Homicides in East Berlin from 1980 to 1989. Part I: victimology and phenomenology

At the Institute of Legal Medicine of the Humboldt-University in Berlin 8,580 autopsies were carried out in the period of analysis from 1980 to 1989 - including 139 homicides (= 1.6 %). The first part of this study deals with the victim-related analysis. Furthermore, the criminologically relevant aspects of the phenomenology of crime are described. Apart from the low frequency, the mainly short latency period and the low rate of homicides with removal of the victim are noteworthy.     

Survived crossbow injuries

The Hamburg University Institute of Legal Medicine presents 2 cases of injuries of crossbow arrows where the patients survived. Crossbows are used nowadays as sports and hunting weapons. They are freely obtainable, and since people without practice can shoot them, there are constant injuries and fatal cases. Crossbow arrows have a high penetration force and can even pierce bone. Depending on the tip of the arrow used, they bore or cut through tissue, here damage to the tissue being restricted to the direct surroundings. Due to the elasticity of the tissue, the arrow shaft in the wound track may have the effect of an incomplete tamponade so that major hemorrhaging is prevented. In this condition, the injured person may be conscious and capacitated. From the medical viewpoint, crossbow arrows should therefore be invariably left in the wound, secured against displacement during transport, and only removed in the hospital.     

Image analysis as an adjunct to sodium rhodizonate test in the evaluation of gunshot residues: an experimental study

It is important to investigate the clothing, as well as the body, to determine the range of fire of entry wounds in firearm injuries. Clothing can affect the amount of gunshot residues (GSR) reaching the body and their distribution. The amount and distribution of the GSR also vary according to the distance between the firearm and the target. Sodium rhodizonate test provides valuable data when clothing is available for examination. In the absence of clothing, light microscopic examinations may add additional information regarding the range of fire. In this study, a sodium rhodizonate test was done on 80 garment samples containing the bullet entrance. The 80 calfskin samples were processed histologically and were stained using Alizarin Red S. These were also evaluated with computer-assisted image analysis. Gross residues were seen on military camouflage clothing in samples from < or = 45-cm group. White flannel undershirts under the military camouflage contained rhodizonate-positive particles only around the contact wounds. With image analysis, however, the residues could be detected also in the skin samples of the 2.5-cm- and 5-cm-range groups. We suggest that the image analysis can be combined with other techniques and it can provide valuable data in the determination of entry wounds and also in the estimation of firing distance.     

The problem of single parent/child paternity analysis--practical results involving 336 children and 348 unrelated men

In a certain amount of paternity investigations, only DNA from child and alleged father is analyzed, thus increasing the possibility of false paternity inclusions. The aim of this study was to determine how many wrong paternity inclusions could be detected in a rather small geographical area comparing empirical results from 336 children and 348 men (13-15 STRs were investigated per person). This comparison between each child and all unrelated men (i.e. all putative fathers from the other cases) with an especially designed computer program resulted in 116,004 man/child pairs. Less than three excluding STRs were found in 1666 child/unrelated man pairs (1.44% of the comparisons). At least one unrelated man with only two or less STR mismatches could be determined for 322 children (95.8% of all investigated children). In 26 comparisons no STR mismatches between a child and an unrelated man were detected, thus at least one and up to three "second father(s)" under 350 men could be found for 23 children, if the mother is excluded. Paternity probabilities between 95.475% and 99.996% were calculated. Our results underline the difficulties in motherless paternity cases using only STR analysis and advise great precaution in assigning verbal predicates such as "paternity proven" in those investigations.     

Analysis of the population heterogeneity in Hungary using fifteen forensically informative STR markers

Previous studies have shown that population analyses in Hungary can be of great importance from the viewpoint of the examination of population differentiation. This study provides additional population genetic data of the Hungarian population on the thirteen CODIS core STR loci and on two penta STRs (PentaE, PentaD). Allele frequency and profile databases were generated for four population samples. Comparing the allele frequency values by G-statistic, calculating the FST indices and with the pair-wise comparisons of inter-population molecular variance (AMOVA) the four Hungarian populations could be distinguished.     

A versatile technique for the investigation of gunshot residue patterns on fabrics and other surfaces: m-XRF

With heavy-metal-free ammunitions becoming more and more popular, it is necessary to find methods to visualize patterns of those elements in gunshot residues (GSRs) that are not accessible by chemographic coloring tests. The recently introduced millimeter-X-ray fluorescence analysis (m-XRF) spectrometer Spectro Midex M offers an easy way to record mappings of GSRs containing such elements in order to determine shooting distances as well as the general composition of these particles. A motorized stage enables samples of a maximum size of 20 x 20 cm to be investigated, like fabric, clothes, adhesive tapes (Filmolux films), and polyvinylalcohol gloves of shooter's hands. Human tissues can be measured using a Peltier-cooled specimen holder that is mounted onto the stage. As the spot size of the exiting X-rays lies in the millimeter range, which is adequate for the assessment of the residue patterns for shooting distance determination, a significant reduction in measurement time is achieved compared with mu-XRF methods. Test shots with heavy-metal-free ammunitions were performed on different target materials, like pork skin and fabric, and the elemental distributions of Ti, K, and Ga were determined. In order to show the capability of the spectrometer for conventional lead ammunitions as well, a shot series of 5-100 cm shooting distance and an adhesive tape of a shooter's hand were investigated analogously. A comparison of several methods applied in GSR investigation shows the advantages of the m-XRF method.     

Deaths from drug overdose and toxicity in Turkey: 1997-2001

Turkey is located on the main overland connection between Asia and Europe, making the country potentially vulnerable to drug trafficking and its associated harms. The aim of the present study is to explore the frequency of all deaths from drug overdose and toxicity in Turkey and to describe some of the characteristics of these deaths. We collected data on all deaths from drugs in Turkey between 1997 and 2001 using records from the Council of Forensic Medicine. Data obtained from autopsy reports were retrospectively analyzed. In the present study, 374 deaths from drugs were reported in Turkey, with a mortality rate of 0.17 per 100,000 population. Highest mortality rates were found in Istanbul (0.83) and Gaziantep (0.71). The mean age was 34.0, and most cases (71.7%) were below the age of 40. The proportion of female cases was 13.6%. Opiates were implicated in 91.5% of deaths and benzodiazepines in 25.9%. Two fifths (38.8%) of the cases involved use of more than 1 drug. In 36.6% of cases, the route of final drug administration was by injection. The most common location of death was at a home (33.7%). Interventions to reduce drug use nationally are urgently required. International cooperation in social-educational activities, scientific research, and security measures is essential for this war.     

Comparison of pulmonary autopsy findings of the rats drowned at surface and 50 ft depth

INTRODUCTION: When a body is recovered from the water after a fatal SCUBA diving accident, it is useful to know if the diver was under pressure or not when he/she took his/her last breath, in order to determine the cause and manner of the death. If the victim was under pressure, the air remained in the lungs of the diver will be equal to the environmental pressure. If the body comes to the surface, the air in the lung will expand according to the Boyle's Gas Law and give mechanical damage to the surrounding tissues, due to decreases in environmental pressure. We designed an experimental study to see the difference in pulmonary autopsy findings of the rats that drowned under normobaric and hyperbaric conditions. METHOD: Forty five male, 250-300 g, Sprague Downey adult rats were divided into three groups. Two groups of rats were drowned under normobaric conditions (Groups DS Group DSS) and the third group at 50 ft pressure (Group DD). The pulmonary autopsy findings of the groups were compared. In the light microscopy, the number of the microscopic fields (x10) containing at least one emphysematous area with longitudinal dimension greater than 300 micro m were compared among the groups. RESULTS: The gross examination revealed a prominent swelling of the lungs in all rats in the Group DD, in comparison to that of the Groups DS and DSS. The number of the microscopic fields, which included at least one emphysematous area with longitudinal dimension greater than 300 micro m out of 150 fields from each of the groups DS, DSS and DD, were 88, 101 and 115 respectively. The difference between the group DS and DD was found to be statistically significant. CONCLUSION: We conclude that in investigating the fatal diving accidents, pulmonary autopsy findings give valuable information whether the death occurred at the surface or at the depth.     

Analysis of artificially degraded DNA using STRs and SNPs--results of a collaborative European (EDNAP) exercise

Recently, there has been much debate about what kinds of genetic markers should be implemented as new core loci that constitute national DNA databases. The choices lie between conventional STRs, ranging in size from 100 to 450 bp; mini-STRs, with amplicon sizes less than 200 bp; and single nucleotide polymorphisms (SNPs). There is general agreement by the European DNA Profiling Group (EDNAP) and the European Network of Forensic Science Institutes (ENFSI) that the reason to implement new markers is to increase the chance of amplifying highly degraded DNA rather than to increase the discriminating power of the current techniques. A collaborative study between nine European and US laboratories was organised under the auspices of EDNAP. Each laboratory was supplied with a SNP multiplex kit (Foren-SNPs) provided by the Forensic Science Service, two mini-STR kits provided by the National Institute of Standards and Technology (NIST) and a set of degraded DNA stains (blood and saliva). Laboratories tested all three multiplex kits, along with their own existing DNA profiling technique, on the same sets of degraded samples. Results were collated and analysed and, in general, mini-STR systems were shown to be the most effective. Accordingly, the EDNAP and ENFSI working groups have recommended that existing STR loci are reengineered to provide smaller amplicons, and the adoption of three new European core loci has been agreed.