Aqueous phase hexylchloroformate derivatization and solid phase microextraction: determination of benzoylecgonine in urine by gas chromatography-quadrupole ion trap mass spectrometry.

A derivatization/solid phase microextraction (SPME) method for the determination of benzoylecgonine in urine was developed. The derivatization is conducted directly in 1 mL of urine while sonicating for 3 min with 12 microL of hexyl chloroformate and 70 microL of a mixture containing acetonitrile:water:hexanol:2-dimethylaminopyridine (5:2:2:1 v/v), yielding benzoylecgonine hexyl ester (BHE) as the product. After the 3 min period, an aliquot of 250 microL is transferred to a vial for SPME. After the desired extraction time the 100 microns polydimethylsiloxane SPME fiber was transferred to the GC-MS for separation and analysis with a quadrupole ion trap mass spectrometer. The hexyl chloroformate derivatization and SPME procedures were optimized for compatibility and sensitivity. The method was found linear for 0.10 to 20.0 micrograms/mL (r2 = 0.999) of benzoylecgonine in urine using benzoylecgonine-d3 as an internal standard (1.5 micrograms/mL). Intra-day precisions were 8.8 and 6.8% RSD for 0.30 microgram/mL and 17 micrograms/mL benzoylecgonine standards in urine (n = 6), respectively. Inter-day precision (n = 3) were < or = 3.3% RSD, indicating good reproducibility. A detection limit of 0.03 microgram/mL (S/N = 3) was achieved, thus making the SPME method a simplified alternative to SPE for GC-MS confirmation after EMIT tests for benzoylecgonine which have a cutoff of 0.30 microgram/mL. Quantitative results by SPME and SPE of two clinical urine specimens known positive for cocaine by EMIT were in excellent agreement. Benzoylecgonine was detected by the derivatization/SPME method in 22 out of 22 other urine specimens known positive for cocaine.     

Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae) established in the vicinity of Knoxville, Tennessee, USA

The hairy maggot blow fly, Chrsomya rufifacies (Macquart) (Diptera: Calliphoridae) was collected in large numbers as both adults and immatures in the Knoxville, Tennessee, area during 1998 and is likely established there. The distribution of this species in the Old World, isothermal data, and its collection from mid-Michigan during 1998 suggest that it will eventually occupy most of the U.S. The forensic importance of C. rufifacies makes it probable that it will factor into an increasing number of medicolegal cases, but the expanding distribution of this species decreases its utility as a geographic indicator when postmortem movement of decedents is suspected.     

Medical malpractice problem.

The medical malpractice problem is extremely complex and is perceived very differently by health care providers, patients, and other segments of society. As a widely recognized problem, it is of relatively recent origin. Its potential societal consequences include disruption of health services, waste and maldistribution of economic and human resources, and a severe strain on a variety of our traditional social institutions. The "crisis" of malpractice is a product of many forces, including a disturbing level of negligent and improper medical care, frequently unrealistic patient expectations, and the growing "philosophy of entitlement" which is rampant among Americans. Responses to the problem have sometimes been ill-advised and emotional. Most often, the problem is seen as one of inadequate or overpriced malpractice insurance, though careful analysis suggests that insurance issues are merely symptoms of the real problem. Nevertheless, insurance-based solutions have proven to be politically expedient and have produced at least temporary alleviation of the problem. Ultimately, though, other approaches will be necessary, and careful, objective research is required to identify and test long-term options.     

Moving ahead: Five essential elements for working effectively with girls

Quite a bit of literature these days addresses what is believed to be an increase in the frequency and severity of girls' problem behaviors and the need for gender-responsive treatment. The extent to which this literature has been translated into juvenile justice practices, however, appears limited by several factors. This article briefly discusses these impediments, and offers suggestions for five essential elements that reflect the current state of knowledge regarding effective intervention with girls involved in the juvenile justice system.     

The Impact of Job Characteristics on Private Prison Staff: Why Management Should Care

The number of private prisons run by corporate security businesses has increased rapidly throughout the past two decades. There has been a parallel increase in literature, both pro and con, comparing the efficiency and effectiveness of private and public prisons; however, private prison staff has been largely ignored. OLS regression analysis of a survey of 160 employees at a Midwestern private prison facility showed that the job characteristics of job stress, supervision, and job variety were far more important than personal characteristics of race/ethnicity, gender, age, tenure, education and position in influencing staff job satisfaction and organizational commitment. Specifically, job stress had the largest impact on job satisfaction, while quality of supervision had the greatest impact on organizational commitment.