DNA数据库建设中批量样本直接扩增检验法的应用

目的研究批量样本直接扩增法在DNA数据库建设中的应用。方法打孔机打取血滤纸600份,剪取芝麻大小的口腔拭子300份,放入96孔扩增板,加入扩增试剂后直接扩增,并对其中200份血滤纸用磁珠法,100份口腔拭子用96孔板Chelex-100法,经DNA提取后扩增检验进行比较。结果血滤纸采用直接扩增法及磁珠法STR检测成功率均为100%;口腔拭子采用直接扩增法的成功率为95%,采用96孔板Chelex-100法的成功率为94%。结论血滤纸和口腔拭子通过直接扩增均能获得很好的DNA分型结果,该方法省时省力,可用于DNA数据库建设。     

Safeguard Measures under the China-Australia FTA: The WTO-Compatibility Perspective

Since the Sino-Australia Free Trade Agreement came into force, the possibility of applying safeguard measures is also increasing along with the rapid growth of their bilateral trade. There are different approaches of applying bilateral and global safeguard measures in the FTAs, in addition to the WTO-compatibility requirements, under the multilateral trading system. GATT Article 24 neither entitles nor forbids applying the bilateral safeguard measures in the FTA context straightforwardly. However, requirements by the Safeguards Agreement as well as the ‘parallelism principle’ as established in dispute settlements must be complied with when a party excluding its FTA partner from its application of global safeguards, otherwise its WTO-compatibility will be challenged.     

论警察职业道德教育的三重维度——基于人民警察核心价值观的视角

人民警察核心价值观是正确认识和评价警察职业道德现状的基本尺度,为我们再次审视警察职业道德教育提供了新的视角。以人民警察核心价值观为指导加快警察职业道德教育的发展具有理论和现实的双重意义。其中,以人为本是动力维度,促进人的全面发展是价值维度,理论联系实际是发展维度。     

邻避冲突对国家安全的影响及对策

邻避冲突是指由邻避设施所引发的,居民有组织地通过正式或者非正式的手段向党政机关或者运营商表达意愿、提出要求,对国家和社会造成一定影响的行为或者活动。目前,我国邻避冲突呈现出发生频繁化、对象广泛化、过程组织化以及目的政治化等特点。邻避冲突不仅影响了社会稳定和国家经济安全,更对国家意识形态安全和政权安全造成了危害,必须从建立健全协商机制、完善风险消减机制、加强舆情回应和监控机制、依法治理邻避冲突事件等方面入手提出对策。     

公安机关政务微媒体舆情应对策略研究

近年来涉警类社会热点事件频发,一旦引导处置不当极易引发网络舆情危机。当前,公安机关政务微媒体在舆情应对中存在着应对意识滞后,引导资源分散,应对方法陈旧,运行维护不规范等问题。针对此类问题,公安机关政务微媒体在舆情应对过程中,应优化应对理念,健全应对处置机制,提升应对管控能力,完善应对激励考核机制,只有这样,才能在完善公安机关政务微媒体运行机制,提升涉警舆情应对能力,发挥政务微媒体自身价值及构建和谐警民关系方面得到助力和有所作为。     

通过辨别、对比和检验材料中的变化测定文件日期

对一份文件最早制成时间的检验一直以来都是全世界文检界的难题之一。核实可疑文件的制成时间没有统一的方法,目前主要通过对纸张、墨迹、打字机、复印机、打印机、传真机、印刷品,以及笔迹和签名等因素的检验来确定一份文件的制成时间。文件检验人员需要充分利用他们手中的文件的内容及文件周边信息所反映出来的条件和材料,进而得出结论。     

行政诉讼行政首长出庭应诉制度:司法政治学的视角

新《行政诉讼法》修订颁布之后,行政首长出庭应诉制度正式成为一项带有鲜明中国特色的司法运作机制。从制度变迁的角度来看,行政首长出庭应诉制度经历了一个从地方政策试验到中央立法吸纳的过程。从各地关于行政首长出庭应诉制度的政策文本出发,不难发现社会稳定压力与地方法制竞争的外部因素影响,而且行政首长出庭应诉制度的实践运作,也主要依赖于数字考核的指标控制而非行政法治的自我拘束。从中国行政诉讼模式变迁的大背景下观察,行政首长出庭应诉与行政诉讼协调和解、行政诉讼司法建议制度根源自相同的法理,均旨在实现纠纷的实质性化解,而不再将合法性判断视为行政诉讼的核心议题。     

Genetic Variants in KCNE1, KCNQ1, and NOS1AP in Sudden Unexplained Death During Daily Activities in Chinese Han Population

Fifty‐six sudden unexplained death (SUD) cases were collected from Chinese Han population, which occurred during daily activities and were autopsy negative in comprehensive postmortem autopsy. The coding exons of potassium channel genes KCNE1, KCNQ1, and nitric oxide synthase gene NOS1AP were sequenced. A synonymous mutation, KCNE1 F54F T>C was identified in 2 SUD cases, which was absent in the control subjects. Neither genotype nor allele frequencies of KCNE1 and KCNQ1 exhibited a significant difference between the SUD and control group. In contrast, the allele frequency (p = 2.7 × 10⁻¹⁰) and genotype frequency (p = 5.9 × 10⁻⁷) of rs3751284, and the genotype frequency (p = 2.9 × 10⁻²) of rs348624 in NOS1AP of SUD were significantly different from that of controls (p < 0.05). Our study suggested that rs3751284 and rs348624 might be susceptibility loci for SUD during daily activities. Larger sample sizes and further molecular studies are needed to confirm or exclude an effect of the NOS1AP SNPs on SUD risk.