant. This paper reports recommendations of a two-year project group funded by NIH to study how to manage IFs in genetic and genomic research, as well as imaging research. We conclude that researchers have an obligation to address the possibility of discovering IFs in their protocol and communications with the IRB, and in their consent forms and communications with research participants. Researchers should establish a pathway for handling IFs and communicate that to the IRB and research participants. We recommend a pathway and categorize IFs into those that must be disclosed to research participants, those that may be disclosed, and those that should not be disclosed. 相似文献
103.
In an effort to take positive steps toward coping with problems for families and children created by high levels of separation and divorce, ever increasing civil caseloads and the exposure of children to interparental conflict, court‐affiliated educational programs have emerged in the United States for parents separating from their spouse or partner or going through a divorce. This article will provide an overview of the creation of such programs and their development, which includes a discussion regarding the numerous states currently mandating parents to attend. It will summarize some of the research which has been conducted as to the efficacy of the programs and will provide the results of our nationwide research for each state's parent education status. There is a discussion of domestic violence issues and sensitivities in the context of parent education programs and possible future directions for mandatory parent education. 相似文献
104.
105.
106.
Susan Eckstein 《拉美政治与社会》2009,51(1):119-148
This article documents a U.S. Cuban foreign policy cycle that operated in tandem with the presidential electoral cycle between 1992 and 2004. During these post–Cold War years, when Cuba posed no threat to U.S. national security, influential, well-organized Cuban Americans leveraged political contributions and votes to tighten the embargo on travel and trade, especially at the personal level. U.S. presidential candidates, most notably incumbent presidents seeking re-election, responded to their demands with discretionary powers of office. When presidential candidates supported policies that made good electoral sense but conflicted with concerns of state, they subsequently reversed or left unimplemented Cuba initiatives. After describing the logic behind an ethnic electoral policy cycle and U.S. personal embargo policy between 1992 and 2004, this article examines Cuban American voter participation, political and policy preferences, lobbying, political contributions, and the relationship between the ethnic policy and presidential election cycles. 相似文献
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108.
Courts resolving child support cases involving separated, divorced, and non‐marital children are charged with defining responsibility for health care coverage for the children under that order. This article explores historical and current medical child support requirements under Title IV‐D of the Social Security Act—the national child support enforcement (“IV‐D”) program. It analyzes legal requirements and policy recommendations, and provides a practical tool judges may use to determine whether health care coverage available to either or both parents is appropriate—that is, comprehensive, accessible, and affordable. 相似文献
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110.
This article proposes bottom-up learning as a normative framework for international NGOs. It explores the common but often unacknowledged disparity between organisational values and mission versus actual practice. The first section of the paper raises the question of organisational learning disorders followed by an exploration of learning organisations and bottom-up learning in particular. A section briefly summarising positive developments in the field is followed by discussions of organisational barriers and possible mitigation techniques. The paper closes with a challenge for international NGOs to take a closer look at their learning capabilities with a view to improving service to communities in need. 相似文献