Phosphoglucomutase1 and 6-phosphogluconate dehydrogenase types in human skin and adipose tissue

Attempts were made to detect phenotypes of the enzymes phosphoglucomutase (PGM) and phosphogluconate dehydrogenase (PGD) in human skin and adipose tissues. Both enzymes could be typed using approximately 3 mg wet weight of tissue. Phenotypes could be distinguished after up to 15 days of aging for PGM and ten days of aging for PGD. Analysis of isoenzymes is potentially useful for mediolegal identification of human skin and adipose tissue.     

Double replica electroblotting for the simultaneous phenotyping of C6 and C7, and its application to the examination of bloodstains

A new reliable and reproducible technique for the simultaneous determination of C6 and C7 types is presented, which employs double replica electroblotting after isoelectric focusing. It permitted clear discrimination of both C6 and C7 components, and the patterns were nearly comparable to those demonstrated separately. The population data obtained by this new technique fitted the genetic hypothesis. The present double replica electroblotting method was successfully applied to the combined phenotyping of C6 and C7 from bloodstains which were stored at room temperature for up to 4 weeks. The method is quite suitable for medicolegal examination of bloodstains particularly for the saving in the amount of sample.     

Deception and Fear in Politically Oppressive Contexts: Its Trickle-Down Effect on Families

The mystified reality, restricted options, and inherent risks of living in countries under a repressive political regime trigger survival tactics that reduce individual's reliance on social support, as well as semantic and cognitive restrictions and alternative codes, and silences that translate into symptoms. While this is the case for the average citizen, it is even more pronounced in individuals and families directly touched by the repressive apparatus. These processes are discussed and two clinical examples are provided to illustrate them.     

Direct fluorometric determination of cyanide in human materials

A detailed procedure of a new simple fluorometric assay for cyanide in human blood, stomach contents and urine is presented. This method requires neither diffusion nor distillation of cyanide from samples; small amounts of the samples were directly added to incubation mixtures, which were then heated at 80 °C for 45 min and acidified for fluorescence measurements. Our method is much more rapid than the previous ones. Some data are presented on specificity and sensitivity of the present method.     

Polymorphism of EsD by isoelectric focusing: description of the new allele EsD*Kofu and phenotyping in bloodstains

The polymorphism of EsD was investigated in 1115 unrelated Japanese individuals by isoelectric focusing. Besides the three common phenotypes two heterozygotes EsD 7-1 and EsD 7-2 were observed. The gene frequencies were: EsD*1 = 0.6234, EsD*2 = 0.3663, and EsD*7 = 0.0103. In addition, a rare variant was detected in a probandus living in the city of Kofu. The family analysis suggested the hereditary occurrence of a new allele EsD*Kofu. The isoelectric focusing method was successfully applied to phenotyping EsD in bloodstains; each phenotype was demonstrated at 37 degrees C for up to 2 weeks, at room temperature for up to 9 weeks, and at 4 degrees C for over 20 weeks after stain formation.     

A stability study on Gc subtyping in bloodstains: comparison by two different techniques

The limits of determination of Gc subtypes in bloodstains were compared between the immunofixation method and the sulfosalicylic acid precipitation method using isoelectric focusing on polyacrylamide gel. By the immunofixation method Gc subtyping in bloodstains was successfully made at 37 degrees C after 7 weeks, at room temperature after 17 weeks and at 4 degrees C even after 25 weeks storage. By the sulfosalicylic method Gc subtypes were no longer able to be determined a few weeks after stain formation. The superiority of the results obtained by the immunofixation method makes it the recommended method for the Gc subtyping from bloodstains in medicolegal practice.     

A practical method for the accurate determination of methemoglobin in blood containing carboxyhemoglobin

Kinetics of the oxidation of carboxyhemoglobin (HbCO) by potassium ferricyanide was studied photometrically in a weakly acid solution. An increase in the absorbance at 630 nm reached a maximum within 10 min when over a 100-fold excess of ferricyanide to hemoglobin iron was used. A slight decrease in the absorbance was observed after completion of the reaction when over a 500-fold excess of the reagent was used. In the presence of 0.4% Sterox SE, the absorbance began to decrease without complete oxidation. From these findings, a simple, rapid and accurate method for the determination of methemoglobin (Met-Hb) in blood was devised. The method was compared with two other methods, using 11 blood samples containing various amounts of HbCO, and proved to be suitable for blood containing elevated HbCO as well as for ordinary blood.     

Political protection and the origins of the Gulf Cartel

The history of drug trafficking in Mexico appears to be strongly influenced by specific features of the post-revolutionary authoritarian regime that has been characterized by its rampant corruption and poor levels of accountability. This is a rigorous empirical case study of state domination of the relationship with traffickers in a particular historical epoch and place. Based on research conducted through the examination of historic sources, this work explores the hypothesis that some political figures might have been colluding with members of criminal organizations, with the aim of protecting their businesses and fostering their consolidation. Such collusion has hindered the institutional functioning of key law enforcement and judicial institutions in the country. The present analysis considers these relationships in the course of three decades, the 1960s through the 1990s, and focuses on the Mexican state of Tamaulipas, where one of the most powerful and dangerous organized crime groups emerged, the so-called Gulf Cartel.     

Practical developments in law science and policy: efforts to protect the traditional group knowledge and practices of the Shuar, an indigenous people of the Ecuadorian Amazon

The purpose of this article is to broadly outline a problem in the context of relatively recent activity in the Amazon Basin. My colleagues and I found the Policy Sciences framework to be a useful blueprint for comprehensive issue analysis. The five intellectual tasks of the policy-oriented approach to solving problems helped us to clarify the apparent goals of the primary actors involved; identify certain trends associated with the problem, including estimations of their magnitude and implications; understand several conditioning factors which could impact (or have already had an impact on) the achievement of the goals identified; recognize several projections anticipated from a normative standpoint in light of the trends examined; and make some observations, including possible strategies and their alternatives, which might enable the Shuar to maximize benefits and minimize costs.