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41.
Cristiane Barbosa D'Oliveira Matielo M.S. Rafael Plá Matielo Lemos Ph.D. Deise Schröder Sarzi M.S. Lilian de Oliveira Machado Ph.D. Dalvan Carlos Beise Bac. Priscila Caroline Thiago Dobbler M.S. Renata Machado Castro Bac. Mauro Sander Fett Ph.D. Luiz Fernando Würdig Roesch Ph.D. Flávio Anastácio de Oliveira Camargo Ph.D. Valdir Marcos Stefenon Ph.D. 《Journal of forensic sciences》2020,65(1):259-265
DNA is one of the fastest growing tools in forensic sciences, increasing reliability in forensic reports and judgments. The use of DNA has increased in different areas of the forensic sciences, such as investigation of plant species, where plastid DNA has been used to elucidate and generate evidence in cases of traceability of genetically modified and controlled plants. Even with several advances and the practice of using DNA in forensic investigations, there are just few studies related to the identification of genetic tools for the characterization of drug and nondrug-types of Cannabis. Herein, the whole plastomes of two drug-type Cannabis are presented and have their structures compared with other Cannabis plastomes deposited in the GenBank, focusing in the forensic use of plastome sequences. The plastomes of Cannabis sativa “Brazuka” and of the hybrid Cannabis AK Royal Automatic presented general structure that does not differs from the reported for other C. sativa cultivars. A phylogenomic analyses grouped C. sativa “Brazuka” with the nondrug C. sativa cultivars, while the hybrid Cannabis AK Royal Automatic placed isolated, basal to this group. This suggests that the analysis of plastomes is useful toward genetic identification of hybrids in relation to C. sativa. 相似文献
42.
This article argues that Donald Trump’s Asian strategy hardly marks a radical transformation from Barack Obama’s ‘pivot to Asia’. This is because the ‘pivot to Asia’ never really existed. Although Obama was successful in building legitimacy for the strategy through international norms, he failed to translate his lofty rhetoric into practice. Much of his original pivot components (building a constructive relationship with China, coordinating with Asian allies and multilateralism in the Association of South East Asian Nations and the Trans-Pacific Partnership) has either failed or did not live up to expectations. Donald Trump was adamant that the Asian pivot was a bad strategy and that he would not follow it. But although he completely discarded Obama’s legitimization of the rebalance (as Trump speaks pragmatically and does not care about international norms), in actuality he has followed many of Obama’s policies, and even improved on some of them. That being said, Trump’s ‘pivot actions’ appear to be erratic, pragmatic short-term actions rather than a meticulously planned long-term strategy similar to Obama’s rebalance (which did not materialize). Thus, while Obama failed to transform the pivot into an effective strategy, neither is Trump’s effectiveness backed by a coherent Asian strategy. 相似文献
43.
Ksinan Jiskrova Gabriela Vazsonyi Alexander T. Klánová Jana Dušek Ladislav 《Journal of youth and adolescence》2019,48(3):620-634
Journal of Youth and Adolescence - Chronotype, or morningness/eveningness, has been associated with adjustment in both children and adolescents. Specifically, eveningness has been linked to... 相似文献
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45.
In recent years, Spanish local governments have come under increasing pressure to accommodate severe economic restrictions while maintaining their provision of local public services. We analyse overall cost efficiency in Spanish local governments during the period of the economic crisis (2008–2013), under four different non-parametric methodologies. Moreover, given how problematic it is to precisely define what municipalities do, we compare three different output models with various measures of quantity as well as quality. Results suggest that Spanish local government efficiency improved over the period 2008–2013 since budget expenditures (inputs) fell while local public services and facilities (outputs) were maintained. We also find evidence of the possible implications of service quality when measuring municipalities’ efficiency, and of structural differences in the average efficiency between municipalities located in different Spanish regions. Finally, our results confirm that the level and variation of efficiency scores are affected by the approach taken. 相似文献
46.
In Europe, it is relatively uncommon to find studies that investigate the criminal profiles of violent offenders, especially infrequent are those that pay special attention to offenders’ mental health/substance use disorders and histories of violence during childhood. The current research explores whether inmates who are incarcerated for violent offenses (n = 536) in Spain are different in terms of the aforementioned factors as compared to inmates incarcerated for non-violent offenses (n = 536). Multivariate logistic regression analysis revealed that violent offenders, as compared to their non-violent counterparts, were more likely to be younger, younger at the time of their first arrest, single, use alcohol, receive treatment in prison for anxiety or schizophrenia, and report physical abuse during childhood. 相似文献
47.
In 1968 he was a prominent spokesman for noncommunist intellectuals, and he later became a founding member and leader of the
human and civil rights movement “Charter 77.” The danger of totalitarianism has been his constant theme. 相似文献
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49.
Torres MC Osuna E Pérez-Cárceles MD Gómez-Zapata M Luna A 《The American journal of forensic medicine and pathology》2002,23(2):155-158
The authors evaluated the usefulness of the postmortem biochemical analysis of ionic ratios in different parts of the heart and their relation to cardiac damage caused by chest trauma, as observed by anatomopathologic study. Fifty-nine 59 cases were studied, selected from routine necropsies, and samples were taken from different sites of cardiac tissue. The cause of death was trauma in 40 cases and nontraumatic causes in 19 cases. The object of this study was to analyze the levels of Na+, K+, Ca+2, Mg+2, and Zn+2 in different zones of the heart, and the relationship between intracellular and extracellular ion ratios and the different causes of death and any anatomopathologic alterations observed. The biochemical tests revealed a possible relation between the ionic values and cause of death. Alterations in cell membrane permeability and corresponding modification of the ionic ratios were produced earlier than histologic alterations, which need longer to establish themselves whether or not they follow a traumatic process. 相似文献
50.
Baptista MJ Monsanto PV Pinho Marques EG Bermejo A Avila S Castanheira AM Margalho C Barroso M Vieira DN 《Forensic science international》2002,128(1-2):66-78
A sensitive analytical method was developed for quantitative analysis of delta(9)-tetrahydrocannabinol (delta(9)-THC), 11-nor-delta(9)-tetrahydrocannabinol-carboxylic acid (delta(9)-THC-COOH), cannabinol (CBN) and cannabidiol (CBD) in human hair. The identification of delta(9)-THC-COOH in hair would document Cannabis use more effectively than the detection of parent drug (delta(9)-THC) which might have come from environmental exposure. Ketamine was added to hair samples as internal standard for CBN and CBD. Ketoprofen was added to hair samples as internal standard for the other compounds. Samples were hydrolyzed with beta-glucuronidase/arylsulfatase for 2h at 40 degrees C. After cooling, samples were extracted with a liquid-liquid extraction procedure (with chloroform/isopropyl alcohol, after alkalinization, and n-hexane/ethyl acetate, after acidification), which was developed in our laboratory. The extracts were analysed before and after derivatization with pentafluoropropionic anhydride (PFPA) and pentafluoropropanol (PFPOH) using a Hewlett Packard gas chromatographer/mass spectrometer detector, in electron impact mode (GC/MS-EI). Derivatized delta(9)-THC-COOH was also analysed using a Hewlett Packard gas chromatographer/mass spectrometer detector, in negative ion chemical ionization mode (GC/MS-NCI) using methane as the reagent gas. Responses were linear ranging from 0.10 to 5.00 ng/mg hair for delta(9)-THC and CBN, 0.10-10.00 ng/mg hair for CBD, 0.01-5.00 ng/mg for delta(9)-THC-COOH (r(2)>0.99). The intra-assay precisions ranged from <0.01 to 12.40%. Extraction recoveries ranged from 80.9 to 104.0% for delta(9)-THC, 85.9-100.0% for delta(9)-THC-COOH, 76.7-95.8% for CBN and 71.0-94.0% for CBD. The analytical method was applied to 87 human hair samples, obtained from individuals who testified in court of having committed drug related crimes. Quantification of delta(9)-THC-COOH using GC/MS-NCI was found to be more convenient than GC/MS-EI. The latter may give rise to false negatives due to the detection limit. 相似文献