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C. Haas E. HansonM.J. Anjos W. BärR. Banemann A. BertiE. Borges C. BouakazeA. Carracedo M. CarvalhoV. Castella A. ChomaG. De Cock M. DötschP. Hoff-Olsen P. JohansenF. Kohlmeier P.A. LindenberghB. Ludes O. MaroñasD. Moore M.-L. MorerodN. Morling H. NiederstätterF. Noel W. ParsonG. Patel C. PopielarzE. Salata P.M. SchneiderT. Sijen B. Svie?enaM. Turanská L. ZatkalíkováJ. Ballantyne 《Forensic Science International: Genetics Supplement Series》2012,6(1):70-80
A second collaborative exercise on RNA/DNA co-analysis for body fluid identification and STR profiling was organized by the European DNA Profiling Group (EDNAP). Six human blood stains, two blood dilution series (5-0.001 μl blood) and, optionally, bona fide or mock casework samples of human or non-human origin were analyzed by the participating laboratories using a RNA/DNA co-extraction or solely RNA extraction method. Two novel mRNA multiplexes were used for the identification of blood: a highly sensitive duplex (HBA, HBB) and a moderately sensitive pentaplex (ALAS2, CD3G, ANK1, SPTB and PBGD). The laboratories used different chemistries and instrumentation. All of the 18 participating laboratories were able to successfully isolate and detect mRNA in dried blood stains. Thirteen laboratories simultaneously extracted RNA and DNA from individual stains and were able to utilize mRNA profiling to confirm the presence of blood and to obtain autosomal STR profiles from the blood stain donors. The positive identification of blood and good quality DNA profiles were also obtained from old and compromised casework samples. The method proved to be reproducible and sensitive using different analysis strategies. The results of this collaborative exercise involving a RNA/DNA co-extraction strategy support the potential use of an mRNA based system for the identification of blood in forensic casework that is compatible with current DNA analysis methodology. 相似文献
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Deena Patel 《Family Court Review》2005,43(1):164-177
Morbid obesity is an unfortunate problem that is only becoming worse everyday. The alarming aspect of it is that it is affecting people at a much earlier age; thus, young children are becoming morbidly obese and are experiencing the same health problems as middle-aged adults. The first section of this note defines morbid obesity and its causes and trends. Section two describes child neglect in general and then distinguishes medical neglect. Section three discusses education and prevention, which are both crucial steps in the struggle with weight-loss and weight-gain. Section four summarizes the beneficial aspects of removing a morbidly obese child who is in a life-threatening position from his or her parents. Section five highlights case-studies from California, Iowa, Indiana, New Mexico, and Texas, where children have either died or were removed from their families because of morbid obesity. Section six briefly touches upon similar child neglect scenarios where removal is permissible and compares those situations with those of morbidly obese children. The seventh section considers some credible counterarguments to government intervention, and the final section suggests some recommendations on how to prevent the morbid obesity crisis from becoming worse than it already is. Too many people are dying from weight-related problems already, yet morbid obesity is preventable! This severe state of obesity is something that can be controlled and prevented, but only if parents take an active role in their child's diet and exercise. 相似文献
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F Patel 《Forensic science international》1992,56(2):183-188
A baffling case of fall-from-height is described focusing on aspects of a human body passing through a small hole, within a holeproof window. It is a classic example of an unsatisfactory outcome when a scene of death is modified adversely due to delay in the commencement of scene management. The operative factors may be entirely outside the control of scene investigators. The primary medical attendant is reminded of the forensic obligations at a scene of unnatural death. Reporting this case might encourage forensic practitioners having experience of a similar case to respond through this journal. 相似文献
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A H Stead J Watton C P Goddard A C Patel A C Moffat 《Forensic science international》1986,32(1):49-60
A radioimmunoassay (RIA) has been developed for the direct detection of LSD in biological fluids. The radiotracer, (+)-2-[125I]iodo-LSD, allows the use of gamma-counting rather than the liquid scintillation counting currently used for existing 3H radioimmunoassays. The assay is specific for LSD and very closely related compounds. It is inexpensive, sensitive, simple to use and small volumes of samples (50 microliter) can be assayed directly without the need for any time-consuming extraction procedures. The cut-off levels are 1.2 ng/ml in blood and 3.0 ng/ml in urine. The results obtained using the 125I assay described in this work compare very favourably with those obtained using the 3H assay currently used by Home Office Forensic Science Laboratories. The advantages of the assay make it a most appropriate method for the routine screening of LSD in biological samples of forensic interest. 相似文献
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We come to an analysis of Third Worldism through an historical understanding of the development project, one that locates Third Worldism as a moment in a broader series of resistances both to capital and colonialism, and to the techniques used by the state to maintain hegemony. Viewing Third Worldism in this wider context, we argue, enables us to not only explain the failure of Third Worldism to deliver on its vision of emancipation from colonialism, but to also explain the shape of contemporary resistance to the world capitalist order. We argue that the theory and practice of development depends on a certain biopolitics, rooted in a regime of sovereign state control, and designed to mobilise citizens in ways favourable to capital. We hold that Third Worldism embraced this form of sovereignty and its biopolitics. Further, by blending cultural studies analysis with a Polanyian interpretation of the rise of fascism, we argue that Third Worldism can be situated as a moment in the maturation of ‘global fascism’. Finally, we argue that contemporary resistances to neoliberalism have recognised the complicity of the state with capital. These ‘new internationalisms’ arise from the ashes of Third Worldism, with an altered understanding of ‘sovereignty’ that challenges the trajectory of the Third World sovereign state. 相似文献
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Nilay B. Patel 《Commonwealth Law Bulletin》2013,39(4):559-568
If there was ever a case where the factual substratum could not have ever been foreseen, McCully v Whangamata Marina Society Inc & Anor 1 (McCully) was one. The case is an unexpected by‐product of the substantive underlying case of Whangamata Marina Society Inc v Attorney‐General 2 (Whangamata) where the member of Parliament (MP) was not a party. The McCully case is unusual because: (1) it is, in law, a civil procedure case that matures into a significant constitutional law case; and (2) it is not the ruling alone, but the factual substratum particularly, that touches on the very heart of constitutional law. This article is limited to a cross‐analysis of the separation of powers, the sub judice rule, and ministerial decision‐making. 相似文献