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11.
Salas A Prieto L Montesino M Albarrán C Arroyo E Paredes-Herrera MR Di Lonardo AM Doutremepuich C Fernández-Fernández I de la Vega AG Alves C López CM López-Soto M Lorente JA Picornell A Espinheira RM Hernández A Palacio AM Espinoza M Yunis JJ Pérez-Lezaun A Pestano JJ Carril JC Corach D Vide MC Alvarez-Iglesias V Pinheiro MF Whittle MR Brehm A Gómez J 《Forensic science international》2005,150(2-3):191-198
A qualitative and quantitative analytical method was developed and validated for the determination of 49 licit and illicit drugs in oral fluid. Small oral fluid samples, volume 1mL, were collected from volunteers using a modified Omni-Sal device and the analytes were extracted from an oral fluid/buffer mixture using a single Bond Elut Certify solid phase extraction cartridge. Liquid chromatography-tandem mass spectrometry (LC-MS-MS) and gas chromatography-repetitive full scan mass spectrometry (GC-MS) were used in parallel to analyze the extracts for the targeted drugs. Extracts were analyzed by GC-MS in their underivatized form and as their pentafluoropropionyl derivatives. Deuterated internal standards were used for quantification of drugs of abuse by LC-MS-MS to minimize matrix effects. Methadone-d(9) and tumoxetine were used as the internal standards for quantification of non-derivatized and derivatized analytes respectively by GC-MS. Linearity was demonstrated over the range 5-200 ng/mL and limits of detection were less than 4 ng/mL for each drug analyzed. The method demonstrated acceptable recoveries for most of the analytes and good intra- and inter-day precision. Acquisition of data by repetitive full scan GC-MS allows the addition of further analytes to the target menu. 相似文献
12.
Alberto Alemanno 《European Law Journal》2009,15(3):382-400
While most academic attention is currently being paid to the goals and to the merits of the Better Regulation initiative (BR), this article examines the most immediate legal implications stemming from reliance on the main tools of BR (such as systematic impact analysis and consultation procedures on proposed legislation) within the European legal order. Since the BR package boils down into a set of regulatory requirements, enforcement issues are likely to arise. For instance, what if the Commission omits to undertake (or badly performs) an impact assessment of a legislative proposal? Who is currently in charge of ensuring the Commission's compliance with these requirements? After illustrating the existing administrative oversight mechanisms designed to ensure effective compliance with BR requirements, by focusing notably on the recently established Impact Assessment Board, the article examines to what extent the European courts may be called upon to review the respect paid to the requirements of BR by the Commission services. To prevent the BR initiative from turning into a Trojan Horse within its own walls, the Commission is likely to comply with these regulatory requirements, thereby paving the way for the initiative's success. 相似文献
13.
Alberto Chong Gianmarco Len‐Ciliotta Vivian Roza Martín Valdivia Gabriela Vega 《American journal of political science》2019,63(2):323-341
We use a field experiment to evaluate the impact of two informational get‐out‐the‐vote campaigns to boost female electoral participation in rural areas of Paraguay. We find that public rallies had a small and insignificant effect either on registration or voter turnout in the 2013 presidential elections. Households that received door‐to‐door canvassing treatment were 4.6 percentage points more likely to vote. Experimental variation on the intensity of the treatment at the locality level allows us to estimate spillover effects, which are present in localities that are geographically more concentrated, which may favor social interactions and diffusion of information. Reinforcement effects on the already treated population are twice as large as diffusion effects on the untreated. Our results underscore the importance of taking into account urbanization patterns when designing informational campaigns. 相似文献
14.
Kaiji Xie Crupi Antonio Di Minin Alberto Cesaroni Fabrizio 《The Journal of Technology Transfer》2022,47(1):33-62
The Journal of Technology Transfer - This study performs an in-depth analysis of the causes and consequences of team boundary-spanning activities in the context of Chinese technology transfer, by... 相似文献
15.
Enrico Gerace Ph.D. Elena Bovetto M.Sc. Daniele Di Corcia M.Sc. Marco Vincenti M.Sc. Alberto Salomone Ph.D. 《Journal of forensic sciences》2017,62(4):1107-1111
Diphenidine is a dissociative drug that shows several psychotropic effects including euphoria, shifts in perception of reality, hallucinations, and transient anterograde amnesia. In this study, a case of acute intoxication occurring after diphenidine intake is reported. A 30‐year‐old Caucasian male was hospitalized after he was found in a confused and agitated state and unable to communicate. The physical examination displayed tachycardia, miotic pupils, and increased both body temperature and respiratory rate. After a liquid–liquid extraction procedure, GC/MS analysis revealed the presence of diphenidine in plasma and urine at concentrations of 308 and 631 ng/mL, respectively. Methylphenidate and diclazepam were also detected in the plasma. The clinical progress of the patient was favorable, and his symptoms were cured with a symptomatic treatment. The combined circumstantial elements and toxicological results of the case reported revealed the occurrence of an acute intoxication ascribable to the recreational abuse of diphenidine. 相似文献
16.
菲中关系:古老的友谊崭新的气象 总被引:2,自引:0,他引:2
距北京以南330公里的山东省德州市,有一座纪念碑见证着菲中人民之间源远流长的友谊.这座纪念碑是为纪念苏禄王巴杜卡·巴塔拉1417年远行中国而建.这位苏禄王前往北京拜会明朝的永乐皇帝,但在返回途中却不幸因病辞世. 相似文献
17.
Alberto Fuentes-López Aarón Pedreño Sala Elena Romera José Galián 《Science & justice》2021,61(2):150-159
Studying dipterans at the scene of a death can provide essential information for interpreting the evidence and help to reconstruct the events happened to a corpse in the past. Molecular tools have been employed for identification at specific levels in the cases of cryptic species or poorly conserved specimens. Identification of specimens is essential in forensic entomology since each species has a specific growth rate, which determines the calculation of the minimum post mortem interval (minPMI). In addition, phylogeographic reconstruction within a species can help to differentiate the haplotypes from a geographic area, thereby helping to clarify the possible relocation of a corpse. The morphological identification of Sarcophagidae species is often difficult, especially for the females. This is an important Diptera family since some of its species are among the first to reach a corpse, especially in warm areas. In this study, we compared the sarcophagids found in human corpses in forensic cases in Alicante (southeast of Spain) with specimens collected from baited traps in the same area and surrounding provinces. In total, 189 specimens were collected, comprising 72 from forensic cases and 117 from baited traps. Molecular identification was conducted by sequencing the cox1 mitochondrial gene and analyzing the sequences using ABGD, GMYC, and BIN species delimitation methods. The median joining algorithm in the PopART program was used to construct phylogeographic networks. Eight species in the family Sarcophagidae were identified. The most widely collected species were Sarcophaga argyrostoma and Sarcophaga tibialis. The haplotype networks obtained for these species did not indicate a clear geographic distribution of haplotypes. The S. argyrostoma samples from Alcoy were clearly isolated. The results demonstrated that this method is useful for identifying Sarcophagidae samples in forensic investigations and it can be employed for minPMI estimation. 相似文献
18.
Alberto Abadie Alexis Diamond Jens Hainmueller 《American journal of political science》2015,59(2):495-510
In recent years, a widespread consensus has emerged about the necessity of establishing bridges between quantitative and qualitative approaches to empirical research in political science. In this article, we discuss the use of the synthetic control method as a way to bridge the quantitative/qualitative divide in comparative politics. The synthetic control method provides a systematic way to choose comparison units in comparative case studies. This systematization opens the door to precise quantitative inference in small‐sample comparative studies, without precluding the application of qualitative approaches. Borrowing the expression from Sidney Tarrow, the synthetic control method allows researchers to put “qualitative flesh on quantitative bones.” We illustrate the main ideas behind the synthetic control method by estimating the economic impact of the 1990 German reunification on West Germany. 相似文献
19.
Nondestructive Methods for Recovery of Biological Material from Human Teeth for DNA Extraction 下载免费PDF全文
Montserrat Hervella Ph.D. Maitane G. Iñiguez M.Sc. Neskuts Izagirre Ph.D. Alberto Anta Ph.D. Concepción de‐la‐Rúa Ph.D. 《Journal of forensic sciences》2015,60(1):136-141
The extraction of DNA from human skeletal remains applied to forensic, and evolutionary studies do not exclude risks, which are to be evaluated when working with unique specimens that could be damaged or even destroyed. In the present study were evaluated several nondestructive methods for recovering DNA instead of the most currently used pulverization method. Three different procedures to access inside the dental pieces (occlusal perforation, cervical perforation, and cervical cut) have been compared with the aim of recovering as many cell remains as possible to carry out a DNA extraction. Given the DNA quantitation results, a method was proposed that consists of a cervical cut to facilitate the access to the pulp cavity and a subsequent filing of the root canals down to the apex of the dental root. This methodology allows the recovery of both mitochondrial and nuclear DNA, with the minimum deterioration for the dental pieces. 相似文献
20.
Federica Collini M.D. Alberto Amadasi M.D. Alessandra Mazzucchi B.Sc. Davide Porta B.Sc. Valeria Luisa Regazzola B.Sc. Paola Garofalo B.Sc. Annalisa Di Blasio M.D. Cristina Cattaneo Ph.D. 《Journal of forensic sciences》2015,60(5):1290-1294
This study analyses depressed fractures (by blunt force trauma) and circular full‐thickness injuries (drill injuries and gunshot wounds) in charred bones. Fifty bovine ribs (total 104 lesions) were divided into three groups. The first group consisted in 20 depressed hammer‐produced fractures; in the second one, 60 round drill‐holes were produced (30 circular, 30 semicircular); in the third group, 12 fleshed and 12 skeletonized ribs were hit by 9‐mm bullets. Each specimen was carbonized in an electric oven up to 800°C. Morphological and metric analyses were performed before and after: morphological features were preserved, but depressed fractures showed an increase in their dimensions (p‐value < 0.05); the drilled holes shrunk (p‐value < 0.01); the charring cycle increased the number of fractures in samples with gunshot wounds differently in fleshed and defleshed ribs. This study showed the complex behavior of charred bone, for what concerns the interpretation of trauma and how caution should be applied. 相似文献