Bericht über die 32. Fachtagung der Gesellschaft für Umweltrecht

Ohne Zusammenfassung     

Coping with Canadian federalism: the case of the Federation of Canadian Municipalities

Abstract: Since the early 1960s, the only pan‐Canadian association of municipalities has shown two decades of decline followed by two decades of growth. This paper analyses this decline and subsequent growth in the context of the changing position of municipalities with Canada, particularly those in Quebec. Examination of the association's recent history provides useful insights into how Canada's municipalities cope with Canadian federalism. It provides lessons as to how best to prosper as a pan‐Canadian organization concerned with what are mainly matters of provincial interest. The paper's theoretical contribution includes reference to the nature and roles of interest groups in Canadian federalism and to the application of structural contingency theory. Sommaire: Depuis le début des annees 1960, l'unique association pancanadienne de municipalités a connu deux décennies de déclin suivies par deux décennies de crois‐sance. Le présent article analyse ce déclin et cette croissance dans le contexte de la position changeante des municipalites à l'égard du Canada, en particulier celles du Québec. L'examen de la récente histoire dee l'association suscite des réflexions utiles sur la maniére dont les municipalités au Canada font face au fédéralisme canadien. II explique comment un organisme pancanadien principalement préoccupé par des questions d'intérséCt provincial pour prospérer le mieux possible. La contribution théorique de l'article fait référence à la nature et aux rôles des groupes d'intéráCts dans le fédéralisme canadien et à l'application de la théorie de contingence structurale.     

Pseudo-Stab Wounds: Putrefactive Dehiscence of Remote Surgical Incisions Masquerading as Stab Wounds

Abstract:  Artifacts due to decomposition can be mistaken for antemortem injury leading to an incorrect suspicion of foul play. We describe an instance of postmortem wound dehiscence that mimicked antemortem stab wounds. A man with a history of colon cancer and substance abuse was found dead. There were advanced putrefactive changes and multiple defects of the anterior torso that resembled stab wounds. Subsequent investigation revealed that 11 months earlier, he had a laparoscopic-assisted colon resection that involved surgical incisions corresponding in location and size to the above defects. Putrefactive gases may cause bloating of the body. This distension may cause recent and remote healed incisions to dehisce. Correlation of these "defects" at autopsy with the antemortem clinical history is crucial in determining their etiology.     

Determination of ibogaine and noribogaine in biological fluids and hair by LC-MS/MS after Tabernanthe iboga abuse Iboga alkaloids distribution in a drowning death case

Tabernanthe iboga belongs to the Apocynaceae family. In this study, we report the case of a 37-year-old black male working as a security agent in Paris and found dead naked on the beach in Gabon after consumption of iboga. Autopsy revealed a drowning fatality and a myocardial abnormality (myocardial bridging). Samples of blood, urine, bile, gastric content, liver, lungs, vitreous, spleen and hair were taken. Biological fluids were liquid-liquid extracted with saturated NH4Cl pH 9.5 and methylene chloride/isopropanol (95/5, v/v) in presence of clonazepam-d(4), used as internal standard. After decontamination with dichloromethane, hair was cut into small pieces then sonicated for 2h in saturated NH4Cl pH 9.5 before extraction by methylene chloride/isopropanol (95/5, v/v). After evaporation the residues were reconstituted in methanol/ACN/formate buffer pH 3, from which 10 microL were injected into an ODB Uptisphere C(18) column (150 mm x 2.1mm, 5 microm) and eluted with a gradient of acetonitrile and formate buffer delivered at a flow rate of 200 microL/min. A Quantum Ultra triple-quadrupole mass spectrometer was used for analyses. Ionization was achieved using electrospray in the positive ionization mode (ESI). For each compound, detection was related to three daughter ions (ibogaine: m/z 311.4-->122.1, 174.1 and 188.1; noribogaine: m/z 297.4-->122.1, 159.1 and 160.1; clonazepam-d(4): m/z 319.9-->218.1, 245.1 and 274.1). Ibogaine and noribogaine were detected in all autopsy samples. Hair segmentation was not possible as hair was very short and frizzy. Concentrations of 1.2 and 2.5 ng/mg, respectively were detected. Neither other licit or illicit drugs nor alcohol were found. The presence of ibogaine and noribogaine in all autopsy samples was consistent with the recent absorption of Tabernanthe iboga, which was assumed to be responsible of the drowning fatality. The history of exposure, regarding hair analysis, is discussed. LC-MS/MS appears to be the best method for analyzing complex and poorly volatile alkaloids in autopsy samples and particularly in hair, due to the presence of a nitrogen ring and the relatively low concentrations to be measured.     

Rapid STR analysis of single source DNA samples in 2 h

The establishment of offender DNA databases is critical to future crime prevention. Many countries have established databases or are in the process of passing database legislation. With new legislation the number of samples that will be collected could begin to exceed the testing capacity of many labs leading to backlogs.Two bottlenecks in the workflow that can contribute to a backlog of samples are DNA purification and PCR cycling time. The average purification time is approximately 2 h and the average cycling time of current STR kits is approximately 3 h. To address the second problem we investigated alternative DNA enzymes to decrease PCR cycling time. It was necessary to balance the increase in time to result against the need to address factors which can impact interpretation of a DNA profile such as: generation of stutter products, non-template addition, intra-locus balance, accuracy, and species specificity.Initial feasibility studies demonstrate that alternative enzymes can decrease PCR cycling time. The data show that this assay can increase throughput, providing results in less than 2 h. However, decreasing PCR cycling time will have an affect on multiplex STR performance.     

Vitreous Fluid and/or Urine Glucose Concentrations in 1335 Civil Aviation Accident Pilot Fatalities*

Abstract: During aviation accident investigations, vitreous fluid and urine samples from pilot fatalities are analyzed for glucose and blood for hemoglobin A_1c (HbA_1c) to monitor diabetic pilots and to discover other pilots with undiagnosed/unreported diabetes. The prevalence of elevated glucose concentrations in fatally injured pilots was evaluated by searching the Civil Aerospace Medical Institute’s Toxicology Database for the period 1998–2005. Out of 1335 pilots involving 363 vitreous fluid, 365 urine, and 607 vitreous fluid and urine analyses, 43 pilots had elevated glucose in vitreous fluid (>125 mg/dL) and/or in urine (>100 mg/dL). Of the 20 pilots whose blood samples were analyzed, nine had >6% HbA_1c—four were known diabetics, and five were unknown diabetics. Urinary glucose levels were elevated in all 13 known hyperglycemic pilots. A considerable number of pilots (30 of 43) had elevated glucose and HbA_1c (5 of 20), suggesting undiagnosed/unreported diabetic conditions.     

Development of a Nomenclature System for a Canine STR Multiplex Reagent Kit*†

Abstract: Despite the popularity of dogs in US households, canine DNA evidence remains largely untapped in forensic investigations partially because of the absence of well‐defined forensic short tandem repeats (STRs), lack of standardized and validated PCR protocols, STR reagent kits, and poorly developed nomenclature. A nomenclature system was established based on internationally recognized recommendations for human forensic STRs for a recently developed canine STR reagent kit. Representative alleles were sequenced from each of the 18 STRs and the sex‐typing marker included in the kit. This study also reflects on the impact of point mutations, insertions, and deletions within and outside the STR core repeat structures. An understanding of the STRs’ sequence and repeat structures will enable development of a robust and reliable allele nomenclature and improve the accuracy and precision of allele fragment sizing in canine forensic profiling. The expected allele sizes have been calculated, and their repeat stuctures defined based on sequence information.