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991.
D17Z1探针点杂交DNA定量研究   总被引:1,自引:1,他引:0  
本研究化学合成了高等灵长类特异性α卫星寡核苷酸片段(D17Z1),经辣根过氧化物酶标记、分子杂交、化学发光法检测,建立了高等灵长类特异性DNA精确定量的方法.制备了DNA浓度梯度标准对照.对人类DNA,猴、猪、牛、羊、鸡、兔、鱼、小鼠等常见动物DNA及JMl09大肠杆菌、入DNA、φ174DNA等微生物DNA进行了定量分析.结果表明,应用该方法对人类DNA定量不受非高等灵长类动物DNA与微生物DNA的影响,可实现组分定量;灵敏度测试,可对0.12ng的人类DNA进行定量,适用于法医DNA检验定量分析.  相似文献   
992.
More than 60 years after an illicit love affair had occurred between Erika H, wife of a Wehrmacht soldier, and a Polish slave worker during World War II, we could clarify the blood relationships of her daughter Uta. When Erika H had become pregnant both of the men could have fathered the child. Erika H was found guilty of fraternization and imprisoned at Ravensbrück concentration camp. She gave birth to Uta and died there in 1944. Uta survived the war as did Erika's husband Gustav, who accepted Uta as his child. Blood samples from family members were taken and DNA extracted. A panel of 16 short tandem repeat (STR) loci were amplified and separated by capillary electrophoresis and the likelihoods calculated using the MLINK software. The combined genotypes yielded a cumulative likelihood ratio of over 200,000 against paternity of Gustav H. This case serves to illustrate the utility of STR profiles for complex deficiency kinship analysis.  相似文献   
993.
Forensic taphonomy in the marine context recently received growing attention. However, only limited information is available about the fate of human bodies at greater sea depth. Following two fatal aircraft accidents (west of Namibia, south of Sicily) human remains were recovered from a depth of 540-580 m (both cases) after 3 months (Namibia)/34 days (Sicily). In the Namibia case fully skeletonized bones were lifted. In the Sicily case a complete, dressed body was found exhibiting a partially skeletonized skull, starting adipocere formation and pink teeth. The rate and mode of decomposition of human bodies in the deep sea varies considerably and is mainly influenced by the local faunal composition. Of special relevance for the understanding of both cases was the oceanographic observation that the highly efficient necrophageous lyssianassids are abundant off Namibia but are rare in the Mediterranean, emphasizing the importance of collaboration of forensic and marine scientists in such case work.  相似文献   
994.
The aim of this study was to reveal histopathological features for differential diagnosis of skin lesions caused by electrocution, flames and abrasions. Based on the causes of the lesions, cases were assigned into three groups. Group 1 included 30 deaths from electric shock. Group 2 included 30 individuals with flame burns who died in the fires. Group 3 included 30 deaths from traffic accidents, from which the individuals had abrasions. Data from the crime scene investigations and macroscopic and microscopic findings from the autopsies allowed determination of the cause of death in all cases. The features of the lesions examined under the light microscope were intraepidermal separation, subepidermal (dermoepidermal) separation, coagulation necrosis in the epidermis, nuclear elongation in the epidermis, dark-staining epidermal nucleus, depth of homogenization in the dermis, and nuclear elongation in the epithelium of hair follicles. A significantly high rate of electrical lesions had intraepidermal separation. The rate of subepidermal separation was slightly more significant in flame burns. A significantly higher rate of electrical lesions had both intraepidermal and subepidermal separation. The rate of coagulation necrosis in the epidermis was significantly the highest in electrical lesions. Although the severity of nuclear elongation was the most significant in electrical lesions, varying degrees of nuclear elongation in the epidermis were present in all three groups. Dark staining of the epidermal nuclei was present in all lesions except for one electrical lesion, though the severity of staining was significant in the abrasion group. The depth of homogenization was slightly more significant in the abrasion group. The rate of nuclear elongation in the epithelium of the hair follicles was significantly lower in the abrasion group. The results of this study revealed that certain morphological changes determined under a light microscope could help the differential diagnoses of electrical lesions, flame burns and abrasions.  相似文献   
995.
Shooting range injuries are generally caused by ballistic accidents, and so far no burn disaster has been reported. In this article we reported a disaster caused by a gunpowder explosion in an indoor shooting range area in Istanbul, Turkey. Fourteen injured people were evacuated from the scene. Our burn center accepted 7 of them. Of the 7 injured people, 2 who were accepted by our burn center, and 3 people who were admitted by another center died. It is clearly identified how this mechanism of injury differs from that of usual burn injuries, due to both the high temperature generated, and the combination of hot and toxic gases produced by the explosion. We described the features of burn injury, and possible reasons of burn disasters.  相似文献   
996.
997.
When rapid economic growth catapults a country within a few years from the margins of the global economy to middle power status, does global regulatory governance need to brace for a challenge to the status quo? To answer this question, we extend the power transition theory of global economic governance to middle powers: A rising middle power should be expected to challenge the international regulatory status quo only if increasing issue-specific strength of its regulatory state coincides with preferences that diverge from the preferences of the established powers, which are enshrined in the status quo. We examine this argument empirically, focusing on South Korea in the realm of competition law and policy. We find that South Korea, a non-participant in the international competition regime until the 1980s, developed in the 1990s substantial regulatory capacity and capability and thus “spoiler potential.” At the same time, however, its policy preferences converged upon the norms and practices established by the United States and the European Union, albeit with some distinct elements. Under these conditions, we expect a transition from rule-taker to rule-promoter. We find that South Korea has indeed in recent years begun to actively promote well-established competition law and policy norms and practices – supplemented by its distinct elements – through technical assistance programs, as well as various bilateral channels and multilateral institutions. The findings suggest that the power transition theory of global economic governance is usefully applicable to middle powers, too.  相似文献   
998.
Journal of Experimental Criminology - To test whether normative and non-normative forms of obligation to obey the police are empirically distinct and to assess whether they exhibit different...  相似文献   
999.
1000.
A stationary eddy that constantly re-forms in the riverbed of the evolution of Western normative institutions, Legal Critique dates back, beyond modernity, to the beginning of the so-called Common Era. But critique also shapes the historical review of earlier phases of this evolution, and this not only as a method of the examination of sources, but also as a transferential displacement that tends to project into history the divides and aporias which define a present political situation. Unsurprisingly, this proceeding betrays more about current conceptions than it reveals about those of the past. The fate of the philosophical topic of immanence and transcendence and that of the proto-modern politics inaugurated by the distinction of God’s absolute versus ordered power offer a significant case in point. Certain critical orientations find in the long and complex history of these divides merely their own anticipated echo. Yet, the split between the adepts of an Aristotelian universe rooted in the being of the good and the followers of Spinoza, accustomed to absolute power and immanent causality, resists such simplifications and warrants a new examination.  相似文献   
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