新生大鼠大脑皮质神经元原代培养方法的建立及其活性鉴定

采用倒置相差显微镜、环境扫描电子显微镜对新生24 h内SD大鼠大脑皮质原代培养的神经元进行了形态学观察,建立了大脑皮质神经元原代培养方法。应用2.5μg/mL阿糖胞苷(Ara-C)对培养3 d的神经元进行24 h干预,去除神经胶质细胞、成纤维细胞、神经干细胞等杂质细胞,以提高神经元产率;用Nissl’s染色法进行神经元鉴定;利用微量滴定(MTT)法测定了一个培养周期(约10 d)的神经元活性分布。光学显微镜观察结果表明,培养至第4 h绝大多数神经元已贴壁生长,胞体上可见1~2个细小突起;培养至第3 d、第6 d时,Nissl’s染色结果显示,加Ara-C能显著提高神经元纯度(≥92%);MTT结合形态学观察结果表明,神经元培养至第6 d时活性最强,细胞呈卵圆形、蜘蛛状、锥体状等多种形态,细胞胞体饱满,光晕明显,神经网络形成完整。     

广西与东盟高校之间的合作与交流——对人才培养模式、专业与课程设置的探讨分析

一、引言自从中国与东盟签署了《中国—东盟全面经济合作框架协议》,决定到2010年建成中国—东盟自由贸易区,就为双方全方位合作带来广阔的前景。广西因其地理位置而成为中国—东盟自由贸易区的“桥头堡”,且我国在加入W TO后,教育的对外开放得到了进一步扩大,广西地处西部地区,又有国家给予的相对优惠的政策,因此,广西必须抓住机遇,迎接挑战。“科学技术是第一生产力”,在全球经济一体化的趋势下,发展高新技术产业越来越成为各国经济发展的重点,而科技又是以教育,尤其是高等教育为重心的。高等教育是所有国家,不论是发达国家还是发展中国…     

China-U. S. Economic and Trade Relations

Sino-U. S. economic and trade relations as the most important and dynamic component of bilateral ties have evolved into the stabilizer, pillar and driving force for cementing the linkage between the two capitals. Their furtherance along the designated line of treating a "stake holder" and handling a "constructive partnership" will carry an ever-ballooning strategic significance even in diplomatic and security terms. Nevertheless the general trend looks set to be incessant progress amidst reciprocal accommodation in disregard of occasional twists and turns.     

第二性,民主——关于民主地位的反思

民主在中国备受关注,对民主地位的理解常有对立。本文基于对中国现当代历史事实的反思,循唯物史观原理,论证了"第二性,民主"。提出:民族高于民主,民生高于民主,发展高于民主。民主进步的合理性不能仅由民主自身定,而应置于社会整体发展的参照系中观察。中国共产党提出"坚持党的领导、人民当家作主和依法治国有机统一",是中国现代化进程中民主政治建设的绩优框架,它是中国经验的总结,也是对西方民主理念进行"颠覆"性"落实"的后现代思维。     

Expand Friendly Cooperation and Build a Harmonious World

As early as in the beginning of the 1980's,the Chinese Association for International Understanding (CAFIU) started exchanges with the Friendrich Ebert Stiftung (FES). Since FES set up its Beijing office in 1987,fruitful cooperation has been conducted between FES and CAFIU,and other relevant parties of the Chinese side. Over the years,FES has made positive efforts and great contributions for deepening and developing understanding and friendship between the Chinese people and the German people,and for promoting the relations between the two countries and two parties,which we highly appreciate.     

“事功”与“格物”:高校学报的现状及定位

“学术失范”是指学术界的某些方面存在着缺乏规范,或有规不依或规范本身不合理,从而贻害学术事业的现象。一定程度上的学术失范引发了部分学术期刊的“失范”。对于高校学报而言,“学术性”“特色性”是其生命力、竞争力所在。     

消除财富歧视实现共同富裕

全面建设小康社会的内容是多方面的,而共同富裕具有决定性的作用。实现共同富裕要坚持效率优先兼顾公平,要提倡创造性劳动,重视资本的合法性,培养纳税人意识,建设趋向共同富裕的机制。     

论当代中国社会公德建设的重要性与紧迫性

社会公德是指在社会交往和公共生活中公民应该遵守的道德准则,它是构建和谐社会的伦理基石,对社会整体文明的发展具有促进作用。在改革开放新时期,我国社会公德状况既有可喜的一面,也有令人堪忧的一面。全面把握社会公德的价值,深入洞察社会公德的现状,是我们充分认识社会公德建设重要性与紧迫性的关键,也是我们自觉、有效推进社会公德建设的前提。     

Juvenile Criminality And Semi-Criminality: Learning From Victorian Perceptions And Responses

This article focuses on media reportage of offensive juveniles, past and present, to elicit lessons that the twenty-first century can learn from the Victorian past in terms of diversionary responses. How to prevent vulnerable juveniles sliding into dangerous criminality is a continuing preoccupation: the issue explored in this article relates to the creation of the identity of the criminal juvenile. In utilising the concept of semi-criminality to label certain types of juvenile anti-social behaviour the Victorians avoided actual criminalisation of socially offensive but, in legal terms, minor behaviours. The reasons for and negative consequences of the abandonment of this concept by the modern age are explored, including the reconceptualisation of where responsibility for juvenile offending lies in the modern era.