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981.
982.
Urine is often the sample of choice for drug screening in aviation/general forensic toxicology and in workplace drug testing. In some instances, the origin of the submitted samples may be challenged because of the medicolegal and socioeconomic consequences of a positive drug test. Methods for individualization of biological samples have reached a new boundary with the application of the polymerase chain reaction (PCR) in DNA profiling, but a successful characterization of the urine specimens depends on the quantity and quality of DNA present in the samples. Therefore, the present study investigated the influence of storage conditions, sample volume, concentration modes, extraction procedures, and chemical preservations on the quantity of DNA recovered, as well as the success rate of PCR-based genotyping for DQA1 and PM loci in urine. Urine specimens from male and female volunteers were divided and stored at various temperatures for up to 30 days. The results suggested that sample purification by dialfiltration, using 3000-100,000 molecular weight cut-off filters, did not enhance DNA recovery and typing rate as compared with simple centrifugation procedures. Extraction of urinary DNA by the organic method and by the resin method gave comparable typing results. Larger sample volume yielded a higher amount of DNA, but the typing rates were not affected for sample volumes between 1 and 5 ml. The quantifiable amounts of DNA present were found to be greater in female (14-200 ng/ml) than in male (4-60 ng/ml) samples and decreased with the elapsed time under both room temperature (RT) and frozen storage. Typing of the male samples also demonstrated that RT storage samples produced significantly higher success rates than that of frozen samples, while there was only marginal difference in the DNA typing rates among the conditions tested using female samples. Successful assignment of DQA1 + PM genotype was achieved for all samples of fresh urine, independent of gender, starting sample volume, or concentration method. Preservation by 0.25% sodium azide was acceptable for sample storage at 4 degrees C during a period of 30 days. For longer storage duration, freezing at -70 degrees C may be more appropriate. Thus, the applicability of the DQA1 + PM typing was clearly demonstrated for individualization of urine samples. 相似文献
983.
When a person is wounded in Turkey, he first attends hospital for treatment. The hospital is responsible for a report describing his injuries and their treatment and prognosis. The patient is then scanned by a specialist in forensic medicine who provides a final official report. In that report the lesions, the prognosis (including whether the injuries are life threatening or not) and the projected days away from daily activity are shown. In this study 18,317 cases which were examined in the second and the third Specialization Board of the Council of Forensic Medicine during 1996 were analyzed. Among them 112 cases were dental injuries. Dental injuries are reviewed according to their sex, causation, detail of injury, and they are compared to other studies. 相似文献
984.
The purpose of this work was to evaluate if it was possible to measure the alcohol concentration in breath by a multisensor array, i.e. an electronic nose. The most important aspects were to clarify technical advantages and disadvantages and if the technique is at all suitable for forensic breath alcohol analysis. Even though the system set-up was far from optimal it is clear that it was possible to quantify breath alcohol with a best-case root mean square error = 16.8 mol ppm ethanol (= 0.037 mg/l). However, the method needs significant development especially of the sensor devices. The sensor array was composed of ten metal oxide silicon field effect transistors (MOSFET) with various catalytic gates and one infrared-based CO2 sensor. The system was evaluated by monitoring the breath ethanol concentration of five test persons after intake of alcohol. Gas chromatography was used in parallel to measure the actual breath alcohol concentration. Different data evaluation techniques applied were projection to latent structure (PLS) and artificial neural networks (ANN). 相似文献
985.
ABSTRACTInstitutions undertake a huge variety of constitutive purposes. One of the roles of legitimacy is to protect and promote an institution’s pursuit of its purpose; state legitimacy is generally understood as the right to rule, for example. When considering legitimacy beyond the state, we have to take account of how differences in purposes change legitimacy. I focus in particular on how differences in purpose matter for the stringency of the standards that an institution must meet in order to be legitimate. An important characteristic of an institution’s purpose is its deontic status, i.e. whether it is morally impermissible, merely permissible, or mandatory. Although this matters, it does so in some non-obvious ways; the mere fact of a morally impermissible purpose is not necessarily delegitimating, for example. I also consider the problem of conflicting, multiple, and contested institutional purposes, and the different theoretical roles for institutional purpose. Understanding how differences in purpose matter for an institution’s legitimacy is one part of the broader project of theorizing institutional legitimacy in the many contexts beyond the traditional context of the state. 相似文献
986.
987.
AbstractThe analysis of issue politics has long suffered from a fragmentation between valence and positional conceptualisations, preventing the effective development of a general model of issue-based party competition. Building on an overview of the evolution of party competition in the Western world in recent decades, this article offers a theoretical development that builds on ‘issue yield’ theory to provide a conceptualisation of political goals that generalises across positional and valence issues. This in turn allows a common measurement strategy, offering the possibility to comparatively assess various characteristics (including the electoral potential) of both positional and valence issues. Finally, it describes the specific research design derived from this framework and its implementation in comparative perspective in six West European countries during 2017–2018. 相似文献
988.
R. J. Mourits N. van den Berg M. Rodríguez-Girondo K. Mandemakers P. E. Slagboom M. Beekman 《The History of the Family》2020,25(3):484-526
ABSTRACT Studies have shown that long-lived individuals seem to pass their survival advantage on to their offspring. Offspring of long-lived parents had a lifelong survival advantage over individuals without long-lived parents, making them more likely to become long-lived themselves. We test whether the survival advantage enjoyed by offspring of long-lived individuals is explained by environmental factors. 101,577 individuals from 16,905 families in the 1812–1886 Zeeland cohort were followed over time. To prevent that certain families were overrepresented in our data, disjoint family trees were selected. Offspring was included if the age at death of both parents was known. Our analyses show that multiple familial resources are associated with survival within the first 5 years of life, with stronger maternal than paternal effects. However, between ages 5 and 100 both parents contribute equally to offspring’s survival chances. After age 5, offspring of long-lived fathers and long-lived mothers had a 16-19% lower chance of dying at any given point in time than individuals without long-lived parents. This survival advantage is most likely genetic in nature, as it could not be explained by other, tested familial resources and is transmitted equally by fathers and mothers. 相似文献
989.
Karel Arnaut Nadia Fadil Jérémy Mandin Jaafar Alloul 《Journal of immigrant & refugee studies》2020,18(3):261-269
AbstractThis article addresses conceptual issues around contemporary forms of out-of-Europe migrations of various European publics. In particular, we ask how such moves contribute to both the decentring of Europe in migration debates, and to the ‘de-migranticization' (Dahinden, 2016) of the social scientific study of migration. This article also serves as the introduction to this special issue entitled “Leaving Europe: Alternative routes of up/outward mobility” that aims to document ethnographically new forms of European emigration and shed light on an imaginary of Europe which is not conceived as a place of prosperity and success but rather as a locus of disempowerment. 相似文献
990.
Sandra Brée 《The History of the Family》2020,25(3):345-368
ABSTRACT Following the law of 1884 that re-authorised divorce in France, divorce was more frequent in the large cities before spreading to other urban areas and then to rural ones. Divorce rates were especially high in the Seine département from 1884 to the eve of the First World War. In this region, divorced people lived more frequently in Paris than in the suburbs. More precisely, they resided more often on the Right Bank of the Seine in Paris proper (intra-muros) and, as an extension of this area, in the suburban cities to the west and, to the east, around the Bois de Vincennes. A comparison of the share of divorced men and women to the economic, demographic and cultural characteristics of the 20 Paris arrondissements and 74 suburban municipalities in the Seine département shows that a portion of the spatial distribution can be explained by the occupational structures of the area. Divorced people seldom lived in the most rural areas. This urban/rural divide can be explained by two main factors that can be complementary: the economic possibility to divorce – and here we will add the economic possibility for women to leave live alone after a divorce – and the social and cultural acceptance of divorce. 相似文献