In the Aftermath of School Victimization: Links Between Authoritative School Climate and Adolescents’ Perceptions of the Negative Effects of Bullying Victimization

Journal of Youth and Adolescence - Although authoritative school climate—strict, yet fair enforcement of rules alongside strong adult support—is associated with lower rates of bullying...     

The Journey to Comprehensibility: Court Forms as the First Barrier to Accessing Justice

International Journal for the Semiotics of Law - Revue internationale de Sémiotique juridique - The article explores the comprehensibility of court forms by providing a quantitative overview...     

政府推动民营企业融资的路径——以吉林省为例

吉林省与发达省份的差距主要在于民营经济发展滞后。目前,制约吉林省民营经济发展最重要的因素是融资难。本文针对吉林省民营企业融资问题,分析了融资的现状、存在困境的成因及政府助推融资的路径,以期缓解吉林省民营企业面临的融资难、融资贵的困境。     

中草药致死非法行医案件的勘验分析

根据非法行医罪自身的特点以及实践勘验多起非法行医案件遇到问题进行剖析,拟确定技术有效的勘验和准确的鉴定思路。     

鹿结核病流行株融合基因Rv3872-Rv3874-Rv3875的原核表达

以本实验室保存的鹿体内分离并鉴定的结核病流行株为模板,利用重叠延伸剪接技术设计融合基因的引物,扩增融合基因Rv3872-Rv3874-Rv3875,构建重组表达质粒pGEX-4T-1-Rv3872-Rv3874-Rv3875;重组表达质粒被转化入大肠杆菌BL21(DE3)后,IPTG诱导表达,并采用谷胱甘肽琼脂糖亲和层析方法对目的蛋白进行纯化。基因测序结果显示,融合基因Rv3872-Rv3874-Rv3875的序列与GenBank中序列的符合率为99.89%。SDS-PAGE分析显示,融合基因在大肠杆菌中成功被诱导表达,获得以可溶形式表达的融合蛋白,该蛋白的分子质量约为60ku。Western-blot鉴定结果显示,纯化好的融合蛋白能与鹿结核病的阳性血清发生反应,说明该融合蛋白具有良好的反应原性,这一结果为它在鹿结核病血清学诊断中的应用奠定了试验基础。     

Modern Scientific Evidence Pertaining to Criminal Investigations in the Chosun Dynasty Era (1392–1897 A.C.E.) in Korea

A guidebook detailing the process of forensic investigation was written in 1440 A.C.E. It outlines the fundamentals and details of each element of criminal investigation during the era of the Chosun dynasty in Korea. Because this old guidebook was written in terms of personal experience rather than on scientific basis, it includes many fallacies from the perspective of modern forensic science. However, the book describes methods to form a scientific basis for the experiments performed. We demonstrate the modern scientific basis for ancient methods to monitor trace amounts of blood and detect lethal arsenic poisoning from a postmortem examination as described in this old forensic guidebook. Traces of blood and arsenic poisoning were detected according to the respective color changes of brownish red, due to the reaction of ferric ions in blood with acetic ions of vinegar, and dark blue, due to the reaction of silver with arsenic sulfide.     

Exploiting expressed sequence tag databases for the development and characterization of gene-derived simple sequence repeat markers in the opium poppy (Papaver somniferum L.) for forensic applications

Simple sequence repeat (SSR) markers in the opium poppy (Papaver somniferum L.) were identified from an expressed sequence tag (EST) database comprised of 20,340 sequences. In total, 2780 SSR-containing sequences were identified. The most frequent microsatellite had an AT/TA motif (37%). Twenty-two opium poppy EST-SSR markers were presently developed and polymorphisms of six markers (psom 2, 4, 12, 13, 17, and 22) were utilized in 135 individuals under narcotic control investigation. An average of three alleles per locus (range: 2-5 alleles) with a mean heterozygosity of 0.167 was detected. Six loci identified 29 unique profiles in 135 individuals. The EST-SSR markers exhibited small degrees of genetic differentiation (fixation index = 0.727, p < 0.001). Other variable markers will be needed to facilitate the forensic identification of the opium poppy for future cases. To determine the potential for cross-species amplification, six markers were tested in five Papaver genera species and two Eschscholzia genera. The psom 4 and psom 17 primer pair was transferable. This is the first study to report SSR markers of the opium poppy.     

刑事诉讼法修改如何调整证据制度

证据概念以材料说代替事实说欠妥,可以考虑三种处理方式,倾向于删去关于证据定义的规定。证据分类应注意笔录证据规定的周延性,不应仅限于列举的几种,还应包含其他侦查行为笔录及审判笔录。证据排除规则,应当根据实践需要进一步明确排除范围,尤其对"等"字应作适当界定,防止过于宽泛无法把握。直接言辞证据规则,不仅需要规定证人出庭义务及保障措施,更应当限制书面证词的使用。就行政机关所取证据的司法运用,应当明确限制行政执法机关所取言词证据用于司法。有必要规定证据裁判原则;必须遵守质证原则,反对脱离庭审秘密核实证据。     

Monitoring of clobetasol propionate and betamethasone dipropionate as undeclared steroids in cosmetic products manufactured in Korea

Some cosmetic products manufactured in Korea have been suspected to contain anti-inflammatory corticosteroids, such as clobetasol propionate and betamethasone dipropionate, for the treatment of eczema, seborrhea and psoriasis, without any indication on the label of the cosmetic products. Due to their severe side effects, such as permanent skin atopy, these two corticosteroids in cosmetic products need to be monitored from a forensic point of view. Cosmetic product samples (number of samples=47) of manufacturers charged by consumers have been collected in local and online markets of Korea, and they were validated and analyzed by a simple high performance liquid chromatography (HPLC) method with ultraviolet diode array (UV-DAD). LC-MS/MS and LC-MS were used to confirm these steroids in cosmetic samples with diagnostic ions (m/z) and isotope ratio. Linearity was studied with 0.5-10μg/mL range in both steroids. Good correlation coefficients (r(2)≥0.999) were found, and their limits of quantifications were 0.59μg/mL and 0.66μg/mL in clobetasol propionate and betamethasone dipropionate, respectively. At three different concentrations spanning the linear dynamic ranges, mean recoveries were always higher than 93%, and precisions for intra-day and inter-day analyses were both less than 3.5%. The results show 32-96.4μg/g levels of clobetasol propionate in five different cosmetic products. Also, betamethasone dipropionate in a sample was monitored at the level of 195.1μg/g. This fact reveals that some manufacturers have added these steroids in their cosmetic products to advertise the treatment effect for skin atopy. Thus, these cosmetic products need to be monitored carefully, and ultimately removed from the market.     

犬组织中艾司唑仑在甲醛溶液中的稳定性

目的 研究甲醛溶液保存的生物样品中艾司唑仑的稳定性.方法 犬以艾司唑仑37.6mg/kg剂量灌胃,2h处死,解剖取其心、肝、肾、脑并分割为1g/份,分别放置于4%甲醛溶液中固定,用高效液相色谱法测定各组织及甲醛液中的艾司唑仑含量.结果 犬心、肝、肾、脑组织随着固定在甲醛溶液中时间的延长,组织及从组织溶解到甲醛溶液中的艾...