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Jaime Solano Leonardo Anabalón Sylvia Figueroa Cristian Lizama Luis Chávez Reyes David Gangitano 《Science & justice》2019,59(1):102-108
In nature, there are >200 species of fungi with hallucinogenic properties. These fungi are classified as Psilocybe, Gymnopilus, and Panaeolus which contain active principles with hallucinogenic properties such as ibotenic acid, psilocybin, psilocin, or baeocystin. In Chile, fungi seizures are mainly of mature specimens or spores. However, clandestine laboratories have been found that process fungus samples at the mycelium stage. In this transient stage of growth (mycelium), traditional taxonomic identification is not feasible, making it necessary to develop a new method of study.Currently, DNA analysis is the only reliable method that can be used as an identification tool for the purposes of supporting evidence, due to the high variability of DNA between species. One way to identify the species of a distinctive DNA fragment is to study PCR products analyzed by real time PCR and sequencing. One of the most popular sequencing methods of forensic interest at the generic and intra-generic levels in plants is internal transcribed spacer (ITS). With real time PCR it is possible to distinguish PCR products by differential analysis of their melting temperature (Tm) curves.This paper describes morphological, chemical, and genetic analysis of mycelia of psychedelic fungi collected from a clandestine laboratory. The fungus species were identified using scanning electron microscopy (SEM), mass spectrometry, HRM analysis, and ITS sequencing. The sporological studies showed a generally smooth surface and oval shape, with maximum length 10.1?μm and width 6.4?μm. The alkaloid Psilocyn was identified by mass spectrometry, while HRM analysis and ITS sequencing identified the species as Psilocybe cubensis. A genetic match was confirmed between the HRM curves obtained from the mycelia (evidence) and biological tissue extracted from the fruiting bodies. Mycelia recovered from the evidence and fruiting bodies (control) were genetically indistinguishable. 相似文献
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Family violence is a core social issue in every country around the world. The extent and consequence of family violence as a major problem has received increased attention around the world. The focus of the present study is not only to inform about intimate partner violence (IPV) that occurs in individual countries, but to illustrate how individuals, families, communities, and cultures use their strengths to overcome the challenges that violence presents. An analysis of 16 countries, including 17 cultures is represented and includes all seven of the world’s major geocultural areas. Qualitative methodology was used to examine the stories of intimate partner violence in which similarities and differences across cultures were discovered resulting in a theoretical discussion of findings. The International Family Strengths Model was applied to examine the family strengths, community strengths, and cultural strengths used to mitigate family violence worldwide. Individual strengths emerged as a new and important component of the model. Suggestions for change include recognition of differing solutions across cultures, the need for mediation and policy changes, and the importance of empowerment. 相似文献
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Sylvia Bawa 《国际公共行政管理杂志》2013,36(4):282-291
This article discusses empirical findings on issues affecting women's effective participation in politics and the public sector of Ghana. It argues that women's involvement in public life and politics has steadily declined contrary to hopeful reportage that global empowerment campaigns have increased women's political participation across Africa. The article suggests that given the deep-rooted socio-cultural hindrances women face, affirmative action policies need to be revisited to complement women's agency in contesting for, winning, and participating in politics and public service more effectively. 相似文献
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Because of the absence of evidence to show for its utility, the notion of ‘development’ has been fraught with many debates over the years. This paper is concerned with re-examining the future of development studies, based on its past and present trajectories. The argument here is that development may be useful if its norms and practices become context-specific and are made to benefit its purported beneficiaries. The chronology spans the period after World War II to the present day, and thus covers theories that envision alternatives. While this chronology is overlapping, we hope to show that development studies has been marked by both continuities and discontinuities. 相似文献
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Sylvia Alvarado 《美中公共管理》2014,(12):995-1004
This paper performs an analysis of the optimum currency area (OCA) for ASEAN (Association of Southeast Asian Nations) and ASEAN+3 for the period of 2003-2012. The applied method is based on the model developed by Bayoumi and Eichengreen for the European countries. Regarding the two groups of study, the document contents the integration roots as well as some theories and empirical data about the currency areas. The obtained results based on the OCA indexes showed that nearly half of the country members have moved symmetrically, although the effect of the four independent variables was found different in each case. In conclusion, the monetary convergence is significantly influenced by the output disturbances and the trade linkages in both regions; while the size of the economy only becomes significant in ASEAN+3 and the synchronic advantage is not contributing and even insignificant for ASEAN+3. 相似文献
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Sylvia KierkegaardAuthor Vitae 《Computer Law & Security Report》2011,27(3):278-290
In an unprecedented legal development, the case of violence in video games has now reached the highest American court. The US Supreme Court is set to decide whether states can restrict minors from buying violent video games in the case of Schwarzenegger v. Entertainment Merchants Association. The decision could have serious implications on the future of First Amendment rights and children’s ’welfare. To resolve Schwarzenegger, the Justices will need to decide how much First Amendment protection should be extended to violent video games and whether minors have a greater constitutional right to violence than they do to obscenity. 相似文献