STR Profiles from DNA Samples with "Undetected" or Low Quantifiler™ Results

Abstract:  Screening methods capable of identifying DNA samples that will not yield short tandem repeat (STR) profiles are desired. In the past, quantitation methods have not been sensitive enough for this purpose. In this study, low level DNA samples were used to assess whether Quantifiler™ has a minimum quantitation value below which STR profiles would consistently fail to be detected. Buccal swabs were obtained and the DNA extracted, quantified, and serially diluted to concentrations ranging from 0.002 to 0.250 ng/μL. Samples were analyzed once with Quantifiler™, followed by Profiler Plus™ amplification and capillary electrophoresis analysis. An absolute minimum value below which STR results were unobtainable could not be defined. From the 96 low level samples tested, STR loci (including one full profile) were successfully amplified and detected from 27% of the samples "undetected" by Quantifiler™. However, no STR alleles were detected in 73% of these "undetected" samples, indicating that Quantifiler™ data may be useful for predicting STR typing success.     

Land tenure regimes and state structure in rural Africa: implications for forms of resistance to large-scale land acquisitions by outsiders

This paper argues for seeing African land tenure regimes as institutional configurations that have been defined and redefined as part of state-building projects. Land regimes have built state authority in the rural areas, fixed populations in rural territories, and organised rural society into political collectivities subject to central control. Land tenure regimes can be understood as varying across subnational jurisdictions (rather than as invariant across space) in ways that can be grasped in terms of a conceptual distinction between neo-customary and statist forms (rather than as infinitely diverse). Differences between the two have implications for the character of political authority in the rural areas, the nature of political identities and community structure, and the nature of property and land claims. These political effects are visible in differences in the forms of local protest and resistance to commercial land acquisitions in peri-urban Kumasi, Ghana, where a neo-customary land regime prevails, and the Kiru Valley of northern Tanzania, where land institutions are decidedly statist.     

Gender Determination of Adult Individuals by Three‐Dimensional Modeling of Canines

Gender determination is a fundamental issue in forensic anthropology. Many techniques based on bone and dental remains have been proposed. It is not always possible to implement the techniques using bones, but teeth are often perfectly preserved. It has been demonstrated that the canine has the greatest sexual dimorphism, and the aim of this work was to provide an easy and accurate dental technique for determining the gender in the absence of other skeletal elements. The sample was composed of 210 CT scans with four healthy canines. The 840 canines were modeled using MIMICS^® 10.01 software. The total volume of each tooth was determined. Seven mathematical models were determined by binary logistic regressions and ranked in order of relative performance. The seven proposed predictive models thus performed (0.910 ≤ AUC ≤ 0.938), with overall rates of correct predictions between 82.38 and 85.24%. The 4‐canine model is the most powerful for predicting the gender.     

Agenda Setting in Emergent R&D Policy Subsystems: Examining Discourse Effects of the 21st Century Nanotechnology Research and Development Act

The literature on government responsiveness to societal issues is extensive but provides a mixed assessment of effectiveness. We examine this issue in the case of policy addressing effective and safe management of research and development in the emerging field of nanotechnology. Specifically, we examine the agenda setting effects of the 21st Century Nanotechnology Research and Development Act (the Act), a piece of legislation designed to be implemented by a network of actors in the nanotechnology research and development policy subsystem. We adopt a public values lens in our examination of discourse related to societal concerns. Policy documents from Congress, an agency, and federal funding recipients are examined. Findings suggest a narrowing of public values discourse around more specific societal concerns in the documents crafted after the Act was passed.     

Participation in Micro-Finance Programmes and Women’s Mental Health in South Asia: A Modified Systematic Review

Micro-finance provides financial services to poor women from developing countries where cultural and social constraints limit their opportunities for economic advancement. Using Forbes’s process of conducting systematic review, 12 quantitative studies from South Asia reporting on the impact of micro-finance on women’s mental health outcomes were analysed. Overall, studies revealed that the duration and depth of involvement in micro-finance activities would make a difference in women’s mental health and not just receiving loans; however, rigorous programme evaluation is needed. Collaborative actions by micro-finance and health care practitioners to recognise women’s mental health needs and to strengthen measures to optimise the beneficial effects of micro-finance must be considered.