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961.
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Evaluation of Degradation in DNA from Males with a Quantitative Gender Typing,Endpoint PCR Multiplex
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Byron C. Smith M.S.F.S. Emily Vandegrift M.S.F.S. Valerie Mattimore Fuller Ph.D. Robert W. Allen Ph.D. School of Forensic Sciences 《Journal of forensic sciences》2015,60(2):399-408
Evidentiary samples submitted to a forensic DNA laboratory occasionally yield DNA that is degraded. Samples of intact chromosomal DNA (both nuclear and mitochondrial) were subjected to a heating protocol to induce DNA degradation. The DNAs were then analyzed using a multiplex PCR assay that amplifies targets of low and high molecular weight on the X/Y and mitochondrial chromosomes. If degradation is random, the amplification of larger DNA targets should be more adversely affected by degradation than smaller targets. In nuclear and mitochondrial DNA from a male donor, exhibiting degradation, DNA quantity estimates based upon higher molecular weight amplicons (HMW) are significantly lower than estimates made using low molecular weight (LMW) Q‐TAT amplicons. DNA degradation estimated using this approach correlated well with actual fluorescence associated with HMW and LMW STR alleles amplified from the same genomic DNA templates. Q‐TAT is thus useful not only as a quantitation tool, but also as an indicator of template degradation. 相似文献
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Saliva plus DNA from a suspect is commonly encountered in sexual assault cases on bodily swabs. However, without background knowledge, the weight of this evidence is unknown. It may indicate the presence of saliva resulting from cunnilingus, or it may represent indirect transfer. In this study, females who refrained from cunnilingus donated 43 items of underwear and 19 vaginal swabs. The samples were subjected to Phadebas®, RSID™-Saliva and mRNA profiling and were subsequently DNA-profiled to determine the prevalence of background saliva in the female population. The results report that 15.8% of females who refrained from cunnilingus were positive for saliva and a further 10.5% also had DNA from unknown source(s). These findings of the rate of indirect transfer were evaluated with the Bayesian approach, and it was found that the evidence of saliva plus a high foreign DNA source adds moderately strong support to the allegation of cunnilingus. 相似文献
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Allen Darcy W. E. Berg Chris Davidson Sinclair Potts Jason 《European Journal of Law and Economics》2022,54(1):107-125
European Journal of Law and Economics - From an epidemiological perspective, the COVID-19 pandemic is a public health crisis. From an economic perspective, it is an externality and a social cost.... 相似文献
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This study examined the potential mediating effects of social support and coping strategies on the relationship between intimate
partner violence (IPV) and psychological outcomes. A sample of 100 Caucasian women and 61 Asian women were recruited from
domestic violence agencies. Structural equation modeling was used to test the proposed model. Analysis of the combined group
revealed that there was an indirect effect of the level of violence on psychological outcomes via the mediating variables
of perceived social support and passive coping strategies. Ethnic group comparisons, however, indicated differences between
Caucasian and Asian women. In the Caucasian group, the level of violence had an indirect effect on psychological outcomes
via the mediating variables of perceived social support and passive coping strategies. In contrast, in the Asian group, the
direct effect of the level of violence on psychological outcomes was strong and significant, and the mediating roles of these
variables were not found. Research implications for practice are delineated.
This study was funded by the Institute on Domestic Violence and Sexual Assault (IDVSA) at the University of Texas at Austin. 相似文献
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