Viability of Human Articular Chondrocytes Harvested Postmortem: Changes with Time and Temperature of In Vitro Culture Conditions

Different studies of long‐term chondrocytes viability have shown a gradual reduction as a function of time and ambient temperature. The aim of our in vitro study was to establish chondrocyte postmortem viability curves for 4°C, 11°C, 23°C, 35°C during 63 days after the donors' death. Osteochondral cylinders were procured from the knees of 16 male donors (20–47 years), stored in preservation media that was not changed, and analyzed in 3‐day intervals using a confocal laser scanning microscope. A significant influence of time on viability was found from Day 9 (p = 0.0029) and onwards (p < 0.0001). The lowest overall chondrocyte viability was at 35°C, followed by 4°C (p < 0.0001). The conditions used in this in vitro analysis suggest that similar viabilities may occur while in situ in the decedent. Further studies of chondrocyte viability from individuals with known postmortem intervals may show premise to help evaluate time since death in the late postmortem interval.     

The Optimal Combination of Cartilage Source and Apparatus for Long‐Term In Vitro Chondrocyte Viability Analysis

Abstract: Most studies of long‐term chondrocytes survival were for tissue banks. They showed a gradual reduction in the viable chondrocytes percentage as a function of time and ambient temperature, but the samples were harvested under optimal conditions. The aim of our study was to determine the most reliable combination of cartilage source and assay for the in vitro postmortem chondrocyte viability analysis in the conditions that imitate a dead body. Osteochondral cylinders were procured from femoral condyles and talar trochleas of three male donors and stored in the cell culture media at 4 ± 2°C and 23 ± 2°C. The samples were analyzed by a cell viability analyzer and a confocal laser scanning microscope (CLSM) initially 24–36 h after death and then in 4‐week intervals. The results reconfirmed the significant influence of time (p = 0.0002), but not of the temperature (p = 0.237). The largest reproducibility was presented for the knee joint and the CLSM.     

Suicidal single gunshot injury to the head: differences in site of entrance wound and direction of the bullet path between right- and left-handed--an autopsy study

The aim of this study was to determine the differences in the anatomical site of a gunshot entrance wound and the direction of the bullet path between right- and left-handed subjects who committed a suicide by a single gunshot injury to the head. The retrospective autopsy study was performed for a 10-year period, and it included selected cases of single suicidal gunshot head injury, committed by handguns. We considered only contact or near-contact wounds. The sample included 479 deceased, with average age 47.1 ± 19.1 years (range, 12-89 years): 432 males and 47 females, with 317 right-handed, 25 left-handed, and 137 subjects with unknown dominant hand. In our observed sample, most cases involved the right temple as the site of entrance gunshot wound (about 67%), followed by the mouth (16%), forehead (7%), left temple (6%), submental (2%), and parietal region (1%). The left temple, right temple, and forehead were the sites of the gunshot entrance wounds, which were the best predictors of the handedness of the deceased (Spearman ρ = 0.149, P = 0.006). Our study showed that the direction of the bullet intracranial path in cases of suicide was even a more potent predictor of the handedness of the deceased (Spearman ρ = 0.263, P = 0.000; Wald = 149.503, P = 0.000).     

Fatal subarachnoid hemorrhage during sexual anal self-stimulation in a female: a case report

Sudden death after sexual activity is a well-known entity, but it is rare in females. Herein we present a case of fatal, massive subarachnoid hemorrhage from a ruptured berry-shaped aneurysm, during sexual anal self-stimulation. A 39-year-old woman was found dead on a couch, with the wooden handle of a spring twirl whisk inserted inside her anus and rectum as a dildo-like object. External examination was unremarkable with no signs of injuries. Intracranial examination showed a massive subarachnoid hemorrhage, up to 6 mm in thickness, especially around the brainstem and the inferior side of the brain. After removing the blood clots, the saccular aneurysm was found at the site of the bifurcation of the left internal carotid. In this case study, we underline the utility of forensic autopsy, as well as death scene investigation, in reconstructing the mechanism of death, as well as the dynamics of the event.     

Acronyms of dying versus patient autonomy

In medical practice in Germany and several other countries abbreviated orders linked to end-of-life decisions, such as DNR (do not resuscitate), are increasingly used. In order to investigate their legal status, this article gives an overview of the recently passed German law, which regulates the process of end-of-life decision-making and the use of living wills, giving primacy to patient autonomy. Concerning the risk of misinterpretation of acronyms, the article describes the impacts of such orders on patient autonomy and safety and suggests a clear systematic classification of the different DNR orders in order to investigate their legal status under the German law. Their general binding force is to be acknowledged, depending on its origination and the fulfilment of certain requirements.     

Reviews

Andrei Kuznetsov, Foreign Investment in Contemporary Russia: Managing Capital Entry. New York: St Martin's Press Inc, 1994. xiv + 188 pp., £45.00

Leslie Dienes, Istvan Dobozi & Marian Radetzki, Energy and Economic Reform in the Former Soviet Union: Implications for Production, Consumption and Exports. London: Macmillan, 1994, xiv + 246 pp., £45.00

Hilary Pilkington, Russia's Youth and its Culture. A Nation's Constructors and Constructed. London: Routledge, 1994, xiv + 358 pp., £40.00 h/b., £12.99 p/b.

Peter Gatrell, Government, Industry and Rearmament in Russia, 1900–1914: the Last Argument of Tsarism. Cambridge: Cambridge University Press, 1994, xx + 399 pp., £55.00 h/b., £19.95 p/b.

Margot Light (ed.), Troubled Friendships: Moscow's Third World Ventures. London: British Academic Press, 1993, vii + 225 pp., £39.50.

Dale F. Eickelman (ed.), Russia's Muslim Frontiers: New Directions in Cross‐cultural Analysis. Bloomington and Indianapolis, IN: Indiana University Press, 1993, ix + 225 pp.

Ljubo Sirc, Criticism of Self‐Management Still Relevant? London: Centre for Research into Communist Economies, 1994, 134 pp., £7.50.

Du?an Pokorný, Efficiency and Justice in the Industrial World: Volume 1. The Failure of the Soviet Experiment. New York, London: M. E. Sharpe, 1993, 297 pp., £53.00.

Linda J. Cook, The Soviet Social Contract and Why it Failed. Welfare Policy and Workers’ Politics from Brezhnev to Yeltsin. Cambridge, MA and London: Harvard University Press, 1994, xiii + 272 pp., £35.95/$53.95.

Henryk Kierzkowski, Marek Okolski & Stanislaw Wellisz (eds), Stabilization and Structural Adjustment in Poland. London: Routledge, 1993, xi + 314 pp., £45.00.

Stephen Whitefield (ed.) The New Institutional Architecture of Eastern Europe. Basingstoke: Macmillan, 1993, ix + 204 pp., £40.00