大鼠急性心肌缺血再灌流损伤c-fos mRNA原位杂交实验研究

目的 探讨急性心肌缺血和/或再灌流早期不同时间不同区域心肌细胞内原癌基因c-fos mRNA表达变化,为心肌早期缺血死后诊断提供依据。方法 复制心肌早期缺血模型,大鼠分为正常、缺血及缺血再灌流组。采用原位杂交方法结合图像分析与统计学处理方法研究心肌细胞内c-fos mRNA表达情况。结果 缺血10min再灌流30min,部分大鼠再灌流区心肌细胞胞浆内c-fos mRNA弱阳性反应,散在分布。缺血60min再灌流30min时达高峰,120min开始减弱。正常和单纯缺血组中缺血少于60min组未见有阳性表达。HE染色无明显病理改变。结论 原位杂交法检测c-fos mRNA方法灵敏特异,有望成为一项急性心肌缺血/再灌流早期损伤的诊断指标。     

中国传统法在法的现代化进程中的几个问题的研究

本文重在探讨中国传统法在现代社会中是否存在着向现代化转折的可能 ,传统法中的一些因子是否可以成为现代法文化发达的动力并丰富现代法文化的内容。笔者认为要论述传统法的现代化问题 ,首先应该对法的现代化标准有一个基本的认识 ,要区别法的现代化、法律现代化、法制现代化的不同含义。其次 ,要尽可能客观地阐述传统法的原貌 ,分析传统法产生的文化背景。在此基础上对传统法的影响进行理论分析。笔者的结论是 :中国传统法在现代社会中并没有像有些学者所“预设”的“规律”那样消亡 ,相反传统法在现实中积极的和消极的影响随处可见。只有了解传统、理解传统 ,才能正确地解释传统、更新传统。因此在法的现代化中 ,我们应该注意的是自觉、主动 ,而不是回避或被动地改造传统法。     

论医源性不合理医疗费鉴定与赔偿

目的　探讨医源性不合理医疗费产生原因、表现形式 ,以及法医学鉴定与赔偿的可行性。方法随机抽取人身损害赔偿案件 480例 (自 1996到 2 0 0 2年 ,每年 80件 ,不包括 1999年 ) ,对其医疗费进行全面的调查研究 ,并作统计分析。结果存在医源性不合理性医疗费的案件数逐年上升 ,有关费用数额也逐年增加。结论开展对医源性不合理医疗费的审查和鉴定 ,并追究侵权行为主体的法律责任具有必要性和可行性     

Demands on scientific studies in different fields of forensic medicine and forensic sciences. Traffic medicine--impaired driver: alcohol, drugs, diseases

Since human performance is a key factor in crash causation detailed knowledge of the involvement of alcohol, drugs and diseases is of fundamental interest for road safety. To better understand the effect of these influencing factors it is important to use a combination of witness observations and a range of experimental and epidemiological studies. Epidemiological approaches to research such as road-side-surveys, case-control-studies, culpability analysis, pharmaco-epidemiological studies are usually seen as being able to provide the best answers. This article discusses the relative merits of experimental and epidemiological approaches.     

Police Interviewing and Interrogation: A Self-Report Survey of Police Practices and Beliefs

By questionnaire, 631 police investigators reported on their interrogation beliefs and practices-the first such survey ever conducted. Overall, participants estimated that they were 77% accurate at truth and lie detection, that 81% of suspects waive Miranda rights, that the mean length of interrogation is 1.6 hours, and that they elicit self-incriminating statements from 68% of suspects, 4.78% from innocents. Overall, 81% felt that interrogations should be recorded. As for self-reported usage of various interrogation tactics, the most common were to physically isolate suspects, identify contradictions in suspects' accounts, establish rapport, confront suspects with evidence of their guilt, and appeal to self-interests. Results were discussed for their consistency with prior research, policy implications, and methodological shortcomings.     

Migration of a Kirschner wire used in the fixation of a subcapital humeral fracture, causing cardiac tamponade: case report and review of literature

Metallic pins and wires are frequently used for fixation of fractures and dislocations. Migration is one of the potential complications of such fixation methods. Usually, migration of the pins causes only minor complications, but if the device migrates to a vital cavity, serious damage and even death may ensue. The shoulder girdle is one of the areas in which pins and wires are mostly used, the humeral neck fractures being one of them. We report a case in which a Kirschner wire migrated from a subcapital humeral fracture site into the aorta and pericardium, causing sudden cardiac tamponade and death.     

The fate of the chemical warfare agent during DNA extraction

Forensic laboratories do not have the infrastructure to process or store contaminated DNA samples that have been recovered from a crime scene contaminated with chemical or biological warfare agents. Previous research has shown that DNA profiles can be recovered from blood exposed to several chemical warfare agents after the agent has been removed. The fate of four toxic agents, sulfur mustard, sodium 2-fluoroacetate, sarin, and diazinon, in a lysis buffer used in Promega DNA IQ extraction protocol was studied to determine if extraction would render the samples safe. Two independent analytical methods were used per agent, selected from GC-MS, 1H NMR, 19F NMR, (31)P NMR, or LC-ES MS. The methods were validated before use. Determinations were carried out in a semi-quantitative way, by direct comparison to standards. Agent levels in the elution buffer were found to be below the detectable limits for mustard, sarin, sodium 2-fluoroacetate or low (<0.02 mg/mL) for diazinon. Therefore, once extracted these DNA samples could be safely processed in a forensic laboratory.     

Analysis of 1989-1999 homicide crimes in the catchment area of the Bonn Institute of Forensic Medicine with reference to selected aspects

195 cases of homicide, grouped according to juridical assessment, were retrospectively analysed with regard to individual characteristics of perpetrators and victims, circumstances, and mode of commitment. The involvement of underage victims and perpetrators as well as differences between the urban and rural areas studied were taken into special consideration. To summarise, the majority of the offences studied was carried out by a male perpetrator with a mean age of 35 years driven by personal motives or greed in his immediate vicinity and affected family members or close acquaintances. The fatal injuries were mainly attributable to sharp or blunt force. With regard to killings of children, a higher prevalence of female victims and male assailants, emotional motives and an even higher importance of the social vicinity were found. Particularly in victims younger 12 years of age, manual modes of commitment without application of any tools were predominantly encountered. The comparison of urban and rural areas revealed a higher homicide rate in municipal areas especially of offences with a loose relationship between victims and assailants (greed, sexual assault). In contrast, rural areas exhibited higher rates of bodily harm with fatal consequences as well as offences by highly intoxicated assailants.